In vitro Antioxidant and Anti-Inflammatory Activities of Aqueous Leaf Extract of Alchornea cordifolia
The present study was undertaken to evaluate antioxidant and anti‐inflammatory activities of aqueous leaf extract of Alchornea cordifolia by in vitro methods. The aqueous extract is prepared by dissolving ground plant materials (100g) in 1 L of distilled for 48 hours, filtered, and then dried using...
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| Format: | Article |
| Language: | English |
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Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)
2023-02-01
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| Series: | Journal of Applied Sciences and Environmental Management |
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| Online Access: | https://www.ajol.info/index.php/jasem/article/view/242521 |
| _version_ | 1797231272027750400 |
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| author | O. Oruka F. I. Achuba |
| author_facet | O. Oruka F. I. Achuba |
| author_sort | O. Oruka |
| collection | DOAJ |
| description |
The present study was undertaken to evaluate antioxidant and anti‐inflammatory activities of aqueous leaf extract of Alchornea cordifolia by in vitro methods. The aqueous extract is prepared by dissolving ground plant materials (100g) in 1 L of distilled for 48 hours, filtered, and then dried using rotary evaporator before it was used for the pharmacological investigations. Standard phytochemical methods were used to test for the presence of phytoactive compounds in the leaf, in vitro antioxidant and anti-inflammatory activities were also assayed for using various methods. The qualitative screening of the phytochemical constituents of the aqueous extracts of Alchornea cordifolia leaves showed the presence of Phenols, tannins, flavonoids, alkaloids and cardiac glycosides while the quantitative phytochemical analysis revealed the presence of tannin, alkaloid, flavonoids and phenol. Tannins had the highest concentration, while the lowest was flavonoids. The in-vitro antioxidant study revealed that aqueous leaf extract of Alchornea cordifolia possesses antioxidant activity in a dose-dependent manner against DPPH, reducing power, total antioxidant capacity and nitric oxide activity while the outcome of the in-vitro anti-inflammatory assay revealed that the antiproteinase, membrane stabilization and albumin denaturation activities of the extracts significantly (p<0.05) increased in a concentration-dependent manner. In conclusion, the leaf of Alchornea cordifolia is rich in phytochemical substances. This could be responsible for the in-vitro antioxidants and anti-inflammatory activities.
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| first_indexed | 2024-04-24T15:41:44Z |
| format | Article |
| id | doaj.art-6551ae9b693c4ef983e15d1a121e7dc3 |
| institution | Directory Open Access Journal |
| issn | 2659-1502 2659-1499 |
| language | English |
| last_indexed | 2024-04-24T15:41:44Z |
| publishDate | 2023-02-01 |
| publisher | Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP) |
| record_format | Article |
| series | Journal of Applied Sciences and Environmental Management |
| spelling | doaj.art-6551ae9b693c4ef983e15d1a121e7dc32024-04-01T21:34:15ZengJoint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)Journal of Applied Sciences and Environmental Management2659-15022659-14992023-02-0127210.4314/jasem.v27i2.16In vitro Antioxidant and Anti-Inflammatory Activities of Aqueous Leaf Extract of Alchornea cordifoliaO. OrukaF. I. Achuba The present study was undertaken to evaluate antioxidant and anti‐inflammatory activities of aqueous leaf extract of Alchornea cordifolia by in vitro methods. The aqueous extract is prepared by dissolving ground plant materials (100g) in 1 L of distilled for 48 hours, filtered, and then dried using rotary evaporator before it was used for the pharmacological investigations. Standard phytochemical methods were used to test for the presence of phytoactive compounds in the leaf, in vitro antioxidant and anti-inflammatory activities were also assayed for using various methods. The qualitative screening of the phytochemical constituents of the aqueous extracts of Alchornea cordifolia leaves showed the presence of Phenols, tannins, flavonoids, alkaloids and cardiac glycosides while the quantitative phytochemical analysis revealed the presence of tannin, alkaloid, flavonoids and phenol. Tannins had the highest concentration, while the lowest was flavonoids. The in-vitro antioxidant study revealed that aqueous leaf extract of Alchornea cordifolia possesses antioxidant activity in a dose-dependent manner against DPPH, reducing power, total antioxidant capacity and nitric oxide activity while the outcome of the in-vitro anti-inflammatory assay revealed that the antiproteinase, membrane stabilization and albumin denaturation activities of the extracts significantly (p<0.05) increased in a concentration-dependent manner. In conclusion, the leaf of Alchornea cordifolia is rich in phytochemical substances. This could be responsible for the in-vitro antioxidants and anti-inflammatory activities. https://www.ajol.info/index.php/jasem/article/view/242521Alchornea cordifolia;antioxidants;anti-inflammatory;phytochemicals |
| spellingShingle | O. Oruka F. I. Achuba In vitro Antioxidant and Anti-Inflammatory Activities of Aqueous Leaf Extract of Alchornea cordifolia Journal of Applied Sciences and Environmental Management Alchornea cordifolia; antioxidants; anti-inflammatory; phytochemicals |
| title | In vitro Antioxidant and Anti-Inflammatory Activities of Aqueous Leaf Extract of Alchornea cordifolia |
| title_full | In vitro Antioxidant and Anti-Inflammatory Activities of Aqueous Leaf Extract of Alchornea cordifolia |
| title_fullStr | In vitro Antioxidant and Anti-Inflammatory Activities of Aqueous Leaf Extract of Alchornea cordifolia |
| title_full_unstemmed | In vitro Antioxidant and Anti-Inflammatory Activities of Aqueous Leaf Extract of Alchornea cordifolia |
| title_short | In vitro Antioxidant and Anti-Inflammatory Activities of Aqueous Leaf Extract of Alchornea cordifolia |
| title_sort | in vitro antioxidant and anti inflammatory activities of aqueous leaf extract of alchornea cordifolia |
| topic | Alchornea cordifolia; antioxidants; anti-inflammatory; phytochemicals |
| url | https://www.ajol.info/index.php/jasem/article/view/242521 |
| work_keys_str_mv | AT ooruka invitroantioxidantandantiinflammatoryactivitiesofaqueousleafextractofalchorneacordifolia AT fiachuba invitroantioxidantandantiinflammatoryactivitiesofaqueousleafextractofalchorneacordifolia |