Daytime administration of melatonin has better protective effects on bone loss in ovariectomized rats
Abstract Objective To explore the difference in the protective effects of intraperitoneal injection of exogenous melatonin of daytime or nighttime on bone loss in ovariectomized (OVX) rats. Methods After bilateral ovariectomy and sham surgery, 40 rats were randomly divided into four groups: sham ope...
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| Format: | Article |
| Language: | English |
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BMC
2023-03-01
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| Series: | Journal of Orthopaedic Surgery and Research |
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| Online Access: | https://doi.org/10.1186/s13018-023-03695-8 |
| _version_ | 1797859886228307968 |
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| author | Tian-lin Li He-dong Liu Mao-xian Ren Zhi Zhou Wen-kai Jiang Min Yang |
| author_facet | Tian-lin Li He-dong Liu Mao-xian Ren Zhi Zhou Wen-kai Jiang Min Yang |
| author_sort | Tian-lin Li |
| collection | DOAJ |
| description | Abstract Objective To explore the difference in the protective effects of intraperitoneal injection of exogenous melatonin of daytime or nighttime on bone loss in ovariectomized (OVX) rats. Methods After bilateral ovariectomy and sham surgery, 40 rats were randomly divided into four groups: sham operation group (Sham), ovariectomy (OVX), and daytime melatonin injection group (OVX + DMLT, 9:00, 30 mg/kg/d) and nighttime injection of melatonin (OVX + NMLT, 22:00, 30 mg/kg/d). After 12 weeks of treatment, the rats were sacrificed. The distal femur, blood and femoral marrow cavity contents were saved. The rest of the samples were tested by Micro-CT, histology, biomechanics and molecular biology. Blood was used for bone metabolism marker measurements. CCK-8, ROS, and Cell apoptosis are performed using MC3E3-T1 cells. Results Compared with treatment at night, the bone mass of the OVX rats was significantly increased after the daytime administration. All microscopic parameters of trabecular bone increased, only Tb.Sp decreased. Histologically, the bone microarchitecture of the OVX + DMLT was also more dense than the bone microarchitecture of the OVX + LMLT. In the biomechanical experiment, the femur samples of the day treatment group were able to withstand greater loads and deformation. In molecular biology experiments, bone formation-related molecules increased, while bone resorption-related molecules decreased. After treatment with melatonin administration at night, the expression of MT-1β was significantly decreased. In cell experiments, the MC3E3-T1 cells treated with low-dose MLT had higher cell viability and greater efficiency in inhibiting ROS production than the MC3E3-T1 cells treated with high-dose MLT, which in turn more effectively inhibited apoptosis. Conclusion Daytime administration of melatonin acquires better protective effects on bone loss than night in OVX rats. |
| first_indexed | 2024-04-09T21:36:49Z |
| format | Article |
| id | doaj.art-65001834b567402cbf1b67932e4c9149 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-09T21:36:49Z |
| publishDate | 2023-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-65001834b567402cbf1b67932e4c91492023-03-26T11:14:50ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2023-03-0118111310.1186/s13018-023-03695-8Daytime administration of melatonin has better protective effects on bone loss in ovariectomized ratsTian-lin Li0He-dong Liu1Mao-xian Ren2Zhi Zhou3Wen-kai Jiang4Min Yang5Department of Trauma Orthopedics, The First Affiliated Hospital of Wannan Medical College, Yijishan HospitalDepartment of Trauma Orthopedics, The First Affiliated Hospital of Wannan Medical College, Yijishan HospitalDepartment of Trauma Orthopedics, The First Affiliated Hospital of Wannan Medical College, Yijishan HospitalDepartment of Trauma Orthopedics, The First Affiliated Hospital of Wannan Medical College, Yijishan HospitalDepartment of Trauma Orthopedics, The First Affiliated Hospital of Wannan Medical College, Yijishan HospitalDepartment of Trauma Orthopedics, The First Affiliated Hospital of Wannan Medical College, Yijishan HospitalAbstract Objective To explore the difference in the protective effects of intraperitoneal injection of exogenous melatonin of daytime or nighttime on bone loss in ovariectomized (OVX) rats. Methods After bilateral ovariectomy and sham surgery, 40 rats were randomly divided into four groups: sham operation group (Sham), ovariectomy (OVX), and daytime melatonin injection group (OVX + DMLT, 9:00, 30 mg/kg/d) and nighttime injection of melatonin (OVX + NMLT, 22:00, 30 mg/kg/d). After 12 weeks of treatment, the rats were sacrificed. The distal femur, blood and femoral marrow cavity contents were saved. The rest of the samples were tested by Micro-CT, histology, biomechanics and molecular biology. Blood was used for bone metabolism marker measurements. CCK-8, ROS, and Cell apoptosis are performed using MC3E3-T1 cells. Results Compared with treatment at night, the bone mass of the OVX rats was significantly increased after the daytime administration. All microscopic parameters of trabecular bone increased, only Tb.Sp decreased. Histologically, the bone microarchitecture of the OVX + DMLT was also more dense than the bone microarchitecture of the OVX + LMLT. In the biomechanical experiment, the femur samples of the day treatment group were able to withstand greater loads and deformation. In molecular biology experiments, bone formation-related molecules increased, while bone resorption-related molecules decreased. After treatment with melatonin administration at night, the expression of MT-1β was significantly decreased. In cell experiments, the MC3E3-T1 cells treated with low-dose MLT had higher cell viability and greater efficiency in inhibiting ROS production than the MC3E3-T1 cells treated with high-dose MLT, which in turn more effectively inhibited apoptosis. Conclusion Daytime administration of melatonin acquires better protective effects on bone loss than night in OVX rats.https://doi.org/10.1186/s13018-023-03695-8OsteoporosisMelatoninBone lossBone massEstrogen deficiency |
| spellingShingle | Tian-lin Li He-dong Liu Mao-xian Ren Zhi Zhou Wen-kai Jiang Min Yang Daytime administration of melatonin has better protective effects on bone loss in ovariectomized rats Journal of Orthopaedic Surgery and Research Osteoporosis Melatonin Bone loss Bone mass Estrogen deficiency |
| title | Daytime administration of melatonin has better protective effects on bone loss in ovariectomized rats |
| title_full | Daytime administration of melatonin has better protective effects on bone loss in ovariectomized rats |
| title_fullStr | Daytime administration of melatonin has better protective effects on bone loss in ovariectomized rats |
| title_full_unstemmed | Daytime administration of melatonin has better protective effects on bone loss in ovariectomized rats |
| title_short | Daytime administration of melatonin has better protective effects on bone loss in ovariectomized rats |
| title_sort | daytime administration of melatonin has better protective effects on bone loss in ovariectomized rats |
| topic | Osteoporosis Melatonin Bone loss Bone mass Estrogen deficiency |
| url | https://doi.org/10.1186/s13018-023-03695-8 |
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