A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution

Summary: Efficient labeling of the vasculature is important for understanding the organization of vascular networks. Here, we propose VALID, a vessel-labeling method that enables visualization of vascular networks with tissue clearing and light-sheet microscopy. VALID transforms traditional lipophil...

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Bibliographic Details
Main Authors: Jingtan Zhu, Xiaomei Liu, Jianyi Xu, Yating Deng, Pingfu Wang, Zhang Liu, Qihang Yang, Dongyu Li, Tingting Yu, Dan Zhu
Format: Article
Language:English
Published: Elsevier 2023-02-01
Series:Cell Reports: Methods
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2667237523000188