Adding Appropriate Fiber in Diet Increases Diversity and Metabolic Capacity of Distal Gut Microbiota Without Altering Fiber Digestibility and Growth Rate of Finishing Pig
The digestion ability of pigs to dietary fiber is derived from their intestinal microbiota, especially hindgut microbiota. However, tolerance of pigs to high dietary fiber and the changes of microbiota profile with fiber levels are still unclear. To investigate the changes of gut microbiota with die...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.00533/full |
| _version_ | 1818154675841531904 |
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| author | Guang Pu Guang Pu Pinghua Li Pinghua Li Pinghua Li Pinghua Li Taoran Du Taoran Du Qing Niu Lijuan Fan Lijuan Fan Huan Wang Huan Wang Hang Liu Hang Liu Kaijun Li Kaijun Li Peipei Niu Chengwu Wu Chengwu Wu Wuduo Zhou Wuduo Zhou Ruihua Huang Ruihua Huang Ruihua Huang |
| author_facet | Guang Pu Guang Pu Pinghua Li Pinghua Li Pinghua Li Pinghua Li Taoran Du Taoran Du Qing Niu Lijuan Fan Lijuan Fan Huan Wang Huan Wang Hang Liu Hang Liu Kaijun Li Kaijun Li Peipei Niu Chengwu Wu Chengwu Wu Wuduo Zhou Wuduo Zhou Ruihua Huang Ruihua Huang Ruihua Huang |
| author_sort | Guang Pu |
| collection | DOAJ |
| description | The digestion ability of pigs to dietary fiber is derived from their intestinal microbiota, especially hindgut microbiota. However, tolerance of pigs to high dietary fiber and the changes of microbiota profile with fiber levels are still unclear. To investigate the changes of gut microbiota with dietary fiber and its relationship with fiber digestibility, we conducted comparative analyses of growth rate, apparent fiber digestibility, gut microbiota and volatile fatty acid (VFA) profiles in Chinese Suhuai pigs feeding diets with different defatted rice bran (DFRB) fiber levels. We found that dietary fiber level had no effect on the growth rate of Suhuai pigs. Although the apparent digestibility of Cellulose, insoluble dietary fiber (IDF) and total dietary fiber (TDF) decreased with dietary fiber level, we found that the apparent digestibility of Cellulose, IDF and TDF of Suhuai pigs was not changed when provided with diet containing 19.10% TDF (as feed basis). The pigs provided with diet containing 19.10% TDF had higher microbial richness, proportions of several fiber-degrading bacteria taxa at genus level and predicted microbial functions (such as carbohydrate metabolism, energy metabolism) in cecum compared to those fed with basal diet. In addition, the fiber-induced increasing of fiber-degrading bacteria promoted the VFAs metabolism, which potentially helped Suhuai pigs to maintain growth rate. However, as TDF reached to 24.11% (as feed basis), the apparent digestibility of fiber decreased and the positive effect on intestine microbiota in caecum were absent. Together, our data suggest that appropriate fiber level could increase the diversity and metabolic capacity of distal gut microbiota to improve the utilization efficiency of fiber resources without altering the growth rate of pigs. |
| first_indexed | 2024-12-11T14:30:17Z |
| format | Article |
| id | doaj.art-6518f030676d4d4598edb9cfe702d2dc |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T14:30:17Z |
| publishDate | 2020-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-6518f030676d4d4598edb9cfe702d2dc2022-12-22T01:02:28ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-04-011110.3389/fmicb.2020.00533514929Adding Appropriate Fiber in Diet Increases Diversity and Metabolic Capacity of Distal Gut Microbiota Without Altering Fiber Digestibility and Growth Rate of Finishing PigGuang Pu0Guang Pu1Pinghua Li2Pinghua Li3Pinghua Li4Pinghua Li5Taoran Du6Taoran Du7Qing Niu8Lijuan Fan9Lijuan Fan10Huan Wang11Huan Wang12Hang Liu13Hang Liu14Kaijun Li15Kaijun Li16Peipei Niu17Chengwu Wu18Chengwu Wu19Wuduo Zhou20Wuduo Zhou21Ruihua Huang22Ruihua Huang23Ruihua Huang24Institute of Swine Science, Nanjing Agricultural University, Nanjing, ChinaHuaian Academy, Nanjing Agricultural University, Huaian, ChinaInstitute of Swine Science, Nanjing Agricultural University, Nanjing, ChinaHuaian Academy, Nanjing Agricultural University, Huaian, ChinaIndustrial Technology System Integration Innovation Center of Jiangsu Modern Agriculture (PIG), Nanjing, ChinaNanjing Agricultural University’s New Rural Research and Development Corporation of Huaian City, Huaian, ChinaInstitute of Swine Science, Nanjing Agricultural University, Nanjing, ChinaHuaian Academy, Nanjing Agricultural University, Huaian, ChinaInstitute of Swine Science, Nanjing Agricultural University, Nanjing, ChinaInstitute of Swine Science, Nanjing Agricultural University, Nanjing, ChinaHuaian Academy, Nanjing Agricultural University, Huaian, ChinaInstitute of Swine Science, Nanjing Agricultural University, Nanjing, ChinaHuaian Academy, Nanjing Agricultural University, Huaian, ChinaInstitute of Swine Science, Nanjing Agricultural University, Nanjing, ChinaHuaian Academy, Nanjing Agricultural University, Huaian, ChinaInstitute of Swine Science, Nanjing Agricultural University, Nanjing, ChinaHuaian Academy, Nanjing Agricultural University, Huaian, ChinaHuaian Academy, Nanjing Agricultural University, Huaian, ChinaIndustrial Technology System Integration Innovation Center of Jiangsu Modern Agriculture (PIG), Nanjing, ChinaNanjing Agricultural University’s New Rural Research and Development Corporation of Huaian City, Huaian, ChinaInstitute of Swine Science, Nanjing Agricultural University, Nanjing, ChinaIndustrial Technology System Integration Innovation Center of Jiangsu Modern Agriculture (PIG), Nanjing, ChinaInstitute of Swine Science, Nanjing Agricultural University, Nanjing, ChinaHuaian Academy, Nanjing Agricultural University, Huaian, ChinaIndustrial Technology System Integration Innovation Center of Jiangsu Modern Agriculture (PIG), Nanjing, ChinaThe digestion ability of pigs to dietary fiber is derived from their intestinal microbiota, especially hindgut microbiota. However, tolerance of pigs to high dietary fiber and the changes of microbiota profile with fiber levels are still unclear. To investigate the changes of gut microbiota with dietary fiber and its relationship with fiber digestibility, we conducted comparative analyses of growth rate, apparent fiber digestibility, gut microbiota and volatile fatty acid (VFA) profiles in Chinese Suhuai pigs feeding diets with different defatted rice bran (DFRB) fiber levels. We found that dietary fiber level had no effect on the growth rate of Suhuai pigs. Although the apparent digestibility of Cellulose, insoluble dietary fiber (IDF) and total dietary fiber (TDF) decreased with dietary fiber level, we found that the apparent digestibility of Cellulose, IDF and TDF of Suhuai pigs was not changed when provided with diet containing 19.10% TDF (as feed basis). The pigs provided with diet containing 19.10% TDF had higher microbial richness, proportions of several fiber-degrading bacteria taxa at genus level and predicted microbial functions (such as carbohydrate metabolism, energy metabolism) in cecum compared to those fed with basal diet. In addition, the fiber-induced increasing of fiber-degrading bacteria promoted the VFAs metabolism, which potentially helped Suhuai pigs to maintain growth rate. However, as TDF reached to 24.11% (as feed basis), the apparent digestibility of fiber decreased and the positive effect on intestine microbiota in caecum were absent. Together, our data suggest that appropriate fiber level could increase the diversity and metabolic capacity of distal gut microbiota to improve the utilization efficiency of fiber resources without altering the growth rate of pigs.https://www.frontiersin.org/article/10.3389/fmicb.2020.00533/fullfiber intakecaecuminsoluble dietary fiber16S rRNA gene sequencingVFA |
| spellingShingle | Guang Pu Guang Pu Pinghua Li Pinghua Li Pinghua Li Pinghua Li Taoran Du Taoran Du Qing Niu Lijuan Fan Lijuan Fan Huan Wang Huan Wang Hang Liu Hang Liu Kaijun Li Kaijun Li Peipei Niu Chengwu Wu Chengwu Wu Wuduo Zhou Wuduo Zhou Ruihua Huang Ruihua Huang Ruihua Huang Adding Appropriate Fiber in Diet Increases Diversity and Metabolic Capacity of Distal Gut Microbiota Without Altering Fiber Digestibility and Growth Rate of Finishing Pig Frontiers in Microbiology fiber intake caecum insoluble dietary fiber 16S rRNA gene sequencing VFA |
| title | Adding Appropriate Fiber in Diet Increases Diversity and Metabolic Capacity of Distal Gut Microbiota Without Altering Fiber Digestibility and Growth Rate of Finishing Pig |
| title_full | Adding Appropriate Fiber in Diet Increases Diversity and Metabolic Capacity of Distal Gut Microbiota Without Altering Fiber Digestibility and Growth Rate of Finishing Pig |
| title_fullStr | Adding Appropriate Fiber in Diet Increases Diversity and Metabolic Capacity of Distal Gut Microbiota Without Altering Fiber Digestibility and Growth Rate of Finishing Pig |
| title_full_unstemmed | Adding Appropriate Fiber in Diet Increases Diversity and Metabolic Capacity of Distal Gut Microbiota Without Altering Fiber Digestibility and Growth Rate of Finishing Pig |
| title_short | Adding Appropriate Fiber in Diet Increases Diversity and Metabolic Capacity of Distal Gut Microbiota Without Altering Fiber Digestibility and Growth Rate of Finishing Pig |
| title_sort | adding appropriate fiber in diet increases diversity and metabolic capacity of distal gut microbiota without altering fiber digestibility and growth rate of finishing pig |
| topic | fiber intake caecum insoluble dietary fiber 16S rRNA gene sequencing VFA |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.00533/full |
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