Caulerpa lentillifera improves ethanol-induced liver injury and modulates the gut microbiota in rats
Caulerpa lentillifera (CL), also called sea grape, is a type of edible green alga which was reported to have antioxidative and immunomodulatory potential. This study aimed to investigate the hepatoprotective effects of CL in a rat model of chronic ethanol exposure. Wistar rats were assigned to four...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Current Research in Food Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2665927123001144 |
| _version_ | 1797394820185980928 |
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| author | Kuan-Yu Lin Hsin-Yi Yang Suh-Ching Yang Ya-Ling Chen Y. Watanabe Jiun-Rong Chen |
| author_facet | Kuan-Yu Lin Hsin-Yi Yang Suh-Ching Yang Ya-Ling Chen Y. Watanabe Jiun-Rong Chen |
| author_sort | Kuan-Yu Lin |
| collection | DOAJ |
| description | Caulerpa lentillifera (CL), also called sea grape, is a type of edible green alga which was reported to have antioxidative and immunomodulatory potential. This study aimed to investigate the hepatoprotective effects of CL in a rat model of chronic ethanol exposure. Wistar rats were assigned to four groups and supplied with an isocaloric control liquid diet (group C), an ethanol liquid diet (group E), a control liquid diet supplemented with 5% CL (group CC), or an ethanol liquid diet supplemented with 5% CL (group EC) for a 12-week experimental period. Ethanol feeding induced steatosis, inflammation, and changes in the gut microbiota by the end of the study, whereas CL supplementation significantly improved liver injuries and decreased circulatory endotoxin levels. Moreover, we also found that CL reversed ethanol-induced elevation of hepatic toll-like receptor 4 (TLR4), MyD88 protein expression, the phosphorylated-nuclear factor (NF)-κB-to–NF–κB ratio, and proinflammatory cytokine concentrations. Additionally, CL also increased the abundance of Akkermansia and tight junction proteins and diminished the Firmicutes-to-Bacteroidetes ratio. Dietary CL inhibited the progression of alcoholic liver disease, and some of the possible mechanisms may be strengthening the intestinal barrier function, alleviating dysbiosis, and modulating the TLR4 pathway. |
| first_indexed | 2024-03-09T00:25:16Z |
| format | Article |
| id | doaj.art-656942697f734c7fb687239617a1fb90 |
| institution | Directory Open Access Journal |
| issn | 2665-9271 |
| language | English |
| last_indexed | 2024-03-09T00:25:16Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Food Science |
| spelling | doaj.art-656942697f734c7fb687239617a1fb902023-12-12T04:35:54ZengElsevierCurrent Research in Food Science2665-92712023-01-017100546Caulerpa lentillifera improves ethanol-induced liver injury and modulates the gut microbiota in ratsKuan-Yu Lin0Hsin-Yi Yang1Suh-Ching Yang2Ya-Ling Chen3Y. Watanabe4Jiun-Rong Chen5School of Nutrition and Health Sciences, Taipei Medical University, Taipei, TaiwanDepartment of Nutritional Science, Fu Jen Catholic University, New Taipei City, TaiwanSchool of Nutrition and Health Sciences, Taipei Medical University, Taipei, TaiwanSchool of Nutrition and Health Sciences, Taipei Medical University, Taipei, TaiwanGeneral Health Medical Center, Yokohama University of Pharmacy, Yokohama, JapanSchool of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan; Nutrition Research Center, Taipei Medical University Hospital, Taipei, Taiwan; Corresponding author. 250 Wu-Hsin Street, Taipei 110, Taiwan.Caulerpa lentillifera (CL), also called sea grape, is a type of edible green alga which was reported to have antioxidative and immunomodulatory potential. This study aimed to investigate the hepatoprotective effects of CL in a rat model of chronic ethanol exposure. Wistar rats were assigned to four groups and supplied with an isocaloric control liquid diet (group C), an ethanol liquid diet (group E), a control liquid diet supplemented with 5% CL (group CC), or an ethanol liquid diet supplemented with 5% CL (group EC) for a 12-week experimental period. Ethanol feeding induced steatosis, inflammation, and changes in the gut microbiota by the end of the study, whereas CL supplementation significantly improved liver injuries and decreased circulatory endotoxin levels. Moreover, we also found that CL reversed ethanol-induced elevation of hepatic toll-like receptor 4 (TLR4), MyD88 protein expression, the phosphorylated-nuclear factor (NF)-κB-to–NF–κB ratio, and proinflammatory cytokine concentrations. Additionally, CL also increased the abundance of Akkermansia and tight junction proteins and diminished the Firmicutes-to-Bacteroidetes ratio. Dietary CL inhibited the progression of alcoholic liver disease, and some of the possible mechanisms may be strengthening the intestinal barrier function, alleviating dysbiosis, and modulating the TLR4 pathway.http://www.sciencedirect.com/science/article/pii/S2665927123001144Alcoholic liver diseaseSea grapeInflammationMicrobiotaTLR4 |
| spellingShingle | Kuan-Yu Lin Hsin-Yi Yang Suh-Ching Yang Ya-Ling Chen Y. Watanabe Jiun-Rong Chen Caulerpa lentillifera improves ethanol-induced liver injury and modulates the gut microbiota in rats Current Research in Food Science Alcoholic liver disease Sea grape Inflammation Microbiota TLR4 |
| title | Caulerpa lentillifera improves ethanol-induced liver injury and modulates the gut microbiota in rats |
| title_full | Caulerpa lentillifera improves ethanol-induced liver injury and modulates the gut microbiota in rats |
| title_fullStr | Caulerpa lentillifera improves ethanol-induced liver injury and modulates the gut microbiota in rats |
| title_full_unstemmed | Caulerpa lentillifera improves ethanol-induced liver injury and modulates the gut microbiota in rats |
| title_short | Caulerpa lentillifera improves ethanol-induced liver injury and modulates the gut microbiota in rats |
| title_sort | caulerpa lentillifera improves ethanol induced liver injury and modulates the gut microbiota in rats |
| topic | Alcoholic liver disease Sea grape Inflammation Microbiota TLR4 |
| url | http://www.sciencedirect.com/science/article/pii/S2665927123001144 |
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