Application of High Resolution Melt analysis (HRM) for screening haplotype variation in a non-model plant genus: Cyclopia (Honeybush)
Aim This study has three broad aims: to (a) develop genus-specific primers for High Resolution Melt analysis (HRM) of members of Cyclopia Vent., (b) test the haplotype discrimination of HRM compared to Sanger sequencing, and (c) provide an example of using HRM to detect novel haplotype variation in...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
PeerJ Inc.
2020-05-01
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Series: | PeerJ |
Subjects: | |
Online Access: | https://peerj.com/articles/9187.pdf |