In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells

Summary: Collecting-duct-derived renal epithelial cells switch from tubule to cyst formation; however, the cysts still form tubules after injury of the cyst-lining epithelium. Here, we provide a protocol that describes in vitro cyst growth with focus on glass-capillary-induced cyst wall injury to in...

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Bibliographic Details
Main Authors: Andre Kraus, Andrea Lüdke, Barbara Teschemacher, Kathrin Skoczynski, Bjoern Buchholz
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722007547