Evaluation of the Genmark ePlex® and QIAstat-Dx® respiratory pathogen panels in detecting bacterial targets in lower respiratory tract specimens

Abstract Background The ePlex® and QIAstat-Dx® respiratory pathogen panels detect multiple respiratory pathogens, mainly viruses but also Legionella pneumophila, Mycoplasma pneumoniae and Bordetella pertussis. The assays have been marketed for use in nasopharyngeal swab specimens. For diagnosing bac...

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Main Authors: Suzanne A. V. van Asten, Stefan A. Boers, Jolanda D. F. de Groot, R. Schuurman, Eric C. J. Claas
Format: Article
Language:English
Published: BMC 2021-08-01
Series:BMC Microbiology
Subjects:
Online Access:https://doi.org/10.1186/s12866-021-02289-w
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author Suzanne A. V. van Asten
Stefan A. Boers
Jolanda D. F. de Groot
R. Schuurman
Eric C. J. Claas
author_facet Suzanne A. V. van Asten
Stefan A. Boers
Jolanda D. F. de Groot
R. Schuurman
Eric C. J. Claas
author_sort Suzanne A. V. van Asten
collection DOAJ
description Abstract Background The ePlex® and QIAstat-Dx® respiratory pathogen panels detect multiple respiratory pathogens, mainly viruses but also Legionella pneumophila, Mycoplasma pneumoniae and Bordetella pertussis. The assays have been marketed for use in nasopharyngeal swab specimens. For diagnosing bacterial pneumonia, lower respiratory tract (LRT) specimens are indicated. Aim of this study was to evaluate the performance of these syndromic panels for these three bacterial targets in samples from the LRT. Fifty-six specimens were collected from our repositories, five negative samples and fifty-one samples which had been previously tested positive with the routine diagnostic real-time PCR assays for Legionella spp. (N = 20), Bordetella spp. (N = 16) or M. pneumoniae (N = 15). Results The QIAstat-Dx Respiratory Panel V2 (RP) assay detected all of the L. pneumophila and B. pertussis positive samples but only 11/15 (73.3 %) of the M. pneumoniae targets. The ePlex Respiratory Pathogen Panel (RPP) assay detected 10/14 (71.4 %) of the L. pneumophila targets, 8/12 (66.7 %) of the B. pertussis positive samples and 13/15 (86.7 %) of the M. pneumoniae targets. Conclusions No false-positive results were reported for all three bacterial pathogens by both assays. The clinical performance of both assays depended highly on the bacterial load in the sample and the type of specimen under investigation.
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spelling doaj.art-657bb70e115348039ca5054946a254832022-12-21T20:02:55ZengBMCBMC Microbiology1471-21802021-08-012111510.1186/s12866-021-02289-wEvaluation of the Genmark ePlex® and QIAstat-Dx® respiratory pathogen panels in detecting bacterial targets in lower respiratory tract specimensSuzanne A. V. van Asten0Stefan A. Boers1Jolanda D. F. de Groot2R. Schuurman3Eric C. J. Claas4Department of Medical Microbiology, Leiden University Medical CenterDepartment of Medical Microbiology, Leiden University Medical CenterDepartment of Medical Microbiology, University Medical Center UtrechtDepartment of Medical Microbiology, University Medical Center UtrechtDepartment of Medical Microbiology, Leiden University Medical CenterAbstract Background The ePlex® and QIAstat-Dx® respiratory pathogen panels detect multiple respiratory pathogens, mainly viruses but also Legionella pneumophila, Mycoplasma pneumoniae and Bordetella pertussis. The assays have been marketed for use in nasopharyngeal swab specimens. For diagnosing bacterial pneumonia, lower respiratory tract (LRT) specimens are indicated. Aim of this study was to evaluate the performance of these syndromic panels for these three bacterial targets in samples from the LRT. Fifty-six specimens were collected from our repositories, five negative samples and fifty-one samples which had been previously tested positive with the routine diagnostic real-time PCR assays for Legionella spp. (N = 20), Bordetella spp. (N = 16) or M. pneumoniae (N = 15). Results The QIAstat-Dx Respiratory Panel V2 (RP) assay detected all of the L. pneumophila and B. pertussis positive samples but only 11/15 (73.3 %) of the M. pneumoniae targets. The ePlex Respiratory Pathogen Panel (RPP) assay detected 10/14 (71.4 %) of the L. pneumophila targets, 8/12 (66.7 %) of the B. pertussis positive samples and 13/15 (86.7 %) of the M. pneumoniae targets. Conclusions No false-positive results were reported for all three bacterial pathogens by both assays. The clinical performance of both assays depended highly on the bacterial load in the sample and the type of specimen under investigation.https://doi.org/10.1186/s12866-021-02289-wePlexQIAstat-DxMolecular diagnosticsRespiratory tract infectionsSyndromic testing
spellingShingle Suzanne A. V. van Asten
Stefan A. Boers
Jolanda D. F. de Groot
R. Schuurman
Eric C. J. Claas
Evaluation of the Genmark ePlex® and QIAstat-Dx® respiratory pathogen panels in detecting bacterial targets in lower respiratory tract specimens
BMC Microbiology
ePlex
QIAstat-Dx
Molecular diagnostics
Respiratory tract infections
Syndromic testing
title Evaluation of the Genmark ePlex® and QIAstat-Dx® respiratory pathogen panels in detecting bacterial targets in lower respiratory tract specimens
title_full Evaluation of the Genmark ePlex® and QIAstat-Dx® respiratory pathogen panels in detecting bacterial targets in lower respiratory tract specimens
title_fullStr Evaluation of the Genmark ePlex® and QIAstat-Dx® respiratory pathogen panels in detecting bacterial targets in lower respiratory tract specimens
title_full_unstemmed Evaluation of the Genmark ePlex® and QIAstat-Dx® respiratory pathogen panels in detecting bacterial targets in lower respiratory tract specimens
title_short Evaluation of the Genmark ePlex® and QIAstat-Dx® respiratory pathogen panels in detecting bacterial targets in lower respiratory tract specimens
title_sort evaluation of the genmark eplex r and qiastat dx r respiratory pathogen panels in detecting bacterial targets in lower respiratory tract specimens
topic ePlex
QIAstat-Dx
Molecular diagnostics
Respiratory tract infections
Syndromic testing
url https://doi.org/10.1186/s12866-021-02289-w
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