Application of LC-MS/MS Coupled with Various Digestion Methods for the Identification of Porcine Gelatin Markers in Confectionery Matrices
Gelatin is a high-risk ingredient in terms of its halal status. Liquid chromatography combined with mass spectrometry (LC-MS/MS) was used to identify the source of gelatin based on marker peptides and proved to deliver higher reliability than other methods. However, the digestion method is essential...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Halal Center UIN Sunan Gunung Djati Bandung
2023-08-01
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| Series: | Indonesian Journal of Halal Research |
| Subjects: | |
| Online Access: | https://journal.uinsgd.ac.id/index.php/ijhar/article/view/21191 |
| _version_ | 1827762369455980544 |
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| author | Kifayati Rosiyanti Dewi Feri Kusnandar Nancy Dewi Yuliana Maya Ismayati Nissa Nurfajrin Solihat Handy Riantana Heryani Heryani |
| author_facet | Kifayati Rosiyanti Dewi Feri Kusnandar Nancy Dewi Yuliana Maya Ismayati Nissa Nurfajrin Solihat Handy Riantana Heryani Heryani |
| author_sort | Kifayati Rosiyanti Dewi |
| collection | DOAJ |
| description | Gelatin is a high-risk ingredient in terms of its halal status. Liquid chromatography combined with mass spectrometry (LC-MS/MS) was used to identify the source of gelatin based on marker peptides and proved to deliver higher reliability than other methods. However, the digestion method is essential before LC-MS/MS analysis. This research evaluated different digestion methods against selected porcine gelatin marker peptides and assessed LC-MS/MS sensitivity through adulteration experiments in various mixed matrices. The study involved three digestion methods (conventional, microwave, and ultrasound) before LC-MS/MS analysis to determine the most effective method for detecting marker peptide targets from porcine gelatin. The appropriate method was applied to isolate porcine gelatin peptides in the matrices of bovine gelatin and confectionery products (lozenges, marshmallows, and soft candy) at concentrations of 0.01, 0.1, and 1% (w/w). Relative detection limit values were determined. The results showed that conventional digestion treatment yielded a higher marker peptide detection rate than microwave and ultrasound digestion. Meanwhile, the detection limit of porcine gelatin in bovine gelatin ranged from 0.09 to 0.89%, depending on the marker peptide used, and could be significantly detected at a concentration of 1% in the confectionery product. The marker peptide TGQPGAVGPAGIR exhibited the highest stability, as it was detectable at the lowest concentration across all mixed matrices. The LC-MS/MS method has been proven to afford sensitive results and has the potential to serve as an alternative for detection of halal status. |
| first_indexed | 2024-03-11T10:29:43Z |
| format | Article |
| id | doaj.art-664b830e323743ddb81dbed80778da91 |
| institution | Directory Open Access Journal |
| issn | 2656-3754 2657-0165 |
| language | English |
| last_indexed | 2024-03-11T10:29:43Z |
| publishDate | 2023-08-01 |
| publisher | Halal Center UIN Sunan Gunung Djati Bandung |
| record_format | Article |
| series | Indonesian Journal of Halal Research |
| spelling | doaj.art-664b830e323743ddb81dbed80778da912023-11-15T04:15:37ZengHalal Center UIN Sunan Gunung Djati BandungIndonesian Journal of Halal Research2656-37542657-01652023-08-0152536610.15575/ijhar.v5i2.211917896Application of LC-MS/MS Coupled with Various Digestion Methods for the Identification of Porcine Gelatin Markers in Confectionery MatricesKifayati Rosiyanti Dewi0Feri Kusnandar1Nancy Dewi Yuliana2Maya Ismayati3Nissa Nurfajrin Solihat4Handy Riantana5Heryani Heryani6IPB University & National Research and Innovation Agency (BRIN)IPB University & Halal Science Center IPB UniversityIPB University & Halal Science Center IPB UniversityNational Research and Innovation Agency (BRIN)National Research and Innovation Agency (BRIN)National Research and Innovation Agency (BRIN) & Khon Kaen UniversityThe Assessment Institute for Foods, Drugs and Cosmetics, the Indonesian Council of Ulama (LPPOM MUI)Gelatin is a high-risk ingredient in terms of its halal status. Liquid chromatography combined with mass spectrometry (LC-MS/MS) was used to identify the source of gelatin based on marker peptides and proved to deliver higher reliability than other methods. However, the digestion method is essential before LC-MS/MS analysis. This research evaluated different digestion methods against selected porcine gelatin marker peptides and assessed LC-MS/MS sensitivity through adulteration experiments in various mixed matrices. The study involved three digestion methods (conventional, microwave, and ultrasound) before LC-MS/MS analysis to determine the most effective method for detecting marker peptide targets from porcine gelatin. The appropriate method was applied to isolate porcine gelatin peptides in the matrices of bovine gelatin and confectionery products (lozenges, marshmallows, and soft candy) at concentrations of 0.01, 0.1, and 1% (w/w). Relative detection limit values were determined. The results showed that conventional digestion treatment yielded a higher marker peptide detection rate than microwave and ultrasound digestion. Meanwhile, the detection limit of porcine gelatin in bovine gelatin ranged from 0.09 to 0.89%, depending on the marker peptide used, and could be significantly detected at a concentration of 1% in the confectionery product. The marker peptide TGQPGAVGPAGIR exhibited the highest stability, as it was detectable at the lowest concentration across all mixed matrices. The LC-MS/MS method has been proven to afford sensitive results and has the potential to serve as an alternative for detection of halal status.https://journal.uinsgd.ac.id/index.php/ijhar/article/view/21191confectionerygelatinlc-ms/msporcine |
| spellingShingle | Kifayati Rosiyanti Dewi Feri Kusnandar Nancy Dewi Yuliana Maya Ismayati Nissa Nurfajrin Solihat Handy Riantana Heryani Heryani Application of LC-MS/MS Coupled with Various Digestion Methods for the Identification of Porcine Gelatin Markers in Confectionery Matrices Indonesian Journal of Halal Research confectionery gelatin lc-ms/ms porcine |
| title | Application of LC-MS/MS Coupled with Various Digestion Methods for the Identification of Porcine Gelatin Markers in Confectionery Matrices |
| title_full | Application of LC-MS/MS Coupled with Various Digestion Methods for the Identification of Porcine Gelatin Markers in Confectionery Matrices |
| title_fullStr | Application of LC-MS/MS Coupled with Various Digestion Methods for the Identification of Porcine Gelatin Markers in Confectionery Matrices |
| title_full_unstemmed | Application of LC-MS/MS Coupled with Various Digestion Methods for the Identification of Porcine Gelatin Markers in Confectionery Matrices |
| title_short | Application of LC-MS/MS Coupled with Various Digestion Methods for the Identification of Porcine Gelatin Markers in Confectionery Matrices |
| title_sort | application of lc ms ms coupled with various digestion methods for the identification of porcine gelatin markers in confectionery matrices |
| topic | confectionery gelatin lc-ms/ms porcine |
| url | https://journal.uinsgd.ac.id/index.php/ijhar/article/view/21191 |
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