In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells
Summary: Collecting-duct-derived renal epithelial cells switch from tubule to cyst formation; however, the cysts still form tubules after injury of the cyst-lining epithelium. Here, we provide a protocol that describes in vitro cyst growth with focus on glass-capillary-induced cyst wall injury to in...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722007547 |
| _version_ | 1797946294270951424 |
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| author | Andre Kraus Andrea Lüdke Barbara Teschemacher Kathrin Skoczynski Bjoern Buchholz |
| author_facet | Andre Kraus Andrea Lüdke Barbara Teschemacher Kathrin Skoczynski Bjoern Buchholz |
| author_sort | Andre Kraus |
| collection | DOAJ |
| description | Summary: Collecting-duct-derived renal epithelial cells switch from tubule to cyst formation; however, the cysts still form tubules after injury of the cyst-lining epithelium. Here, we provide a protocol that describes in vitro cyst growth with focus on glass-capillary-induced cyst wall injury to induce tubule formation. We detail steps for the establishment of the in vitro cyst assay, followed by puncture of the cysts in the collagen matrix. We further describe live imaging and steps to analyze the tubule growth.For complete details on the use and execution of this protocol, please refer to Scholz et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-10T21:09:52Z |
| format | Article |
| id | doaj.art-655a278adf284e1caccaada8829e64da |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-10T21:09:52Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-655a278adf284e1caccaada8829e64da2023-01-21T04:21:39ZengElsevierSTAR Protocols2666-16672023-03-0141101874In vitro cyst puncture and injury-induced tubule formation using renal epithelial cellsAndre Kraus0Andrea Lüdke1Barbara Teschemacher2Kathrin Skoczynski3Bjoern Buchholz4Department of Nephrology and Hypertension, Friedrich-Alexander-University Erlangen-Nuernberg, Ulmenweg 18, 91054 Erlangen, Germany; Corresponding authorDepartment of Nephrology and Hypertension, Friedrich-Alexander-University Erlangen-Nuernberg, Ulmenweg 18, 91054 Erlangen, GermanyDepartment of Nephrology and Hypertension, Friedrich-Alexander-University Erlangen-Nuernberg, Ulmenweg 18, 91054 Erlangen, GermanyDepartment of Nephrology and Hypertension, Friedrich-Alexander-University Erlangen-Nuernberg, Ulmenweg 18, 91054 Erlangen, GermanyDepartment of Nephrology and Hypertension, Friedrich-Alexander-University Erlangen-Nuernberg, Ulmenweg 18, 91054 Erlangen, GermanySummary: Collecting-duct-derived renal epithelial cells switch from tubule to cyst formation; however, the cysts still form tubules after injury of the cyst-lining epithelium. Here, we provide a protocol that describes in vitro cyst growth with focus on glass-capillary-induced cyst wall injury to induce tubule formation. We detail steps for the establishment of the in vitro cyst assay, followed by puncture of the cysts in the collagen matrix. We further describe live imaging and steps to analyze the tubule growth.For complete details on the use and execution of this protocol, please refer to Scholz et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722007547Cell BiologyCell cultureCell-based AssaysMicroscopy |
| spellingShingle | Andre Kraus Andrea Lüdke Barbara Teschemacher Kathrin Skoczynski Bjoern Buchholz In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells STAR Protocols Cell Biology Cell culture Cell-based Assays Microscopy |
| title | In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells |
| title_full | In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells |
| title_fullStr | In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells |
| title_full_unstemmed | In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells |
| title_short | In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells |
| title_sort | in vitro cyst puncture and injury induced tubule formation using renal epithelial cells |
| topic | Cell Biology Cell culture Cell-based Assays Microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722007547 |
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