In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells
Summary: Collecting-duct-derived renal epithelial cells switch from tubule to cyst formation; however, the cysts still form tubules after injury of the cyst-lining epithelium. Here, we provide a protocol that describes in vitro cyst growth with focus on glass-capillary-induced cyst wall injury to in...
Main Authors: | Andre Kraus, Andrea Lüdke, Barbara Teschemacher, Kathrin Skoczynski, Bjoern Buchholz |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2023-03-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722007547 |
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