FMLRC: Hybrid long read error correction using an FM-index
Abstract Background Long read sequencing is changing the landscape of genomic research, especially de novo assembly. Despite the high error rate inherent to long read technologies, increased read lengths dramatically improve the continuity and accuracy of genome assemblies. However, the cost and thr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-02-01
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| Series: | BMC Bioinformatics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12859-018-2051-3 |
| _version_ | 1811265313778434048 |
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| author | Jeremy R. Wang James Holt Leonard McMillan Corbin D. Jones |
| author_facet | Jeremy R. Wang James Holt Leonard McMillan Corbin D. Jones |
| author_sort | Jeremy R. Wang |
| collection | DOAJ |
| description | Abstract Background Long read sequencing is changing the landscape of genomic research, especially de novo assembly. Despite the high error rate inherent to long read technologies, increased read lengths dramatically improve the continuity and accuracy of genome assemblies. However, the cost and throughput of these technologies limits their application to complex genomes. One solution is to decrease the cost and time to assemble novel genomes by leveraging “hybrid” assemblies that use long reads for scaffolding and short reads for accuracy. Results We describe a novel method leveraging a multi-string Burrows-Wheeler Transform with auxiliary FM-index to correct errors in long read sequences using a set of complementary short reads. We demonstrate that our method efficiently produces significantly more high quality corrected sequence than existing hybrid error-correction methods. We also show that our method produces more contiguous assemblies, in many cases, than existing state-of-the-art hybrid and long-read only de novo assembly methods. Conclusion Our method accurately corrects long read sequence data using complementary short reads. We demonstrate higher total throughput of corrected long reads and a corresponding increase in contiguity of the resulting de novo assemblies. Improved throughput and computational efficiency than existing methods will help better economically utilize emerging long read sequencing technologies. |
| first_indexed | 2024-04-12T20:20:38Z |
| format | Article |
| id | doaj.art-66b022e366b14f87b35a8b95e1fc3194 |
| institution | Directory Open Access Journal |
| issn | 1471-2105 |
| language | English |
| last_indexed | 2024-04-12T20:20:38Z |
| publishDate | 2018-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Bioinformatics |
| spelling | doaj.art-66b022e366b14f87b35a8b95e1fc31942022-12-22T03:18:00ZengBMCBMC Bioinformatics1471-21052018-02-0119111110.1186/s12859-018-2051-3FMLRC: Hybrid long read error correction using an FM-indexJeremy R. Wang0James Holt1Leonard McMillan2Corbin D. Jones3Department of Genetics, University of North Carolina at Chapel HillDepartment of Computer Science, University of North Carolina at Chapel HillDepartment of Computer Science, University of North Carolina at Chapel HillDepartment of Biology and Integrative Program for Biological and Genome Sciences, University of North Carolina at Chapel HillAbstract Background Long read sequencing is changing the landscape of genomic research, especially de novo assembly. Despite the high error rate inherent to long read technologies, increased read lengths dramatically improve the continuity and accuracy of genome assemblies. However, the cost and throughput of these technologies limits their application to complex genomes. One solution is to decrease the cost and time to assemble novel genomes by leveraging “hybrid” assemblies that use long reads for scaffolding and short reads for accuracy. Results We describe a novel method leveraging a multi-string Burrows-Wheeler Transform with auxiliary FM-index to correct errors in long read sequences using a set of complementary short reads. We demonstrate that our method efficiently produces significantly more high quality corrected sequence than existing hybrid error-correction methods. We also show that our method produces more contiguous assemblies, in many cases, than existing state-of-the-art hybrid and long-read only de novo assembly methods. Conclusion Our method accurately corrects long read sequence data using complementary short reads. We demonstrate higher total throughput of corrected long reads and a corresponding increase in contiguity of the resulting de novo assemblies. Improved throughput and computational efficiency than existing methods will help better economically utilize emerging long read sequencing technologies.http://link.springer.com/article/10.1186/s12859-018-2051-3de novo assemblyHybrid error correctionLong readPacbioBWTFM-Index |
| spellingShingle | Jeremy R. Wang James Holt Leonard McMillan Corbin D. Jones FMLRC: Hybrid long read error correction using an FM-index BMC Bioinformatics de novo assembly Hybrid error correction Long read Pacbio BWT FM-Index |
| title | FMLRC: Hybrid long read error correction using an FM-index |
| title_full | FMLRC: Hybrid long read error correction using an FM-index |
| title_fullStr | FMLRC: Hybrid long read error correction using an FM-index |
| title_full_unstemmed | FMLRC: Hybrid long read error correction using an FM-index |
| title_short | FMLRC: Hybrid long read error correction using an FM-index |
| title_sort | fmlrc hybrid long read error correction using an fm index |
| topic | de novo assembly Hybrid error correction Long read Pacbio BWT FM-Index |
| url | http://link.springer.com/article/10.1186/s12859-018-2051-3 |
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