Cost-Effective Method to Synthesize a Fluorescent Internal DNA Standard for Automated Fragment Sizing
We describe a simple and cost-effective method for the synthesis of an internal fluorescently labeled DNA standard for fragment sizing using an automated DNA sequencer. A set of primer pairs labeled with ROX was developed to amplify 12 DNA fragments, 58–417 bp, derived from a conserved region of pla...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2001-10-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/01314st06 |