Multiplex CRISPR/Cas9-mediated knockout of the phytoene desaturase gene in Coffea canephora

Abstract Coffea canephora (2n = 2x = 22 chromosomes) is a species with extensive genetic diversity and desirable agronomic traits for coffee breeding programs. However, obtaining a new coffee cultivar through conventional breeding techniques may require more than 30 years of crossing cycles and sele...

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Main Authors: Tatiane Casarin, Natália Chagas Freitas, Renan Terassi Pinto, Jean‑Christophe Breitler, Leonardo Augusto Zebral Rodrigues, Pierre Marraccini, Hervé Etienne, Leandro Eugenio Cardamone Diniz, Alan Carvalho Andrade, Luciano Vilela Paiva
Format: Article
Language:English
Published: Nature Portfolio 2022-10-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-21566-w