E-cigarette and food flavoring diacetyl alters airway cell morphology, inflammatory and antiviral response, and susceptibility to SARS-CoV-2
Summary Diacetyl (DA) is an α-diketone that is used to flavor microwave popcorn, coffee, and e-cigarettes. Occupational exposure to high levels of DA causes impaired lung function and obstructive airway disease. Additionally, lower levels of DA exposure dampen host defenses in vitro. Understanding D...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2022-02-01
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| Series: | Cell Death Discovery |
| Online Access: | https://doi.org/10.1038/s41420-022-00855-3 |
| _version_ | 1818280234988863488 |
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| author | Stephanie N. Langel Francine L. Kelly David M. Brass Andrew E. Nagler Dylan Carmack Joshua J. Tu Tatianna Travieso Ria Goswami Sallie R. Permar Maria Blasi Scott M. Palmer |
| author_facet | Stephanie N. Langel Francine L. Kelly David M. Brass Andrew E. Nagler Dylan Carmack Joshua J. Tu Tatianna Travieso Ria Goswami Sallie R. Permar Maria Blasi Scott M. Palmer |
| author_sort | Stephanie N. Langel |
| collection | DOAJ |
| description | Summary Diacetyl (DA) is an α-diketone that is used to flavor microwave popcorn, coffee, and e-cigarettes. Occupational exposure to high levels of DA causes impaired lung function and obstructive airway disease. Additionally, lower levels of DA exposure dampen host defenses in vitro. Understanding DA’s impact on lung epithelium is important for delineating exposure risk on lung health. In this study, we assessed the impact of DA on normal human bronchial epithelial cell (NHBEC) morphology, transcriptional profiles, and susceptibility to SARS-CoV-2 infection. Transcriptomic analysis demonstrated cilia dysregulation, an increase in hypoxia and sterile inflammation associated pathways, and decreased expression of interferon-stimulated genes after DA exposure. Additionally, DA exposure resulted in cilia loss and increased hyaluronan production. After SARS-CoV-2 infection, both genomic and subgenomic SARS-CoV-2 RNA were increased in DA vapor- compared to vehicle-exposed NHBECs. This work suggests that transcriptomic and physiologic changes induced by DA vapor exposure damage cilia and increase host susceptibility to SARS-CoV-2. |
| first_indexed | 2024-12-12T23:46:00Z |
| format | Article |
| id | doaj.art-646ba4f0dd024f46b2eed9d2d8ac948a |
| institution | Directory Open Access Journal |
| issn | 2058-7716 |
| language | English |
| last_indexed | 2024-12-12T23:46:00Z |
| publishDate | 2022-02-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Cell Death Discovery |
| spelling | doaj.art-646ba4f0dd024f46b2eed9d2d8ac948a2022-12-22T00:06:51ZengNature Publishing GroupCell Death Discovery2058-77162022-02-018111110.1038/s41420-022-00855-3E-cigarette and food flavoring diacetyl alters airway cell morphology, inflammatory and antiviral response, and susceptibility to SARS-CoV-2Stephanie N. Langel0Francine L. Kelly1David M. Brass2Andrew E. Nagler3Dylan Carmack4Joshua J. Tu5Tatianna Travieso6Ria Goswami7Sallie R. Permar8Maria Blasi9Scott M. Palmer10Duke Center for Human Systems Immunology and Department of SurgeryDuke Clinical Research Institute and Department of Medicine, Duke University Medical CenterDuke Clinical Research Institute and Department of Medicine, Duke University Medical CenterDuke Clinical Research Institute and Department of Medicine, Duke University Medical CenterDuke Clinical Research Institute and Department of Medicine, Duke University Medical CenterDuke Human Vaccine Institute, Duke University Medical CenterDuke Human Vaccine Institute, Duke University Medical CenterDepartment of Pediatrics, Weill Cornell MedicineDepartment of Pediatrics, Weill Cornell MedicineDuke Human Vaccine Institute, Duke University Medical CenterDuke Clinical Research Institute and Department of Medicine, Duke University Medical CenterSummary Diacetyl (DA) is an α-diketone that is used to flavor microwave popcorn, coffee, and e-cigarettes. Occupational exposure to high levels of DA causes impaired lung function and obstructive airway disease. Additionally, lower levels of DA exposure dampen host defenses in vitro. Understanding DA’s impact on lung epithelium is important for delineating exposure risk on lung health. In this study, we assessed the impact of DA on normal human bronchial epithelial cell (NHBEC) morphology, transcriptional profiles, and susceptibility to SARS-CoV-2 infection. Transcriptomic analysis demonstrated cilia dysregulation, an increase in hypoxia and sterile inflammation associated pathways, and decreased expression of interferon-stimulated genes after DA exposure. Additionally, DA exposure resulted in cilia loss and increased hyaluronan production. After SARS-CoV-2 infection, both genomic and subgenomic SARS-CoV-2 RNA were increased in DA vapor- compared to vehicle-exposed NHBECs. This work suggests that transcriptomic and physiologic changes induced by DA vapor exposure damage cilia and increase host susceptibility to SARS-CoV-2.https://doi.org/10.1038/s41420-022-00855-3 |
| spellingShingle | Stephanie N. Langel Francine L. Kelly David M. Brass Andrew E. Nagler Dylan Carmack Joshua J. Tu Tatianna Travieso Ria Goswami Sallie R. Permar Maria Blasi Scott M. Palmer E-cigarette and food flavoring diacetyl alters airway cell morphology, inflammatory and antiviral response, and susceptibility to SARS-CoV-2 Cell Death Discovery |
| title | E-cigarette and food flavoring diacetyl alters airway cell morphology, inflammatory and antiviral response, and susceptibility to SARS-CoV-2 |
| title_full | E-cigarette and food flavoring diacetyl alters airway cell morphology, inflammatory and antiviral response, and susceptibility to SARS-CoV-2 |
| title_fullStr | E-cigarette and food flavoring diacetyl alters airway cell morphology, inflammatory and antiviral response, and susceptibility to SARS-CoV-2 |
| title_full_unstemmed | E-cigarette and food flavoring diacetyl alters airway cell morphology, inflammatory and antiviral response, and susceptibility to SARS-CoV-2 |
| title_short | E-cigarette and food flavoring diacetyl alters airway cell morphology, inflammatory and antiviral response, and susceptibility to SARS-CoV-2 |
| title_sort | e cigarette and food flavoring diacetyl alters airway cell morphology inflammatory and antiviral response and susceptibility to sars cov 2 |
| url | https://doi.org/10.1038/s41420-022-00855-3 |
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