The Effect of Elevated Alanine Transaminase on Non-invasive Prenatal Screening Failures
ObjectiveTo determine the effects of alanine transaminase (ALT) levels on the screening failure rates or “no calls” due to low fetal fraction (FF) to obtain a result in non-invasive prenatal screening (NIPS).MethodsNIPS by sequencing and liver enzyme measurements were performed in 7,910 pregnancies...
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2022.875588/full |
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| author | Ping Chen Longwei Qiao Sheng Zhang Jieyu Jin Jun Cao Yuqiong Zhang Haoyu Tang Zheng Yu Jingye Shi JingPing Yin Yuting Liang Xiao Wu |
| author_facet | Ping Chen Longwei Qiao Sheng Zhang Jieyu Jin Jun Cao Yuqiong Zhang Haoyu Tang Zheng Yu Jingye Shi JingPing Yin Yuting Liang Xiao Wu |
| author_sort | Ping Chen |
| collection | DOAJ |
| description | ObjectiveTo determine the effects of alanine transaminase (ALT) levels on the screening failure rates or “no calls” due to low fetal fraction (FF) to obtain a result in non-invasive prenatal screening (NIPS).MethodsNIPS by sequencing and liver enzyme measurements were performed in 7,910 pregnancies at 12–26 weeks of gestation. Univariate and multivariable regression models were used to evaluate the significant predictors of screening failure rates among maternal characteristics and relevant laboratory parameters.ResultsOf the 7,910 pregnancies that met the inclusion criteria, 134 (1.69%) had “no calls.” Multiple logistic regression analysis demonstrated that increased body mass index, ALT, prealbumin, albumin levels, and in vitro fertilization (IVF) conception rates were independently associated with screening failures. The test failure rate was higher (4.34 vs. 1.41%; P < 0.001) in IVF pregnancies relative to those with spontaneous conceptions. Meanwhile, the screening failure rates increased with increasing ALT levels from 1.05% at ≤10 U/L to 3.73% at >40 U/L. In particular, IVF pregnancies with an ALT level of >40 U/L had a higher test failure rate (9.52%). Compared with that for an ALT level of ≤10 U/L, the adjusted odds ratio of “no calls” for ALT levels of 10–20, 21–40, and >40 U/L was 1.204 [95% confidence interval (CI), 0.709–2.045], 1.529 (95% CI, 0.865–2.702), and 2.764 (95% CI, 1.500–5.093) (Ptrend < 0.001), respectively.ConclusionsIncreased ALT and IVF conceptions were associated with a higher screening failure rates in NIPS. Therefore, a feasible strategy to adjust these factors to reduce the probability of “no calls” due to low FF would be of great clinical significance. |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-04-13T17:20:25Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Medicine |
| spelling | doaj.art-64992c25dd5a4261a2ae4443c748aa902022-12-22T02:37:59ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2022-06-01910.3389/fmed.2022.875588875588The Effect of Elevated Alanine Transaminase on Non-invasive Prenatal Screening FailuresPing Chen0Longwei Qiao1Sheng Zhang2Jieyu Jin3Jun Cao4Yuqiong Zhang5Haoyu Tang6Zheng Yu7Jingye Shi8JingPing Yin9Yuting Liang10Xiao Wu11Department of Obstetrics, School of Gusu, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Nanjing Medical University, Suzhou, ChinaCenter for Reproduction and Genetics, School of Gusu, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Nanjing Medical University, Suzhou, ChinaCenter for Clinical Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, ChinaCenter for Clinical Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, ChinaCenter for Clinical Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, ChinaDepartment of Medical Laboratory, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Nanjing Medical University, Suzhou, ChinaCenter for Reproduction and Genetics, School of Gusu, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Nanjing Medical University, Suzhou, ChinaCenter for Reproduction and Genetics, School of Gusu, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Nanjing Medical University, Suzhou, ChinaCenter for Reproduction and Genetics, School of Gusu, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Nanjing Medical University, Suzhou, ChinaCenter for Clinical Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, ChinaCenter for Clinical Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, ChinaDepartment of Obstetrics, School of Gusu, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Nanjing Medical University, Suzhou, ChinaObjectiveTo determine the effects of alanine transaminase (ALT) levels on the screening failure rates or “no calls” due to low fetal fraction (FF) to obtain a result in non-invasive prenatal screening (NIPS).MethodsNIPS by sequencing and liver enzyme measurements were performed in 7,910 pregnancies at 12–26 weeks of gestation. Univariate and multivariable regression models were used to evaluate the significant predictors of screening failure rates among maternal characteristics and relevant laboratory parameters.ResultsOf the 7,910 pregnancies that met the inclusion criteria, 134 (1.69%) had “no calls.” Multiple logistic regression analysis demonstrated that increased body mass index, ALT, prealbumin, albumin levels, and in vitro fertilization (IVF) conception rates were independently associated with screening failures. The test failure rate was higher (4.34 vs. 1.41%; P < 0.001) in IVF pregnancies relative to those with spontaneous conceptions. Meanwhile, the screening failure rates increased with increasing ALT levels from 1.05% at ≤10 U/L to 3.73% at >40 U/L. In particular, IVF pregnancies with an ALT level of >40 U/L had a higher test failure rate (9.52%). Compared with that for an ALT level of ≤10 U/L, the adjusted odds ratio of “no calls” for ALT levels of 10–20, 21–40, and >40 U/L was 1.204 [95% confidence interval (CI), 0.709–2.045], 1.529 (95% CI, 0.865–2.702), and 2.764 (95% CI, 1.500–5.093) (Ptrend < 0.001), respectively.ConclusionsIncreased ALT and IVF conceptions were associated with a higher screening failure rates in NIPS. Therefore, a feasible strategy to adjust these factors to reduce the probability of “no calls” due to low FF would be of great clinical significance.https://www.frontiersin.org/articles/10.3389/fmed.2022.875588/fullnon-invasive prenatal screeningfetal fractioncell-free DNAALTmultivariable linear regression models |
| spellingShingle | Ping Chen Longwei Qiao Sheng Zhang Jieyu Jin Jun Cao Yuqiong Zhang Haoyu Tang Zheng Yu Jingye Shi JingPing Yin Yuting Liang Xiao Wu The Effect of Elevated Alanine Transaminase on Non-invasive Prenatal Screening Failures Frontiers in Medicine non-invasive prenatal screening fetal fraction cell-free DNA ALT multivariable linear regression models |
| title | The Effect of Elevated Alanine Transaminase on Non-invasive Prenatal Screening Failures |
| title_full | The Effect of Elevated Alanine Transaminase on Non-invasive Prenatal Screening Failures |
| title_fullStr | The Effect of Elevated Alanine Transaminase on Non-invasive Prenatal Screening Failures |
| title_full_unstemmed | The Effect of Elevated Alanine Transaminase on Non-invasive Prenatal Screening Failures |
| title_short | The Effect of Elevated Alanine Transaminase on Non-invasive Prenatal Screening Failures |
| title_sort | effect of elevated alanine transaminase on non invasive prenatal screening failures |
| topic | non-invasive prenatal screening fetal fraction cell-free DNA ALT multivariable linear regression models |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2022.875588/full |
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