The effect of Amine modification on siRNA delivery of redox-responsive PEGylated amphiphilic micellar nanoparticles for triple negative breast cancer therapy
We developed a set of methoxypoly (ethyleneglycol)-co-poly(N3-ε-caprolactone) (mPEG-N3PCL)copolymers with different types of amine linkers and investigated the contribution of the introduced amino linker to the gene delivery efficiency of nanoparticles. The nanoparticles were crosslinked from the ca...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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University of Huddersfield Press
2022-11-01
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| Series: | British Journal of Pharmacy |
| Subjects: | |
| Online Access: | https://www.bjpharm.org.uk/article/id/1119/ |
| _version_ | 1797825315663249408 |
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| author | Fatemeh Mehradnia Cameron Alexander |
| author_facet | Fatemeh Mehradnia Cameron Alexander |
| author_sort | Fatemeh Mehradnia |
| collection | DOAJ |
| description | We developed a set of methoxypoly (ethyleneglycol)-co-poly(N3-ε-caprolactone) (mPEG-N3PCL)copolymers with different types of amine linkers and investigated the contribution of the introduced amino linker to the gene delivery efficiency of nanoparticles. The nanoparticles were crosslinked from the caprolactone regions with a redox-responsive linker. Formulation variables including Redox-crosslinking and N:P ratio were examined to obtain nanoparticles with optimal size and highest siRNA entrapment efficacy (EE). The nanoparticles were characterized by DLS, and nanodrop UV-vis spectroscopy.
Nanoparticle size, polydispersity index and siRNA entrapment efficacy were found to depend on the all the examined formulation variables specially the N:P ratio. The formulation with N:P ratio of 5 with56.13 nm in size and 92.24% EE was chosen as the optimal formulation.
Controllable size, loading efficiency and release pattern by redox-responsivity make this class of novel carriers a promising candidate for tumour targeted gene delivery applications. |
| first_indexed | 2024-03-13T10:52:17Z |
| format | Article |
| id | doaj.art-65bfc67a78db4a87b81a24f32e8401fe |
| institution | Directory Open Access Journal |
| issn | 2058-8356 |
| language | English |
| last_indexed | 2024-03-13T10:52:17Z |
| publishDate | 2022-11-01 |
| publisher | University of Huddersfield Press |
| record_format | Article |
| series | British Journal of Pharmacy |
| spelling | doaj.art-65bfc67a78db4a87b81a24f32e8401fe2023-05-17T10:41:59ZengUniversity of Huddersfield PressBritish Journal of Pharmacy2058-83562022-11-017210.5920/bjpharm.1119The effect of Amine modification on siRNA delivery of redox-responsive PEGylated amphiphilic micellar nanoparticles for triple negative breast cancer therapyFatemeh Mehradnia0Cameron Alexander1University of NottinghamUniversity of NottinghamWe developed a set of methoxypoly (ethyleneglycol)-co-poly(N3-ε-caprolactone) (mPEG-N3PCL)copolymers with different types of amine linkers and investigated the contribution of the introduced amino linker to the gene delivery efficiency of nanoparticles. The nanoparticles were crosslinked from the caprolactone regions with a redox-responsive linker. Formulation variables including Redox-crosslinking and N:P ratio were examined to obtain nanoparticles with optimal size and highest siRNA entrapment efficacy (EE). The nanoparticles were characterized by DLS, and nanodrop UV-vis spectroscopy. Nanoparticle size, polydispersity index and siRNA entrapment efficacy were found to depend on the all the examined formulation variables specially the N:P ratio. The formulation with N:P ratio of 5 with56.13 nm in size and 92.24% EE was chosen as the optimal formulation. Controllable size, loading efficiency and release pattern by redox-responsivity make this class of novel carriers a promising candidate for tumour targeted gene delivery applications. https://www.bjpharm.org.uk/article/id/1119/redox-responsive micellar nanoparticlessirna deliverytriple negative breast cancer |
| spellingShingle | Fatemeh Mehradnia Cameron Alexander The effect of Amine modification on siRNA delivery of redox-responsive PEGylated amphiphilic micellar nanoparticles for triple negative breast cancer therapy British Journal of Pharmacy redox-responsive micellar nanoparticles sirna delivery triple negative breast cancer |
| title | The effect of Amine modification on siRNA delivery of redox-responsive PEGylated amphiphilic micellar nanoparticles for triple negative breast cancer therapy |
| title_full | The effect of Amine modification on siRNA delivery of redox-responsive PEGylated amphiphilic micellar nanoparticles for triple negative breast cancer therapy |
| title_fullStr | The effect of Amine modification on siRNA delivery of redox-responsive PEGylated amphiphilic micellar nanoparticles for triple negative breast cancer therapy |
| title_full_unstemmed | The effect of Amine modification on siRNA delivery of redox-responsive PEGylated amphiphilic micellar nanoparticles for triple negative breast cancer therapy |
| title_short | The effect of Amine modification on siRNA delivery of redox-responsive PEGylated amphiphilic micellar nanoparticles for triple negative breast cancer therapy |
| title_sort | effect of amine modification on sirna delivery of redox responsive pegylated amphiphilic micellar nanoparticles for triple negative breast cancer therapy |
| topic | redox-responsive micellar nanoparticles sirna delivery triple negative breast cancer |
| url | https://www.bjpharm.org.uk/article/id/1119/ |
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