Combination of Genetic Manipulation Improved <i>Saccharomycopsis fibuligera</i> α-Amylase Secretion by <i>Pichia pastoris</i>

Combination of Genetic Manipulation Improved <i>Saccharomycopsis fibuligera</i> α-Amylase Secretion by <i>Pichia pastoris</i>

This study assessed the combinations of genetic manipulation; signal peptide modification, gene dosage increment and co-expression of folding component, to increase Saccharomycopsis fibuligera R64 α-amylase (Sfamy) secretion in Pichia pastoris. Sfamy native signal peptide was replaced with modified...

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Bibliographic Details
Main Authors: Shabarni Gaffar, Dessy Natalia, Toto Subroto, Oo Suprijana, Soetijoso Soemitro
Format: Article
Language:English
Published: Department of Chemistry, Universitas Gadjah Mada 2019-04-01
Series:Indonesian Journal of Chemistry
Subjects:
Sfamy
signal peptide
gene dosage
folding
PDI
Pichia pastoris
Online Access:https://jurnal.ugm.ac.id/ijc/article/view/33140

Internet

https://jurnal.ugm.ac.id/ijc/article/view/33140

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