Ultrasensitive and visual detection of human norovirus genotype GII.4 or GII.17 using CRISPR-Cas12a assay

Ultrasensitive and visual detection of human norovirus genotype GII.4 or GII.17 using CRISPR-Cas12a assay

Abstract Background Integrating CRISPR-Cas12a sensors with isothermal signal amplification can be exploited to develop low-cost, disposable, and ultrasensitive assays for the diagnostics of human pathogens. Methods RT-RAA-Cas12a-mediated real-time or end-point fluorescent and lateral flow strip (LFS...

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Bibliographic Details
Main Authors: Weidong Qian, Jie Huang, Ting Wang, Cheng Fan, Jie Kang, Qian Zhang, Yongdong Li, Si Chen
Format: Article
Language:English
Published: BMC 2022-09-01
Series:Virology Journal
Subjects:
CRISPR-Cas12a
RT-RAA
Diagnostics
Human norovirus
GII.4 or GII.17
Online Access:https://doi.org/10.1186/s12985-022-01878-z

Internet

https://doi.org/10.1186/s12985-022-01878-z

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