An atlas of gross and histologic lesions and immunohistochemical immunoreactivity during the temporal progression of aerosolized Lassa virus induced hemorrhagic fever in cynomolgus macaques
Lassa virus (LASV) causes an acute multisystemic hemorrhagic fever in humans known as Lassa fever, which is endemic in several African countries. This manuscript focuses on the progression of disease in cynomolgus macaques challenged with aerosolized LASV and serially sampled for the development and...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1341891/full |
| _version_ | 1797319415919804416 |
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| author | Forrest Bohler Kathleen Cashman Eric Wilkinson Joshua C. Johnson Kyle Rosenke Josh Shamblin Lisa Hensley Anna Honko Carl Shaia |
| author_facet | Forrest Bohler Kathleen Cashman Eric Wilkinson Joshua C. Johnson Kyle Rosenke Josh Shamblin Lisa Hensley Anna Honko Carl Shaia |
| author_sort | Forrest Bohler |
| collection | DOAJ |
| description | Lassa virus (LASV) causes an acute multisystemic hemorrhagic fever in humans known as Lassa fever, which is endemic in several African countries. This manuscript focuses on the progression of disease in cynomolgus macaques challenged with aerosolized LASV and serially sampled for the development and progression of gross and histopathologic lesions. Gross lesions were first noted in tissues on day 6 and persisted throughout day 12. Viremia and histologic lesions were first noted on day 6 commencing with the pulmonary system and hemolymphatic system and progressing at later time points to include all systems. Immunoreactivity to LASV antigen was first observed in the lungs of one macaque on day 3 and appeared localized to macrophages with an increase at later time points to include immunoreactivity in all organ systems. Additionally, this manuscript will serve as a detailed atlas of histopathologic lesions and disease progression for comparison to other animal models of aerosolized Arenaviral disease. |
| first_indexed | 2024-03-08T04:06:34Z |
| format | Article |
| id | doaj.art-64ea00c679564d18bd782986357aff99 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-03-08T04:06:34Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-64ea00c679564d18bd782986357aff992024-02-09T05:01:01ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882024-02-011410.3389/fcimb.2024.13418911341891An atlas of gross and histologic lesions and immunohistochemical immunoreactivity during the temporal progression of aerosolized Lassa virus induced hemorrhagic fever in cynomolgus macaquesForrest Bohler0Kathleen Cashman1Eric Wilkinson2Joshua C. Johnson3Kyle Rosenke4Josh Shamblin5Lisa Hensley6Anna Honko7Carl Shaia8Laboratory of Virology, National Institute of Allergy and Infectious Diseases, Division of Intramural Research, National Institutes of Health (NIH), Hamilton, MT, United StatesVirology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Frederick, MD, United StatesVirology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Frederick, MD, United StatesVirology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Frederick, MD, United StatesLaboratory of Virology, National Institute of Allergy and Infectious Diseases, Division of Intramural Research, National Institutes of Health (NIH), Hamilton, MT, United StatesVirology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Frederick, MD, United StatesZoonotic and Emerging Disease Research Unit, United States Department of Agriculture (USDA), Manhattan, KS, United StatesVirology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Frederick, MD, United StatesRocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases, Division of Intramural Research, National Institutes of Health (NIH), Hamilton, MT, United StatesLassa virus (LASV) causes an acute multisystemic hemorrhagic fever in humans known as Lassa fever, which is endemic in several African countries. This manuscript focuses on the progression of disease in cynomolgus macaques challenged with aerosolized LASV and serially sampled for the development and progression of gross and histopathologic lesions. Gross lesions were first noted in tissues on day 6 and persisted throughout day 12. Viremia and histologic lesions were first noted on day 6 commencing with the pulmonary system and hemolymphatic system and progressing at later time points to include all systems. Immunoreactivity to LASV antigen was first observed in the lungs of one macaque on day 3 and appeared localized to macrophages with an increase at later time points to include immunoreactivity in all organ systems. Additionally, this manuscript will serve as a detailed atlas of histopathologic lesions and disease progression for comparison to other animal models of aerosolized Arenaviral disease.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1341891/fullLassa virusLassa fevercynomolgus macaquesnon-human primatehistologic lesionimmunohistochemistry |
| spellingShingle | Forrest Bohler Kathleen Cashman Eric Wilkinson Joshua C. Johnson Kyle Rosenke Josh Shamblin Lisa Hensley Anna Honko Carl Shaia An atlas of gross and histologic lesions and immunohistochemical immunoreactivity during the temporal progression of aerosolized Lassa virus induced hemorrhagic fever in cynomolgus macaques Frontiers in Cellular and Infection Microbiology Lassa virus Lassa fever cynomolgus macaques non-human primate histologic lesion immunohistochemistry |
| title | An atlas of gross and histologic lesions and immunohistochemical immunoreactivity during the temporal progression of aerosolized Lassa virus induced hemorrhagic fever in cynomolgus macaques |
| title_full | An atlas of gross and histologic lesions and immunohistochemical immunoreactivity during the temporal progression of aerosolized Lassa virus induced hemorrhagic fever in cynomolgus macaques |
| title_fullStr | An atlas of gross and histologic lesions and immunohistochemical immunoreactivity during the temporal progression of aerosolized Lassa virus induced hemorrhagic fever in cynomolgus macaques |
| title_full_unstemmed | An atlas of gross and histologic lesions and immunohistochemical immunoreactivity during the temporal progression of aerosolized Lassa virus induced hemorrhagic fever in cynomolgus macaques |
| title_short | An atlas of gross and histologic lesions and immunohistochemical immunoreactivity during the temporal progression of aerosolized Lassa virus induced hemorrhagic fever in cynomolgus macaques |
| title_sort | atlas of gross and histologic lesions and immunohistochemical immunoreactivity during the temporal progression of aerosolized lassa virus induced hemorrhagic fever in cynomolgus macaques |
| topic | Lassa virus Lassa fever cynomolgus macaques non-human primate histologic lesion immunohistochemistry |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1341891/full |
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