Optimization, antioxidant activity and bile salts adsorption capacity of the aqueous enzymatic extract from rice bran

The optimum extraction conditions of the aqueous enzymatic extract from rice bran (AEERB) were evaluated with respect to γ-oryzanol content and ferric reducing/antioxidant power (FRAP) activity by using response surface methodology. The results showed that the effect of the extraction temperature on...

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Main Authors: Yuxin WANG, Guoping YU, Xiaodan ZANG, Fei YE
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2018-08-01
Series:Czech Journal of Food Sciences
Subjects:
Online Access:https://cjfs.agriculturejournals.cz/artkey/cjf-201804-0010_optimization-antioxidant-activity-and-bile-salts-adsorption-capacity-of-the-aqueous-enzymatic-extract-from-ric.php
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author Yuxin WANG
Guoping YU
Xiaodan ZANG
Fei YE
author_facet Yuxin WANG
Guoping YU
Xiaodan ZANG
Fei YE
author_sort Yuxin WANG
collection DOAJ
description The optimum extraction conditions of the aqueous enzymatic extract from rice bran (AEERB) were evaluated with respect to γ-oryzanol content and ferric reducing/antioxidant power (FRAP) activity by using response surface methodology. The results showed that the effect of the extraction temperature on γ-oryzanol content and FRAP activity was the most significant. The optimal conditions were determined as follows: Trypsin, 453 U/g at 56°C and pH 7.9 for 2 h at a liquid/solid ratio of 7 : 1. The corresponding γ-oryzanol content and FRAP activity were 0.1749 mg/g and 0.5043 mmol FeSO4/g, respectively. The antioxidant activities of AEERB were comparable with 2,6-di-tert-buty-4-methylphenol (BHT) or ascorbic acid (AA) at the high concentration. Furthermore, the binding capacities of AEERB to sodium taurocholate and sodium deoxycholate were 24.96 and 13.63 µmol/100 mg, respectively. The results indicated that AEERB is a promising method for developing and applying a value-added RB product.
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spelling doaj.art-66502a0df74947d89c881df4f660bf4a2023-02-23T03:28:31ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172018-08-0136433834810.17221/369/2017-CJFScjf-201804-0010Optimization, antioxidant activity and bile salts adsorption capacity of the aqueous enzymatic extract from rice branYuxin WANG0Guoping YU1Xiaodan ZANG2Fei YECollege of Science, Northeast Agricultural University, Harbin, P.R. ChinaCollege of Science, Northeast Agricultural University, Harbin, P.R. ChinaCollege of Science, Northeast Agricultural University, Harbin, P.R. ChinaThe optimum extraction conditions of the aqueous enzymatic extract from rice bran (AEERB) were evaluated with respect to γ-oryzanol content and ferric reducing/antioxidant power (FRAP) activity by using response surface methodology. The results showed that the effect of the extraction temperature on γ-oryzanol content and FRAP activity was the most significant. The optimal conditions were determined as follows: Trypsin, 453 U/g at 56°C and pH 7.9 for 2 h at a liquid/solid ratio of 7 : 1. The corresponding γ-oryzanol content and FRAP activity were 0.1749 mg/g and 0.5043 mmol FeSO4/g, respectively. The antioxidant activities of AEERB were comparable with 2,6-di-tert-buty-4-methylphenol (BHT) or ascorbic acid (AA) at the high concentration. Furthermore, the binding capacities of AEERB to sodium taurocholate and sodium deoxycholate were 24.96 and 13.63 µmol/100 mg, respectively. The results indicated that AEERB is a promising method for developing and applying a value-added RB product.https://cjfs.agriculturejournals.cz/artkey/cjf-201804-0010_optimization-antioxidant-activity-and-bile-salts-adsorption-capacity-of-the-aqueous-enzymatic-extract-from-ric.phpantioxidativeaqueous enzymatic extractshypolipidemicrice branoptimization
spellingShingle Yuxin WANG
Guoping YU
Xiaodan ZANG
Fei YE
Optimization, antioxidant activity and bile salts adsorption capacity of the aqueous enzymatic extract from rice bran
Czech Journal of Food Sciences
antioxidative
aqueous enzymatic extracts
hypolipidemic
rice bran
optimization
title Optimization, antioxidant activity and bile salts adsorption capacity of the aqueous enzymatic extract from rice bran
title_full Optimization, antioxidant activity and bile salts adsorption capacity of the aqueous enzymatic extract from rice bran
title_fullStr Optimization, antioxidant activity and bile salts adsorption capacity of the aqueous enzymatic extract from rice bran
title_full_unstemmed Optimization, antioxidant activity and bile salts adsorption capacity of the aqueous enzymatic extract from rice bran
title_short Optimization, antioxidant activity and bile salts adsorption capacity of the aqueous enzymatic extract from rice bran
title_sort optimization antioxidant activity and bile salts adsorption capacity of the aqueous enzymatic extract from rice bran
topic antioxidative
aqueous enzymatic extracts
hypolipidemic
rice bran
optimization
url https://cjfs.agriculturejournals.cz/artkey/cjf-201804-0010_optimization-antioxidant-activity-and-bile-salts-adsorption-capacity-of-the-aqueous-enzymatic-extract-from-ric.php
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AT guopingyu optimizationantioxidantactivityandbilesaltsadsorptioncapacityoftheaqueousenzymaticextractfromricebran
AT xiaodanzang optimizationantioxidantactivityandbilesaltsadsorptioncapacityoftheaqueousenzymaticextractfromricebran
AT feiye optimizationantioxidantactivityandbilesaltsadsorptioncapacityoftheaqueousenzymaticextractfromricebran