SOME ASPECTS OF PRIMER DESIGN FOR REAL TIME PCR WITH SYBR Green AS A DYE
This work considers the sequence of steps for designing primers for real time qPCR analysis with SYBR® Green as fluorescent dye. For primer design, we used Primer- Blast software. As an example, we design primers to fum6 gene, a member of fumonisin biosynthetic gene cluster. The importance of a bala...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Technical University of Moldova
2020-12-01
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| Series: | Journal of Engineering Science (Chişinău) |
| Subjects: | |
| Online Access: | https://jes.utm.md/wp-content/uploads/sites/20/2020/12/JES-2020-4_191-196.pdf |