Multidrug-loaded liposomes prevent ischemic stroke through intranasal administration
Baicalin (BA), a multi-target neuroprotective agent, has poor solubility resulting in low bioavailability. In this study, multidrug-loaded liposomes were prepared by encapsulating BA, borneol (BO) and cholic acid (CA) to prevent ischemic stroke. BBC-LP were administered intranasally (i.n.) to delive...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-06-01
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| Series: | Biomedicine & Pharmacotherapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S075333222300330X |
| _version_ | 1797837163098800128 |
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| author | Shuang Yu Dan Li Ai Shi Yu Long Jie Deng Yin Ma Xiaoqiu Li Jing Wen Yue Hu Xiaofang He Yuanyuan Wu Nan Li Mengnan Zhao |
| author_facet | Shuang Yu Dan Li Ai Shi Yu Long Jie Deng Yin Ma Xiaoqiu Li Jing Wen Yue Hu Xiaofang He Yuanyuan Wu Nan Li Mengnan Zhao |
| author_sort | Shuang Yu |
| collection | DOAJ |
| description | Baicalin (BA), a multi-target neuroprotective agent, has poor solubility resulting in low bioavailability. In this study, multidrug-loaded liposomes were prepared by encapsulating BA, borneol (BO) and cholic acid (CA) to prevent ischemic stroke. BBC-LP were administered intranasally (i.n.) to deliver into the brain for neuroprotection. Finally, potential mechanism of BBC treating ischemic stroke (IS) was explored by network pharmacology. In this study, BBC-LP was prepared by reverse evaporation method, and the encapsulation efficiency (EE) of the optimized liposomes was 42.69% and the drug loading (DL) was 6.17%. The liposomes had low mean particle size (156.62 ± 2.96 nm), polydispersity index (PDI) (0.195) and zeta potential (−0.99 mv). Compared to BBC, pharmacodynamic studies revealed that BBC-LP significantly improved neurological deficits, brain infarct volume, and cerebral pathology in MCAO rats. Toxicity studies showed that BBC-LP was not irritating to the nasal mucosa. These results suggest that BBC-LP can safely and effectively ameliorate IS injury by i.n. administration. Moreover, it's neuroprotective function may be related to the anti-apoptotic and anti-inflammatory effects exerted by phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathway and mitogen-activated protein kinase (MAPK) signaling pathway. |
| first_indexed | 2024-04-09T15:21:12Z |
| format | Article |
| id | doaj.art-646b8db051c846bca8b0f31509680731 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-04-09T15:21:12Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-646b8db051c846bca8b0f315096807312023-04-29T14:45:49ZengElsevierBiomedicine & Pharmacotherapy0753-33222023-06-01162114542Multidrug-loaded liposomes prevent ischemic stroke through intranasal administrationShuang Yu0Dan Li1Ai Shi2Yu Long3Jie Deng4Yin Ma5Xiaoqiu Li6Jing Wen7Yue Hu8Xiaofang He9Yuanyuan Wu10Nan Li11Mengnan Zhao12State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, People’s Republic of ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, People’s Republic of ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, People’s Republic of ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, People’s Republic of ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, People’s Republic of ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, People’s Republic of ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, People’s Republic of ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, People’s Republic of ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, People’s Republic of ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, People’s Republic of ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, People’s Republic of ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, People’s Republic of China; Correspondence to: School of Pharmacy, Chengdu University of Traditional Chinese Medicine, No. 1166, Liutai Avenue, Chengdu 611137, P.R. China.State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, People’s Republic of China; Correspondence to: School of Pharmacy, Chengdu University of Traditional Chinese Medicine, No. 1166, Liutai Avenue, Chengdu 611137, P.R. China.Baicalin (BA), a multi-target neuroprotective agent, has poor solubility resulting in low bioavailability. In this study, multidrug-loaded liposomes were prepared by encapsulating BA, borneol (BO) and cholic acid (CA) to prevent ischemic stroke. BBC-LP were administered intranasally (i.n.) to deliver into the brain for neuroprotection. Finally, potential mechanism of BBC treating ischemic stroke (IS) was explored by network pharmacology. In this study, BBC-LP was prepared by reverse evaporation method, and the encapsulation efficiency (EE) of the optimized liposomes was 42.69% and the drug loading (DL) was 6.17%. The liposomes had low mean particle size (156.62 ± 2.96 nm), polydispersity index (PDI) (0.195) and zeta potential (−0.99 mv). Compared to BBC, pharmacodynamic studies revealed that BBC-LP significantly improved neurological deficits, brain infarct volume, and cerebral pathology in MCAO rats. Toxicity studies showed that BBC-LP was not irritating to the nasal mucosa. These results suggest that BBC-LP can safely and effectively ameliorate IS injury by i.n. administration. Moreover, it's neuroprotective function may be related to the anti-apoptotic and anti-inflammatory effects exerted by phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathway and mitogen-activated protein kinase (MAPK) signaling pathway.http://www.sciencedirect.com/science/article/pii/S075333222300330XBaicalinLiposomeIschemic strokeIntranasal administrationNetwork pharmacology |
| spellingShingle | Shuang Yu Dan Li Ai Shi Yu Long Jie Deng Yin Ma Xiaoqiu Li Jing Wen Yue Hu Xiaofang He Yuanyuan Wu Nan Li Mengnan Zhao Multidrug-loaded liposomes prevent ischemic stroke through intranasal administration Biomedicine & Pharmacotherapy Baicalin Liposome Ischemic stroke Intranasal administration Network pharmacology |
| title | Multidrug-loaded liposomes prevent ischemic stroke through intranasal administration |
| title_full | Multidrug-loaded liposomes prevent ischemic stroke through intranasal administration |
| title_fullStr | Multidrug-loaded liposomes prevent ischemic stroke through intranasal administration |
| title_full_unstemmed | Multidrug-loaded liposomes prevent ischemic stroke through intranasal administration |
| title_short | Multidrug-loaded liposomes prevent ischemic stroke through intranasal administration |
| title_sort | multidrug loaded liposomes prevent ischemic stroke through intranasal administration |
| topic | Baicalin Liposome Ischemic stroke Intranasal administration Network pharmacology |
| url | http://www.sciencedirect.com/science/article/pii/S075333222300330X |
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