Application of High Resolution Melt analysis (HRM) for screening haplotype variation in a non-model plant genus: Cyclopia (Honeybush)

Aim This study has three broad aims: to (a) develop genus-specific primers for High Resolution Melt analysis (HRM) of members of Cyclopia Vent., (b) test the haplotype discrimination of HRM compared to Sanger sequencing, and (c) provide an example of using HRM to detect novel haplotype variation in...

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Bibliographic Details
Main Authors: Nicholas C. Galuszynski, Alastair J. Potts
Format: Article
Language:English
Published: PeerJ Inc. 2020-05-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/9187.pdf