Effect of particle size on biological response by human monocyte-derived macrophages
Ultra-high molecular weight polyethylene (UHMWPE) wear particles from artificial joints induce osteolysis and the subsequent loosening of implants. Studies have reported that particles in the size range of 0.1–10 μm are the most biologically active in macrophage immune response. To develop prostheti...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-03-01
|
| Series: | Biosurface and Biotribology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405451816000040 |
| _version_ | 1828960056279826432 |
|---|---|
| author | H. Chikaura Y. Nakashima Y. Fujiwara Y. Komohara M. Takeya Y. Nakanishi |
| author_facet | H. Chikaura Y. Nakashima Y. Fujiwara Y. Komohara M. Takeya Y. Nakanishi |
| author_sort | H. Chikaura |
| collection | DOAJ |
| description | Ultra-high molecular weight polyethylene (UHMWPE) wear particles from artificial joints induce osteolysis and the subsequent loosening of implants. Studies have reported that particles in the size range of 0.1–10 μm are the most biologically active in macrophage immune response. To develop prosthetic joints with greater longevity and durability, it is crucial to understand the deleterious effects of wear particles. In this study, to evaluate the effects of particle size on the activities of human monocyte-derived macrophages (HMDMs), seven differently sized particles of polymethylmethacrylate (PMMA), in the range of 0.1–20 μm, were prepared. Viability and the secretion of cytokines were evaluated after phagocytosis of each size particles by HMDMs. Differences in the viability of HMDMs after phagocytosis of particles sized 0.16–9.6 μm were statistically significant. Proinflammatory cytokine production of both tumor necrosis factor-α and interleukin-6 by HMDMs was strongly induced by 0.8 μm PMMA particles. Consistent with the fact that macrophages are known to respond to pathogens measuring approximately 1.0 μm in size, in this study, PMMA particles measuring 0.8 μm in size induced an immune response. This work provides fundamental data for the designing of surface profiles of prosthetic joints, Which may expect the lower incidence of immune response. |
| first_indexed | 2024-12-14T09:19:04Z |
| format | Article |
| id | doaj.art-65cd9a367ad04d159f44b2db5246777a |
| institution | Directory Open Access Journal |
| issn | 2405-4518 |
| language | English |
| last_indexed | 2024-12-14T09:19:04Z |
| publishDate | 2016-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biosurface and Biotribology |
| spelling | doaj.art-65cd9a367ad04d159f44b2db5246777a2022-12-21T23:08:21ZengWileyBiosurface and Biotribology2405-45182016-03-0121182510.1016/j.bsbt.2016.02.003Effect of particle size on biological response by human monocyte-derived macrophagesH. Chikaura0Y. Nakashima1Y. Fujiwara2Y. Komohara3M. Takeya4Y. Nakanishi5Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, JapanGraduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, JapanGraduate School of Medical Sciences, Kumamoto University, Kumamoto 860-8555, JapanGraduate School of Medical Sciences, Kumamoto University, Kumamoto 860-8555, JapanGraduate School of Medical Sciences, Kumamoto University, Kumamoto 860-8555, JapanGraduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, JapanUltra-high molecular weight polyethylene (UHMWPE) wear particles from artificial joints induce osteolysis and the subsequent loosening of implants. Studies have reported that particles in the size range of 0.1–10 μm are the most biologically active in macrophage immune response. To develop prosthetic joints with greater longevity and durability, it is crucial to understand the deleterious effects of wear particles. In this study, to evaluate the effects of particle size on the activities of human monocyte-derived macrophages (HMDMs), seven differently sized particles of polymethylmethacrylate (PMMA), in the range of 0.1–20 μm, were prepared. Viability and the secretion of cytokines were evaluated after phagocytosis of each size particles by HMDMs. Differences in the viability of HMDMs after phagocytosis of particles sized 0.16–9.6 μm were statistically significant. Proinflammatory cytokine production of both tumor necrosis factor-α and interleukin-6 by HMDMs was strongly induced by 0.8 μm PMMA particles. Consistent with the fact that macrophages are known to respond to pathogens measuring approximately 1.0 μm in size, in this study, PMMA particles measuring 0.8 μm in size induced an immune response. This work provides fundamental data for the designing of surface profiles of prosthetic joints, Which may expect the lower incidence of immune response.http://www.sciencedirect.com/science/article/pii/S2405451816000040Particle sizeBiological responseArtificial jointMacrophageCytokines |
| spellingShingle | H. Chikaura Y. Nakashima Y. Fujiwara Y. Komohara M. Takeya Y. Nakanishi Effect of particle size on biological response by human monocyte-derived macrophages Biosurface and Biotribology Particle size Biological response Artificial joint Macrophage Cytokines |
| title | Effect of particle size on biological response by human monocyte-derived macrophages |
| title_full | Effect of particle size on biological response by human monocyte-derived macrophages |
| title_fullStr | Effect of particle size on biological response by human monocyte-derived macrophages |
| title_full_unstemmed | Effect of particle size on biological response by human monocyte-derived macrophages |
| title_short | Effect of particle size on biological response by human monocyte-derived macrophages |
| title_sort | effect of particle size on biological response by human monocyte derived macrophages |
| topic | Particle size Biological response Artificial joint Macrophage Cytokines |
| url | http://www.sciencedirect.com/science/article/pii/S2405451816000040 |
| work_keys_str_mv | AT hchikaura effectofparticlesizeonbiologicalresponsebyhumanmonocytederivedmacrophages AT ynakashima effectofparticlesizeonbiologicalresponsebyhumanmonocytederivedmacrophages AT yfujiwara effectofparticlesizeonbiologicalresponsebyhumanmonocytederivedmacrophages AT ykomohara effectofparticlesizeonbiologicalresponsebyhumanmonocytederivedmacrophages AT mtakeya effectofparticlesizeonbiologicalresponsebyhumanmonocytederivedmacrophages AT ynakanishi effectofparticlesizeonbiologicalresponsebyhumanmonocytederivedmacrophages |