Translational regulation of periplasmic folding assistants and proteases as a valuable strategy to improve production of translocated recombinant proteins in Escherichia coli

Translational regulation of periplasmic folding assistants and proteases as a valuable strategy to improve production of translocated recombinant proteins in Escherichia coli

Abstract Background Advantages of translocation of recombinant proteins to the periplasm in Escherichia coli include simplified downstream processing, and improved folding and in vivo activity of the target protein. There are, however, problems encountered in the periplasmic production that can be a...

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Bibliographic Details
Main Authors: Agnieszka Gawin, Helga Ertesvåg, Sine Alise Hartvigsen Hansen, Jostein Malmo, Trygve Brautaset
Format: Article
Language:English
Published: BMC 2020-05-01
Series:BMC Biotechnology
Subjects:
Periplasmic translocation
Recombinant protein production
Translation initiation rate
Ribosome binding site
Genome editing
Online Access:http://link.springer.com/article/10.1186/s12896-020-00615-0

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http://link.springer.com/article/10.1186/s12896-020-00615-0

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