Exploring the interaction between extracellular matrix components in a 3D organoid disease model to replicate the pathophysiology of breast cancer
Abstract In vitro models are necessary to study the pathophysiology of the disease and the development of effective, tailored treatment methods owing to the complexity and heterogeneity of breast cancer and the large population affected by it. The cellular connections and tumor microenvironments obs...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | Journal of Experimental & Clinical Cancer Research |
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| Online Access: | https://doi.org/10.1186/s13046-023-02926-4 |
| _version_ | 1797387997419667456 |
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| author | Anamitra Bhattacharya Kamare Alam Nakka Sharmila Roy Kulwinder Kaur Santanu Kaity Velayutham Ravichandiran Subhadeep Roy |
| author_facet | Anamitra Bhattacharya Kamare Alam Nakka Sharmila Roy Kulwinder Kaur Santanu Kaity Velayutham Ravichandiran Subhadeep Roy |
| author_sort | Anamitra Bhattacharya |
| collection | DOAJ |
| description | Abstract In vitro models are necessary to study the pathophysiology of the disease and the development of effective, tailored treatment methods owing to the complexity and heterogeneity of breast cancer and the large population affected by it. The cellular connections and tumor microenvironments observed in vivo are often not recapitulated in conventional two-dimensional (2D) cell cultures. Therefore, developing 3D in vitro models that mimic the complex architecture and physiological circumstances of breast tumors is crucial for advancing our understanding of the illness. A 3D scaffold-free in vitro disease model mimics breast cancer pathophysiology by allowing cells to self-assemble/pattern into 3D structures, in contrast with other 3D models that rely on artificial scaffolds. It is possible that this model, whether applied to breast tumors using patient-derived primary cells (fibroblasts, endothelial cells, and cancer cells), can accurately replicate the observed heterogeneity. The complicated interactions between different cell types are modelled by integrating critical components of the tumor microenvironment, such as the extracellular matrix, vascular endothelial cells, and tumor growth factors. Tissue interactions, immune cell infiltration, and the effects of the milieu on drug resistance can be studied using this scaffold-free 3D model. The scaffold-free 3D in vitro disease model for mimicking tumor pathophysiology in breast cancer is a useful tool for studying the molecular basis of the disease, identifying new therapeutic targets, and evaluating treatment modalities. It provides a more physiologically appropriate high-throughput platform for screening large compound library in a 96–384 well format. We critically discussed the rapid development of personalized treatment strategies and accelerated drug screening platforms to close the gap between traditional 2D cell culture and in vivo investigations. Graphical Abstract |
| first_indexed | 2024-03-08T22:34:18Z |
| format | Article |
| id | doaj.art-658b694560054a75b7aa7d76c8ae1726 |
| institution | Directory Open Access Journal |
| issn | 1756-9966 |
| language | English |
| last_indexed | 2024-03-08T22:34:18Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj.art-658b694560054a75b7aa7d76c8ae17262023-12-17T12:33:38ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662023-12-0142112910.1186/s13046-023-02926-4Exploring the interaction between extracellular matrix components in a 3D organoid disease model to replicate the pathophysiology of breast cancerAnamitra Bhattacharya0Kamare Alam1Nakka Sharmila Roy2Kulwinder Kaur3Santanu Kaity4Velayutham Ravichandiran5Subhadeep Roy6Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and ResearchDepartment of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and ResearchDepartment of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and ResearchSchool of Pharmacy and Biomolecular Sciences, RCSI University of Medicine a Health SciencesDepartment of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and ResearchDepartment of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and ResearchDepartment of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and ResearchAbstract In vitro models are necessary to study the pathophysiology of the disease and the development of effective, tailored treatment methods owing to the complexity and heterogeneity of breast cancer and the large population affected by it. The cellular connections and tumor microenvironments observed in vivo are often not recapitulated in conventional two-dimensional (2D) cell cultures. Therefore, developing 3D in vitro models that mimic the complex architecture and physiological circumstances of breast tumors is crucial for advancing our understanding of the illness. A 3D scaffold-free in vitro disease model mimics breast cancer pathophysiology by allowing cells to self-assemble/pattern into 3D structures, in contrast with other 3D models that rely on artificial scaffolds. It is possible that this model, whether applied to breast tumors using patient-derived primary cells (fibroblasts, endothelial cells, and cancer cells), can accurately replicate the observed heterogeneity. The complicated interactions between different cell types are modelled by integrating critical components of the tumor microenvironment, such as the extracellular matrix, vascular endothelial cells, and tumor growth factors. Tissue interactions, immune cell infiltration, and the effects of the milieu on drug resistance can be studied using this scaffold-free 3D model. The scaffold-free 3D in vitro disease model for mimicking tumor pathophysiology in breast cancer is a useful tool for studying the molecular basis of the disease, identifying new therapeutic targets, and evaluating treatment modalities. It provides a more physiologically appropriate high-throughput platform for screening large compound library in a 96–384 well format. We critically discussed the rapid development of personalized treatment strategies and accelerated drug screening platforms to close the gap between traditional 2D cell culture and in vivo investigations. Graphical Abstracthttps://doi.org/10.1186/s13046-023-02926-43D organoidIn-vitro disease modelCell patterningTumor microenvironmentExtra cellular matrixCancer associated fibroblast |
| spellingShingle | Anamitra Bhattacharya Kamare Alam Nakka Sharmila Roy Kulwinder Kaur Santanu Kaity Velayutham Ravichandiran Subhadeep Roy Exploring the interaction between extracellular matrix components in a 3D organoid disease model to replicate the pathophysiology of breast cancer Journal of Experimental & Clinical Cancer Research 3D organoid In-vitro disease model Cell patterning Tumor microenvironment Extra cellular matrix Cancer associated fibroblast |
| title | Exploring the interaction between extracellular matrix components in a 3D organoid disease model to replicate the pathophysiology of breast cancer |
| title_full | Exploring the interaction between extracellular matrix components in a 3D organoid disease model to replicate the pathophysiology of breast cancer |
| title_fullStr | Exploring the interaction between extracellular matrix components in a 3D organoid disease model to replicate the pathophysiology of breast cancer |
| title_full_unstemmed | Exploring the interaction between extracellular matrix components in a 3D organoid disease model to replicate the pathophysiology of breast cancer |
| title_short | Exploring the interaction between extracellular matrix components in a 3D organoid disease model to replicate the pathophysiology of breast cancer |
| title_sort | exploring the interaction between extracellular matrix components in a 3d organoid disease model to replicate the pathophysiology of breast cancer |
| topic | 3D organoid In-vitro disease model Cell patterning Tumor microenvironment Extra cellular matrix Cancer associated fibroblast |
| url | https://doi.org/10.1186/s13046-023-02926-4 |
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