Global assessment of genomic regions required for growth in Mycobacterium tuberculosis.
Identifying genomic elements required for viability is central to our understanding of the basic physiology of bacterial pathogens. Recently, the combination of high-density mutagenesis and deep sequencing has allowed for the identification of required and conditionally required genes in many bacter...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-09-01
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| Series: | PLoS Pathogens |
| Online Access: | http://europepmc.org/articles/PMC3460630?pdf=render |
| _version_ | 1818335294904074240 |
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| author | Yanjia J Zhang Thomas R Ioerger Curtis Huttenhower Jarukit E Long Christopher M Sassetti James C Sacchettini Eric J Rubin |
| author_facet | Yanjia J Zhang Thomas R Ioerger Curtis Huttenhower Jarukit E Long Christopher M Sassetti James C Sacchettini Eric J Rubin |
| author_sort | Yanjia J Zhang |
| collection | DOAJ |
| description | Identifying genomic elements required for viability is central to our understanding of the basic physiology of bacterial pathogens. Recently, the combination of high-density mutagenesis and deep sequencing has allowed for the identification of required and conditionally required genes in many bacteria. Genes, however, make up only a part of the complex genomes of important bacterial pathogens. Here, we use an unbiased analysis to comprehensively identify genomic regions, including genes, domains, and intergenic elements, required for the optimal growth of Mycobacterium tuberculosis, a major global health pathogen. We found that several proteins jointly contain both domains required for optimal growth and domains that are dispensable. In addition, many non-coding regions, including regulatory elements and non-coding RNAs, are critical for mycobacterial growth. Our analysis shows that the genetic requirements for growth are more complex than can be appreciated using gene-centric analysis. |
| first_indexed | 2024-12-13T14:21:09Z |
| format | Article |
| id | doaj.art-6682f3185353438f90677a3b6043fb3f |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-13T14:21:09Z |
| publishDate | 2012-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-6682f3185353438f90677a3b6043fb3f2022-12-21T23:42:06ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742012-09-0189e100294610.1371/journal.ppat.1002946Global assessment of genomic regions required for growth in Mycobacterium tuberculosis.Yanjia J ZhangThomas R IoergerCurtis HuttenhowerJarukit E LongChristopher M SassettiJames C SacchettiniEric J RubinIdentifying genomic elements required for viability is central to our understanding of the basic physiology of bacterial pathogens. Recently, the combination of high-density mutagenesis and deep sequencing has allowed for the identification of required and conditionally required genes in many bacteria. Genes, however, make up only a part of the complex genomes of important bacterial pathogens. Here, we use an unbiased analysis to comprehensively identify genomic regions, including genes, domains, and intergenic elements, required for the optimal growth of Mycobacterium tuberculosis, a major global health pathogen. We found that several proteins jointly contain both domains required for optimal growth and domains that are dispensable. In addition, many non-coding regions, including regulatory elements and non-coding RNAs, are critical for mycobacterial growth. Our analysis shows that the genetic requirements for growth are more complex than can be appreciated using gene-centric analysis.http://europepmc.org/articles/PMC3460630?pdf=render |
| spellingShingle | Yanjia J Zhang Thomas R Ioerger Curtis Huttenhower Jarukit E Long Christopher M Sassetti James C Sacchettini Eric J Rubin Global assessment of genomic regions required for growth in Mycobacterium tuberculosis. PLoS Pathogens |
| title | Global assessment of genomic regions required for growth in Mycobacterium tuberculosis. |
| title_full | Global assessment of genomic regions required for growth in Mycobacterium tuberculosis. |
| title_fullStr | Global assessment of genomic regions required for growth in Mycobacterium tuberculosis. |
| title_full_unstemmed | Global assessment of genomic regions required for growth in Mycobacterium tuberculosis. |
| title_short | Global assessment of genomic regions required for growth in Mycobacterium tuberculosis. |
| title_sort | global assessment of genomic regions required for growth in mycobacterium tuberculosis |
| url | http://europepmc.org/articles/PMC3460630?pdf=render |
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