Performance of Caffeine Content Analysis in Robusta sp and Theobroma cacao L using Iodometry, UV-Vis Spectrophotometry and High Performance Liquid Chromatography
Caffeine is a xanthine alkaloid compound found in Robusta sp and Theobroma cacao L. The caffeine extract was obtained by evaporating CHCl3 using a rotary evaporator and determine its content using iodometry, UV-Vis spectrophotometry and High Performance Liquid Chromatography, then validated by measu...
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| Format: | Article |
| Language: | English |
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University of Brawijaya
2020-12-01
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| Series: | Journal of Pure and Applied Chemistry Research |
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| Online Access: | https://jpacr.ub.ac.id/index.php/jpacr/article/view/535/pdf |
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| author | Yeanchon H. Dulanlebit Nikmans Hattu Ritti P. Sangadji Risma Mahulette Nataniel Kelmanutu Sandriliati Asari |
| author_facet | Yeanchon H. Dulanlebit Nikmans Hattu Ritti P. Sangadji Risma Mahulette Nataniel Kelmanutu Sandriliati Asari |
| author_sort | Yeanchon H. Dulanlebit |
| collection | DOAJ |
| description | Caffeine is a xanthine alkaloid compound found in Robusta sp and Theobroma cacao L. The caffeine extract was obtained by evaporating CHCl3 using a rotary evaporator and determine its content using iodometry, UV-Vis spectrophotometry and High Performance Liquid Chromatography, then validated by measurement methods. The iodometric caffeine content in Theobroma cacao L is 900 mg/kg while UV-Vis spectrophotometry is 4,000 mg/kg, and for HPLC in Robusta sp is 19,475 mg/kg. The iodometry performance gives the linearity value, R2 of 0.9791. The precision values at 5.0 mg/L and 15.0 mg/L are 4.6% and 8.3%. Accuracy for 5.0; 10.0; and 15.0 mg/L are 106%, 100% and 94% respectively. The performance of UV-Vis spectrophotometry gives linearity value, R2 of 0.9948. The precision value at 1.0 mg/L and 6.0 mg/L gives the variance coefficient value of 4.2% and 1.2%, while the accuracy at 1.0 mg/L and 6.0 mg/L are 99% and 101%. |
| first_indexed | 2024-03-11T18:41:42Z |
| format | Article |
| id | doaj.art-66dd9737b0d3408e883b5b1adc61a839 |
| institution | Directory Open Access Journal |
| issn | 2302-4690 2541-0733 |
| language | English |
| last_indexed | 2024-03-11T18:41:42Z |
| publishDate | 2020-12-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Pure and Applied Chemistry Research |
| spelling | doaj.art-66dd9737b0d3408e883b5b1adc61a8392023-10-12T12:52:13ZengUniversity of BrawijayaJournal of Pure and Applied Chemistry Research2302-46902541-07332020-12-01093212221Performance of Caffeine Content Analysis in Robusta sp and Theobroma cacao L using Iodometry, UV-Vis Spectrophotometry and High Performance Liquid ChromatographyYeanchon H. Dulanlebit0 Nikmans Hattu1Ritti P. Sangadji2Risma Mahulette3 Nataniel Kelmanutu4Sandriliati Asari5Chemistry Study Program, Faculty of Education and Teacher Training - Pattimura UniversityChemistry Department, Faculty of Mathematics and Natural Science - Pattimura UniversityFood and Drug Authority Office in Tanimbar Island RegionChemistry Study Program, Faculty of Education and Teacher Training - Pattimura UniversityChemistry Study Program, Faculty of Education and Teacher Training - Pattimura UniversityChemistry Study Program, Faculty of Education and Teacher Training - Pattimura UniversityCaffeine is a xanthine alkaloid compound found in Robusta sp and Theobroma cacao L. The caffeine extract was obtained by evaporating CHCl3 using a rotary evaporator and determine its content using iodometry, UV-Vis spectrophotometry and High Performance Liquid Chromatography, then validated by measurement methods. The iodometric caffeine content in Theobroma cacao L is 900 mg/kg while UV-Vis spectrophotometry is 4,000 mg/kg, and for HPLC in Robusta sp is 19,475 mg/kg. The iodometry performance gives the linearity value, R2 of 0.9791. The precision values at 5.0 mg/L and 15.0 mg/L are 4.6% and 8.3%. Accuracy for 5.0; 10.0; and 15.0 mg/L are 106%, 100% and 94% respectively. The performance of UV-Vis spectrophotometry gives linearity value, R2 of 0.9948. The precision value at 1.0 mg/L and 6.0 mg/L gives the variance coefficient value of 4.2% and 1.2%, while the accuracy at 1.0 mg/L and 6.0 mg/L are 99% and 101%.https://jpacr.ub.ac.id/index.php/jpacr/article/view/535/pdfcaffeinehplciodometryrobusta spspectrophotometrytheobroma cacao l |
| spellingShingle | Yeanchon H. Dulanlebit Nikmans Hattu Ritti P. Sangadji Risma Mahulette Nataniel Kelmanutu Sandriliati Asari Performance of Caffeine Content Analysis in Robusta sp and Theobroma cacao L using Iodometry, UV-Vis Spectrophotometry and High Performance Liquid Chromatography Journal of Pure and Applied Chemistry Research caffeine hplc iodometry robusta sp spectrophotometry theobroma cacao l |
| title | Performance of Caffeine Content Analysis in Robusta sp and Theobroma cacao L using Iodometry, UV-Vis Spectrophotometry and High Performance Liquid Chromatography |
| title_full | Performance of Caffeine Content Analysis in Robusta sp and Theobroma cacao L using Iodometry, UV-Vis Spectrophotometry and High Performance Liquid Chromatography |
| title_fullStr | Performance of Caffeine Content Analysis in Robusta sp and Theobroma cacao L using Iodometry, UV-Vis Spectrophotometry and High Performance Liquid Chromatography |
| title_full_unstemmed | Performance of Caffeine Content Analysis in Robusta sp and Theobroma cacao L using Iodometry, UV-Vis Spectrophotometry and High Performance Liquid Chromatography |
| title_short | Performance of Caffeine Content Analysis in Robusta sp and Theobroma cacao L using Iodometry, UV-Vis Spectrophotometry and High Performance Liquid Chromatography |
| title_sort | performance of caffeine content analysis in robusta sp and theobroma cacao l using iodometry uv vis spectrophotometry and high performance liquid chromatography |
| topic | caffeine hplc iodometry robusta sp spectrophotometry theobroma cacao l |
| url | https://jpacr.ub.ac.id/index.php/jpacr/article/view/535/pdf |
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