Epigenetic regulation of Elf5 is associated with epithelial-mesenchymal transition in urothelial cancer.
E74-like factor 5 (Elf5) has been associated with tumor suppression in breast cancer. However, its role in urothelial cancer (UC) is completely unknown. Immunohistochemistry (IHC) and methylation specific PCR (MSP) were done to detect Elf5 expression level and its promoter methylation. Results revea...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4309403?pdf=render |
| _version_ | 1818193253533483008 |
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| author | Bo Wu Xiaoming Cao Xuezhi Liang Xuhui Zhang Wei Zhang Guang Sun Dongwen Wang |
| author_facet | Bo Wu Xiaoming Cao Xuezhi Liang Xuhui Zhang Wei Zhang Guang Sun Dongwen Wang |
| author_sort | Bo Wu |
| collection | DOAJ |
| description | E74-like factor 5 (Elf5) has been associated with tumor suppression in breast cancer. However, its role in urothelial cancer (UC) is completely unknown. Immunohistochemistry (IHC) and methylation specific PCR (MSP) were done to detect Elf5 expression level and its promoter methylation. Results revealed that low expression of Elf5 on protein and mRNA levels were associated with tumor progression, early relapse and poor survival. In vitro, down-regulation of Elf5 can increase epithelial-mesenchymal transition (EMT). Aberrant Elf5 methylation was identified as major mechanism for Elf5 gene silence. Accordingly, restoration of Elf5 by infection or demethylating treatment effectively reversed EMT processes. In conclusion, we identified Elf5 as a novel biomarker of UC on several biological levels and established a causative link between Elf5 and EMT in UC. |
| first_indexed | 2024-12-12T00:43:28Z |
| format | Article |
| id | doaj.art-66bf2702a7ce4c6c94a91a1848a46fa4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T00:43:28Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-66bf2702a7ce4c6c94a91a1848a46fa42022-12-22T00:44:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01101e011751010.1371/journal.pone.0117510Epigenetic regulation of Elf5 is associated with epithelial-mesenchymal transition in urothelial cancer.Bo WuXiaoming CaoXuezhi LiangXuhui ZhangWei ZhangGuang SunDongwen WangE74-like factor 5 (Elf5) has been associated with tumor suppression in breast cancer. However, its role in urothelial cancer (UC) is completely unknown. Immunohistochemistry (IHC) and methylation specific PCR (MSP) were done to detect Elf5 expression level and its promoter methylation. Results revealed that low expression of Elf5 on protein and mRNA levels were associated with tumor progression, early relapse and poor survival. In vitro, down-regulation of Elf5 can increase epithelial-mesenchymal transition (EMT). Aberrant Elf5 methylation was identified as major mechanism for Elf5 gene silence. Accordingly, restoration of Elf5 by infection or demethylating treatment effectively reversed EMT processes. In conclusion, we identified Elf5 as a novel biomarker of UC on several biological levels and established a causative link between Elf5 and EMT in UC.http://europepmc.org/articles/PMC4309403?pdf=render |
| spellingShingle | Bo Wu Xiaoming Cao Xuezhi Liang Xuhui Zhang Wei Zhang Guang Sun Dongwen Wang Epigenetic regulation of Elf5 is associated with epithelial-mesenchymal transition in urothelial cancer. PLoS ONE |
| title | Epigenetic regulation of Elf5 is associated with epithelial-mesenchymal transition in urothelial cancer. |
| title_full | Epigenetic regulation of Elf5 is associated with epithelial-mesenchymal transition in urothelial cancer. |
| title_fullStr | Epigenetic regulation of Elf5 is associated with epithelial-mesenchymal transition in urothelial cancer. |
| title_full_unstemmed | Epigenetic regulation of Elf5 is associated with epithelial-mesenchymal transition in urothelial cancer. |
| title_short | Epigenetic regulation of Elf5 is associated with epithelial-mesenchymal transition in urothelial cancer. |
| title_sort | epigenetic regulation of elf5 is associated with epithelial mesenchymal transition in urothelial cancer |
| url | http://europepmc.org/articles/PMC4309403?pdf=render |
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