Optimization, antioxidant activity and bile salts adsorption capacity of the aqueous enzymatic extract from rice bran
The optimum extraction conditions of the aqueous enzymatic extract from rice bran (AEERB) were evaluated with respect to γ-oryzanol content and ferric reducing/antioxidant power (FRAP) activity by using response surface methodology. The results showed that the effect of the extraction temperature on...
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Czech Academy of Agricultural Sciences
2018-08-01
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Series: | Czech Journal of Food Sciences |
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Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-201804-0010_optimization-antioxidant-activity-and-bile-salts-adsorption-capacity-of-the-aqueous-enzymatic-extract-from-ric.php |
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author | Yuxin WANG Guoping YU Xiaodan ZANG Fei YE |
author_facet | Yuxin WANG Guoping YU Xiaodan ZANG Fei YE |
author_sort | Yuxin WANG |
collection | DOAJ |
description | The optimum extraction conditions of the aqueous enzymatic extract from rice bran (AEERB) were evaluated with respect to γ-oryzanol content and ferric reducing/antioxidant power (FRAP) activity by using response surface methodology. The results showed that the effect of the extraction temperature on γ-oryzanol content and FRAP activity was the most significant. The optimal conditions were determined as follows: Trypsin, 453 U/g at 56°C and pH 7.9 for 2 h at a liquid/solid ratio of 7 : 1. The corresponding γ-oryzanol content and FRAP activity were 0.1749 mg/g and 0.5043 mmol FeSO4/g, respectively. The antioxidant activities of AEERB were comparable with 2,6-di-tert-buty-4-methylphenol (BHT) or ascorbic acid (AA) at the high concentration. Furthermore, the binding capacities of AEERB to sodium taurocholate and sodium deoxycholate were 24.96 and 13.63 µmol/100 mg, respectively. The results indicated that AEERB is a promising method for developing and applying a value-added RB product. |
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issn | 1212-1800 1805-9317 |
language | English |
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spelling | doaj.art-66502a0df74947d89c881df4f660bf4a2023-02-23T03:28:31ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172018-08-0136433834810.17221/369/2017-CJFScjf-201804-0010Optimization, antioxidant activity and bile salts adsorption capacity of the aqueous enzymatic extract from rice branYuxin WANG0Guoping YU1Xiaodan ZANG2Fei YECollege of Science, Northeast Agricultural University, Harbin, P.R. ChinaCollege of Science, Northeast Agricultural University, Harbin, P.R. ChinaCollege of Science, Northeast Agricultural University, Harbin, P.R. ChinaThe optimum extraction conditions of the aqueous enzymatic extract from rice bran (AEERB) were evaluated with respect to γ-oryzanol content and ferric reducing/antioxidant power (FRAP) activity by using response surface methodology. The results showed that the effect of the extraction temperature on γ-oryzanol content and FRAP activity was the most significant. The optimal conditions were determined as follows: Trypsin, 453 U/g at 56°C and pH 7.9 for 2 h at a liquid/solid ratio of 7 : 1. The corresponding γ-oryzanol content and FRAP activity were 0.1749 mg/g and 0.5043 mmol FeSO4/g, respectively. The antioxidant activities of AEERB were comparable with 2,6-di-tert-buty-4-methylphenol (BHT) or ascorbic acid (AA) at the high concentration. Furthermore, the binding capacities of AEERB to sodium taurocholate and sodium deoxycholate were 24.96 and 13.63 µmol/100 mg, respectively. The results indicated that AEERB is a promising method for developing and applying a value-added RB product.https://cjfs.agriculturejournals.cz/artkey/cjf-201804-0010_optimization-antioxidant-activity-and-bile-salts-adsorption-capacity-of-the-aqueous-enzymatic-extract-from-ric.phpantioxidativeaqueous enzymatic extractshypolipidemicrice branoptimization |
spellingShingle | Yuxin WANG Guoping YU Xiaodan ZANG Fei YE Optimization, antioxidant activity and bile salts adsorption capacity of the aqueous enzymatic extract from rice bran Czech Journal of Food Sciences antioxidative aqueous enzymatic extracts hypolipidemic rice bran optimization |
title | Optimization, antioxidant activity and bile salts adsorption capacity of the aqueous enzymatic extract from rice bran |
title_full | Optimization, antioxidant activity and bile salts adsorption capacity of the aqueous enzymatic extract from rice bran |
title_fullStr | Optimization, antioxidant activity and bile salts adsorption capacity of the aqueous enzymatic extract from rice bran |
title_full_unstemmed | Optimization, antioxidant activity and bile salts adsorption capacity of the aqueous enzymatic extract from rice bran |
title_short | Optimization, antioxidant activity and bile salts adsorption capacity of the aqueous enzymatic extract from rice bran |
title_sort | optimization antioxidant activity and bile salts adsorption capacity of the aqueous enzymatic extract from rice bran |
topic | antioxidative aqueous enzymatic extracts hypolipidemic rice bran optimization |
url | https://cjfs.agriculturejournals.cz/artkey/cjf-201804-0010_optimization-antioxidant-activity-and-bile-salts-adsorption-capacity-of-the-aqueous-enzymatic-extract-from-ric.php |
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