Stable Integration of Inducible SPLICS Reporters Enables Spatio-Temporal Analysis of Multiple Organelle Contact Sites upon Modulation of Cholesterol Traffic

Stable Integration of Inducible SPLICS Reporters Enables Spatio-Temporal Analysis of Multiple Organelle Contact Sites upon Modulation of Cholesterol Traffic

The study of organelle contact sites has received a great impulse due to increased interest in the understanding of their involvement in many disease conditions. Split-GFP-based contact sites (SPLICS) reporters emerged as essential tools to easily detect changes in a wide range of organelle contact...

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Bibliographic Details
Main Authors: Flavia Giamogante, Lucia Barazzuol, Elena Poggio, Marta Tromboni, Marisa Brini, Tito Calì
Format: Article
Language:English
Published: MDPI AG 2022-05-01
Series:Cells
Subjects:
SPLICS
organelle contact sites
split-GFP
stable cell lines
piggyBac system
Online Access:https://www.mdpi.com/2073-4409/11/10/1643

Internet

https://www.mdpi.com/2073-4409/11/10/1643

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