Hydrogen peroxide enhances transforming growth factor beta-2 induced epithelial–mesenchymal transition of ARPE-19 cells
Abstract Background In the pathophysiology of the retina, the epithelial–mesenchymal transition and oxidative stress play key roles. To find a relationship between oxidative stress and diabetic retinopathy or proliferative retinopathy, we used an in-silico approach. We also used an in vitro investig...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-11-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43088-023-00421-5 |
| _version_ | 1797630022008176640 |
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| author | Brijesh Gelat Pooja Rathaur Pooja Malaviya Binita Patel Abhay R. Vasavada S. R. Kaid Johar |
| author_facet | Brijesh Gelat Pooja Rathaur Pooja Malaviya Binita Patel Abhay R. Vasavada S. R. Kaid Johar |
| author_sort | Brijesh Gelat |
| collection | DOAJ |
| description | Abstract Background In the pathophysiology of the retina, the epithelial–mesenchymal transition and oxidative stress play key roles. To find a relationship between oxidative stress and diabetic retinopathy or proliferative retinopathy, we used an in-silico approach. We also used an in vitro investigation to examine the synergistic effect of transforming growth factors-β2 (TGFβ2) and hydrogen peroxide (H2O2) on the epithelial–mesenchymal transition (EMT) of ARPE-19 cells, analyzing cell survival, cell migration by wound healing, and expression epithelial–mesenchymal transition markers at the protein and mRNA levels. Results For 24 h, human retinal pigment epithelial cells (ARPE-19) were cultured in standard conditions and subjected to various concentrations of TGFβ2, H2O2, and their combinations. According to a bioinformatics study, signaling pathways including hypoxia-inducible factor (HIF-1), forkhead box O (FoxO), phosphoinositide-3-kinase (PI3K-Akt), and transforming growth factors-β (TGFβ-signaling), both growth factors and oxidative stress-associated proteins play a significant role in the development of retinopathies. In vitro studies showed that oxidative stress induced by H2O2 and TGFβ2 enhances the epithelial–mesenchymal transition of ARPE-19 cells. Conclusions Hydrogen peroxide enhances the TGFβ2-mediated epithelial–mesenchymal transition of human retinal pigment epithelial cells, or at least in cell line ARPE-19 cells. Graphical abstract |
| first_indexed | 2024-03-11T11:02:11Z |
| format | Article |
| id | doaj.art-6552ef93e53d4b9cbaf94c5299493572 |
| institution | Directory Open Access Journal |
| issn | 2314-8543 |
| language | English |
| last_indexed | 2024-03-11T11:02:11Z |
| publishDate | 2023-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-6552ef93e53d4b9cbaf94c52994935722023-11-12T12:26:52ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85432023-11-0112111510.1186/s43088-023-00421-5Hydrogen peroxide enhances transforming growth factor beta-2 induced epithelial–mesenchymal transition of ARPE-19 cellsBrijesh Gelat0Pooja Rathaur1Pooja Malaviya2Binita Patel3Abhay R. Vasavada4S. R. Kaid Johar5Department of Zoology, Biomedical Technology, Human Genetics, and WBC, School of Sciences, Gujarat UniversityIladevi Cataract and IOL Research CenterIladevi Cataract and IOL Research CenterDepartment of Life Sciences, School of Sciences, Gujarat UniversityIladevi Cataract and IOL Research CenterDepartment of Zoology, Biomedical Technology, Human Genetics, and WBC, School of Sciences, Gujarat UniversityAbstract Background In the pathophysiology of the retina, the epithelial–mesenchymal transition and oxidative stress play key roles. To find a relationship between oxidative stress and diabetic retinopathy or proliferative retinopathy, we used an in-silico approach. We also used an in vitro investigation to examine the synergistic effect of transforming growth factors-β2 (TGFβ2) and hydrogen peroxide (H2O2) on the epithelial–mesenchymal transition (EMT) of ARPE-19 cells, analyzing cell survival, cell migration by wound healing, and expression epithelial–mesenchymal transition markers at the protein and mRNA levels. Results For 24 h, human retinal pigment epithelial cells (ARPE-19) were cultured in standard conditions and subjected to various concentrations of TGFβ2, H2O2, and their combinations. According to a bioinformatics study, signaling pathways including hypoxia-inducible factor (HIF-1), forkhead box O (FoxO), phosphoinositide-3-kinase (PI3K-Akt), and transforming growth factors-β (TGFβ-signaling), both growth factors and oxidative stress-associated proteins play a significant role in the development of retinopathies. In vitro studies showed that oxidative stress induced by H2O2 and TGFβ2 enhances the epithelial–mesenchymal transition of ARPE-19 cells. Conclusions Hydrogen peroxide enhances the TGFβ2-mediated epithelial–mesenchymal transition of human retinal pigment epithelial cells, or at least in cell line ARPE-19 cells. Graphical abstracthttps://doi.org/10.1186/s43088-023-00421-5Epithelial–mesenchymal transitionHuman retinal pigment epithelial cellsTransforming growth factors-β2Hydrogen peroxideOxidative stressARPE-19 |
| spellingShingle | Brijesh Gelat Pooja Rathaur Pooja Malaviya Binita Patel Abhay R. Vasavada S. R. Kaid Johar Hydrogen peroxide enhances transforming growth factor beta-2 induced epithelial–mesenchymal transition of ARPE-19 cells Beni-Suef University Journal of Basic and Applied Sciences Epithelial–mesenchymal transition Human retinal pigment epithelial cells Transforming growth factors-β2 Hydrogen peroxide Oxidative stress ARPE-19 |
| title | Hydrogen peroxide enhances transforming growth factor beta-2 induced epithelial–mesenchymal transition of ARPE-19 cells |
| title_full | Hydrogen peroxide enhances transforming growth factor beta-2 induced epithelial–mesenchymal transition of ARPE-19 cells |
| title_fullStr | Hydrogen peroxide enhances transforming growth factor beta-2 induced epithelial–mesenchymal transition of ARPE-19 cells |
| title_full_unstemmed | Hydrogen peroxide enhances transforming growth factor beta-2 induced epithelial–mesenchymal transition of ARPE-19 cells |
| title_short | Hydrogen peroxide enhances transforming growth factor beta-2 induced epithelial–mesenchymal transition of ARPE-19 cells |
| title_sort | hydrogen peroxide enhances transforming growth factor beta 2 induced epithelial mesenchymal transition of arpe 19 cells |
| topic | Epithelial–mesenchymal transition Human retinal pigment epithelial cells Transforming growth factors-β2 Hydrogen peroxide Oxidative stress ARPE-19 |
| url | https://doi.org/10.1186/s43088-023-00421-5 |
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