HIF-1α siRNA Leads to Apoptosis of Pancreatic Cancer Cells under Hypoxic Conditions
OBJECTIVE To explore the role of hypoxic inducible factor-1α (HIF-1 α ) in the proliferation and apoptosis of pancreatic cancer cells under hypoxic conditions. METHODS A cassette encoding small interference RNA (siRNA) targeting HIF-1 α mediated by recombinant adeno-associated virus (rAAV) was co...
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| Format: | Article |
| Language: | English |
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China Anti-Cancer Association
2009-02-01
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| Series: | Cancer Biology & Medicine |
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| Online Access: | http://www.cancerbiomed.org/index.php/cocr/article/view/167 |
| _version_ | 1818981098959405056 |
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| author | Chuangui CHEN, Jianqiu CHEN, Jinjin SUN |
| author_facet | Chuangui CHEN, Jianqiu CHEN, Jinjin SUN |
| author_sort | Chuangui CHEN, Jianqiu CHEN, Jinjin SUN |
| collection | DOAJ |
| description | OBJECTIVE To explore the role of hypoxic inducible factor-1α (HIF-1 α ) in the proliferation and apoptosis of pancreatic cancer cells under hypoxic conditions. METHODS A cassette encoding small interference RNA (siRNA) targeting HIF-1 α mediated by recombinant adeno-associated virus (rAAV) was constructed, giving rAAV-siHIF. rAAV-siHIF or rAAV-hrGFP was transfected into exponentially growing MiaPaCa2 cells under hypoxic conditions. Then, the expression of HIF-1α mRNA and protein, the proliferation and apoptosis of MiaPaCa2 cells were examined, using real-time PCR, Western Blot, MTT and TUNEL, respectively. RESULTS Under hypoxic conditions, rAAV-siHIF inhibited the expression of HIF-1 α mRNA and protein in MiaPaCa2 cells. At the same time, rAAV-siHIF decreased MiaPaCa2 cell proliferation and induced apoptosis. However, rAAV-hrGFP had no effect on the expression of HIF-1 α as well as the proliferation and apoptosis of MiaPaCa2 cells under hypoxic conditions. CONCLUSION Under hypoxic conditions, HIF-1α plays a key role in the proliferation of MiaPaCa2 cells, and inhibition of HIF-1α expression can lead to MiaPaCa2 cell apoptosis. |
| first_indexed | 2024-12-20T17:25:56Z |
| format | Article |
| id | doaj.art-662d3095721a4a888136da972cd36a16 |
| institution | Directory Open Access Journal |
| issn | 2095-3941 |
| language | English |
| last_indexed | 2024-12-20T17:25:56Z |
| publishDate | 2009-02-01 |
| publisher | China Anti-Cancer Association |
| record_format | Article |
| series | Cancer Biology & Medicine |
| spelling | doaj.art-662d3095721a4a888136da972cd36a162022-12-21T19:31:35ZengChina Anti-Cancer AssociationCancer Biology & Medicine2095-39412009-02-0161101510.1007/s11805-009-0010-5HIF-1α siRNA Leads to Apoptosis of Pancreatic Cancer Cells under Hypoxic ConditionsChuangui CHEN, Jianqiu CHEN, Jinjin SUNOBJECTIVE To explore the role of hypoxic inducible factor-1α (HIF-1 α ) in the proliferation and apoptosis of pancreatic cancer cells under hypoxic conditions. METHODS A cassette encoding small interference RNA (siRNA) targeting HIF-1 α mediated by recombinant adeno-associated virus (rAAV) was constructed, giving rAAV-siHIF. rAAV-siHIF or rAAV-hrGFP was transfected into exponentially growing MiaPaCa2 cells under hypoxic conditions. Then, the expression of HIF-1α mRNA and protein, the proliferation and apoptosis of MiaPaCa2 cells were examined, using real-time PCR, Western Blot, MTT and TUNEL, respectively. RESULTS Under hypoxic conditions, rAAV-siHIF inhibited the expression of HIF-1 α mRNA and protein in MiaPaCa2 cells. At the same time, rAAV-siHIF decreased MiaPaCa2 cell proliferation and induced apoptosis. However, rAAV-hrGFP had no effect on the expression of HIF-1 α as well as the proliferation and apoptosis of MiaPaCa2 cells under hypoxic conditions. CONCLUSION Under hypoxic conditions, HIF-1α plays a key role in the proliferation of MiaPaCa2 cells, and inhibition of HIF-1α expression can lead to MiaPaCa2 cell apoptosis.http://www.cancerbiomed.org/index.php/cocr/article/view/167recombinant adeno-associated virus (rAAV)hypoxia inducible factor (HIF)small interference RNA (siRNA)proliferationapoptosis. |
| spellingShingle | Chuangui CHEN, Jianqiu CHEN, Jinjin SUN HIF-1α siRNA Leads to Apoptosis of Pancreatic Cancer Cells under Hypoxic Conditions Cancer Biology & Medicine recombinant adeno-associated virus (rAAV) hypoxia inducible factor (HIF) small interference RNA (siRNA) proliferation apoptosis. |
| title | HIF-1α siRNA Leads to Apoptosis of Pancreatic Cancer Cells under Hypoxic Conditions |
| title_full | HIF-1α siRNA Leads to Apoptosis of Pancreatic Cancer Cells under Hypoxic Conditions |
| title_fullStr | HIF-1α siRNA Leads to Apoptosis of Pancreatic Cancer Cells under Hypoxic Conditions |
| title_full_unstemmed | HIF-1α siRNA Leads to Apoptosis of Pancreatic Cancer Cells under Hypoxic Conditions |
| title_short | HIF-1α siRNA Leads to Apoptosis of Pancreatic Cancer Cells under Hypoxic Conditions |
| title_sort | hif 1α sirna leads to apoptosis of pancreatic cancer cells under hypoxic conditions |
| topic | recombinant adeno-associated virus (rAAV) hypoxia inducible factor (HIF) small interference RNA (siRNA) proliferation apoptosis. |
| url | http://www.cancerbiomed.org/index.php/cocr/article/view/167 |
| work_keys_str_mv | AT chuanguichenjianqiuchenjinjinsun hif1asirnaleadstoapoptosisofpancreaticcancercellsunderhypoxicconditions |