Identification and Effects of Skim Milk-Derived Bioactive Antihypertensive Peptides
Bioactive peptides are generated during milk fermentation or enzymatic hydrolysis. <i>Lactobacillus</i> (<i>L</i>) <i>helveticus</i> is commonly used to produce some types of fermented milk products. Fermented milk derived bioactive peptides are known to be benefi...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-12-01
|
| Series: | Biologics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-8449/2/1/1 |
| _version_ | 1811271484934455296 |
|---|---|
| author | Fatah B. Ahtesh Lily Stojanovska Vijay Mishra Osaana Donkor Jack Feehan Marijan Bosevski Michael Mathai Vasso Apostolopoulos |
| author_facet | Fatah B. Ahtesh Lily Stojanovska Vijay Mishra Osaana Donkor Jack Feehan Marijan Bosevski Michael Mathai Vasso Apostolopoulos |
| author_sort | Fatah B. Ahtesh |
| collection | DOAJ |
| description | Bioactive peptides are generated during milk fermentation or enzymatic hydrolysis. <i>Lactobacillus</i> (<i>L</i>) <i>helveticus</i> is commonly used to produce some types of fermented milk products. Fermented milk derived bioactive peptides are known to be beneficial in human health. Anti-hypertensive peptides play a dual role in the regulation of hypertension through the production of the vasoconstrictor angiotensin II and its inactivation of the vasodilator bradykinin. MALDI MS/MS, nano-LC/MS/MS and RP-HPLC were used to isolate peptides showing angiotensin converting enzyme inhibition (ACE-I) from 12% fermented skim milk using a combination of <i>L. helveticus</i> and Flavourzyme<sup>®</sup>. The fermentation procedure facilitated the identification of 133 anti-hypertensive peptides and 75% short chain amino acids, and the three with the highest ACE-I activity reduced blood pressure in a rat model of hypertension. The freeze- dried extract was supplemented in rodent chow. In this study 14-week-old male spontaneously hypertensive rats were fed for 10 weeks with the identified peptides added to chow and compared to controls supplemented with skim milk powder. Blood pressure (BP) decreased significantly (<i>p</i> < 0.05) from 6 to 10 weeks of FS groups (120/65 mmHg) compared with the NFS control groups, where the BP increased significantly (220/150 mmHg) (<i>p</i> < 0.05). The F6 fraction provided bioactive peptides with stronger antihypertensive properties than other fractions. Skim milk fermented by <i>L. helveticus</i> and Flavourzyme<sup>®</sup> generates several bioactive peptides which have a blood pressure lowering effect in hypertensive disease. |
| first_indexed | 2024-04-12T22:21:45Z |
| format | Article |
| id | doaj.art-66aaffa10f374fd1a510fc61c0fb5a81 |
| institution | Directory Open Access Journal |
| issn | 2673-8449 |
| language | English |
| last_indexed | 2024-04-12T22:21:45Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biologics |
| spelling | doaj.art-66aaffa10f374fd1a510fc61c0fb5a812022-12-22T03:14:21ZengMDPI AGBiologics2673-84492021-12-012111410.3390/biologics2010001Identification and Effects of Skim Milk-Derived Bioactive Antihypertensive PeptidesFatah B. Ahtesh0Lily Stojanovska1Vijay Mishra2Osaana Donkor3Jack Feehan4Marijan Bosevski5Michael Mathai6Vasso Apostolopoulos7Institute for Health and Sport, Victoria University, Melbourne 3011, AustraliaDepartment of Nutrition and Health, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain 999041, United Arab EmiratesInstitute for Sustainable Industries and Liveable Cities, Victoria University, Melbourne 3011, AustraliaInstitute for Sustainable Industries and Liveable Cities, Victoria University, Melbourne 3011, AustraliaInstitute for Health and Sport, Victoria University, Melbourne 3011, AustraliaSt. Cyril and Methodius, Faculty of Medicine, University Cardiology Clinic, 1000 Skopje, North MacedoniaInstitute for Health and Sport, Victoria University, Melbourne 3011, AustraliaInstitute for Health and Sport, Victoria University, Melbourne 3011, AustraliaBioactive peptides are generated during milk fermentation or enzymatic hydrolysis. <i>Lactobacillus</i> (<i>L</i>) <i>helveticus</i> is commonly used to produce some types of fermented milk products. Fermented milk derived bioactive peptides are known to be beneficial in human health. Anti-hypertensive peptides play a dual role in the regulation of hypertension through the production of the vasoconstrictor angiotensin II and its inactivation of the vasodilator bradykinin. MALDI MS/MS, nano-LC/MS/MS and RP-HPLC were used to isolate peptides showing angiotensin converting enzyme inhibition (ACE-I) from 12% fermented skim milk using a combination of <i>L. helveticus</i> and Flavourzyme<sup>®</sup>. The fermentation procedure facilitated the identification of 133 anti-hypertensive peptides and 75% short chain amino acids, and the three with the highest ACE-I activity reduced blood pressure in a rat model of hypertension. The freeze- dried extract was supplemented in rodent chow. In this study 14-week-old male spontaneously hypertensive rats were fed for 10 weeks with the identified peptides added to chow and compared to controls supplemented with skim milk powder. Blood pressure (BP) decreased significantly (<i>p</i> < 0.05) from 6 to 10 weeks of FS groups (120/65 mmHg) compared with the NFS control groups, where the BP increased significantly (220/150 mmHg) (<i>p</i> < 0.05). The F6 fraction provided bioactive peptides with stronger antihypertensive properties than other fractions. Skim milk fermented by <i>L. helveticus</i> and Flavourzyme<sup>®</sup> generates several bioactive peptides which have a blood pressure lowering effect in hypertensive disease.https://www.mdpi.com/2673-8449/2/1/1anti-hypertensive peptidesFlavourzyme<sup>®</sup><i>Lactobacillus helveticus</i>blood pressureratesfermented skim milk |
| spellingShingle | Fatah B. Ahtesh Lily Stojanovska Vijay Mishra Osaana Donkor Jack Feehan Marijan Bosevski Michael Mathai Vasso Apostolopoulos Identification and Effects of Skim Milk-Derived Bioactive Antihypertensive Peptides Biologics anti-hypertensive peptides Flavourzyme<sup>®</sup> <i>Lactobacillus helveticus</i> blood pressure rates fermented skim milk |
| title | Identification and Effects of Skim Milk-Derived Bioactive Antihypertensive Peptides |
| title_full | Identification and Effects of Skim Milk-Derived Bioactive Antihypertensive Peptides |
| title_fullStr | Identification and Effects of Skim Milk-Derived Bioactive Antihypertensive Peptides |
| title_full_unstemmed | Identification and Effects of Skim Milk-Derived Bioactive Antihypertensive Peptides |
| title_short | Identification and Effects of Skim Milk-Derived Bioactive Antihypertensive Peptides |
| title_sort | identification and effects of skim milk derived bioactive antihypertensive peptides |
| topic | anti-hypertensive peptides Flavourzyme<sup>®</sup> <i>Lactobacillus helveticus</i> blood pressure rates fermented skim milk |
| url | https://www.mdpi.com/2673-8449/2/1/1 |
| work_keys_str_mv | AT fatahbahtesh identificationandeffectsofskimmilkderivedbioactiveantihypertensivepeptides AT lilystojanovska identificationandeffectsofskimmilkderivedbioactiveantihypertensivepeptides AT vijaymishra identificationandeffectsofskimmilkderivedbioactiveantihypertensivepeptides AT osaanadonkor identificationandeffectsofskimmilkderivedbioactiveantihypertensivepeptides AT jackfeehan identificationandeffectsofskimmilkderivedbioactiveantihypertensivepeptides AT marijanbosevski identificationandeffectsofskimmilkderivedbioactiveantihypertensivepeptides AT michaelmathai identificationandeffectsofskimmilkderivedbioactiveantihypertensivepeptides AT vassoapostolopoulos identificationandeffectsofskimmilkderivedbioactiveantihypertensivepeptides |