Dendritic cell ICAM-1 strengthens synapses with CD8 T cells but is not required for their early differentiation
Summary: Lymphocyte priming in lymph nodes (LNs) was postulated to depend on the formation of stable T cell receptor (TCR)-specific immune synapses (ISs) with antigen (Ag)-presenting dendritic cells (DCs). The high-affinity LFA-1 ligand ICAM-1 was implicated in different ISs studied in vitro. We dis...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723008756 |
| _version_ | 1797731089269129216 |
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| author | Anita Sapoznikov Stav Kozlovski Nehora Levi Sara W. Feigelson Ofer Regev Natalia Davidzohn Shifra Ben-Dor Rebecca Haffner-Krausz Ester Feldmesser Noa Wigoda Ekaterina Petrovich-Kopitman Moshe Biton Ronen Alon |
| author_facet | Anita Sapoznikov Stav Kozlovski Nehora Levi Sara W. Feigelson Ofer Regev Natalia Davidzohn Shifra Ben-Dor Rebecca Haffner-Krausz Ester Feldmesser Noa Wigoda Ekaterina Petrovich-Kopitman Moshe Biton Ronen Alon |
| author_sort | Anita Sapoznikov |
| collection | DOAJ |
| description | Summary: Lymphocyte priming in lymph nodes (LNs) was postulated to depend on the formation of stable T cell receptor (TCR)-specific immune synapses (ISs) with antigen (Ag)-presenting dendritic cells (DCs). The high-affinity LFA-1 ligand ICAM-1 was implicated in different ISs studied in vitro. We dissect the in vivo roles of endogenous DC ICAM-1 in Ag-stimulated T cell proliferation and differentiation and find that under type 1 polarizing conditions in vaccinated or vaccinia virus-infected skin-draining LNs, Ag-presenting DCs engage in ICAM-1-dependent stable conjugates with a subset of Ag-specific CD8 blasts. Nevertheless, in the absence of these conjugates, CD8 lymphocyte proliferation and differentiation into functional cytotoxic T cells (CTLs) and skin homing effector lymphocytes takes place normally. Our results suggest that although CD8 T cell blasts engage in tight ICAM-1-dependent DC-T ISs, firm ISs are dispensable for TCR-triggered proliferation and differentiation into productive effector lymphocytes. |
| first_indexed | 2024-03-12T11:53:43Z |
| format | Article |
| id | doaj.art-67139ba272364b81bc07233d20deeb03 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-12T11:53:43Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-67139ba272364b81bc07233d20deeb032023-08-31T05:01:56ZengElsevierCell Reports2211-12472023-08-01428112864Dendritic cell ICAM-1 strengthens synapses with CD8 T cells but is not required for their early differentiationAnita Sapoznikov0Stav Kozlovski1Nehora Levi2Sara W. Feigelson3Ofer Regev4Natalia Davidzohn5Shifra Ben-Dor6Rebecca Haffner-Krausz7Ester Feldmesser8Noa Wigoda9Ekaterina Petrovich-Kopitman10Moshe Biton11Ronen Alon12Deptartment of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, IsraelDeptartment of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, IsraelDeptartment of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, IsraelDeptartment of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, IsraelDeptartment of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, IsraelDeptartment of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, IsraelDepartment of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, IsraelDepartment of Veterinary Resources, Weizmann Institute of Science, Rehovot, IsraelDepartment of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, IsraelDepartment of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, IsraelDepartment of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, IsraelDeptartment of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, Israel; Corresponding authorDeptartment of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, Israel; Corresponding authorSummary: Lymphocyte priming in lymph nodes (LNs) was postulated to depend on the formation of stable T cell receptor (TCR)-specific immune synapses (ISs) with antigen (Ag)-presenting dendritic cells (DCs). The high-affinity LFA-1 ligand ICAM-1 was implicated in different ISs studied in vitro. We dissect the in vivo roles of endogenous DC ICAM-1 in Ag-stimulated T cell proliferation and differentiation and find that under type 1 polarizing conditions in vaccinated or vaccinia virus-infected skin-draining LNs, Ag-presenting DCs engage in ICAM-1-dependent stable conjugates with a subset of Ag-specific CD8 blasts. Nevertheless, in the absence of these conjugates, CD8 lymphocyte proliferation and differentiation into functional cytotoxic T cells (CTLs) and skin homing effector lymphocytes takes place normally. Our results suggest that although CD8 T cell blasts engage in tight ICAM-1-dependent DC-T ISs, firm ISs are dispensable for TCR-triggered proliferation and differentiation into productive effector lymphocytes.http://www.sciencedirect.com/science/article/pii/S2211124723008756CP: Immunology |
| spellingShingle | Anita Sapoznikov Stav Kozlovski Nehora Levi Sara W. Feigelson Ofer Regev Natalia Davidzohn Shifra Ben-Dor Rebecca Haffner-Krausz Ester Feldmesser Noa Wigoda Ekaterina Petrovich-Kopitman Moshe Biton Ronen Alon Dendritic cell ICAM-1 strengthens synapses with CD8 T cells but is not required for their early differentiation Cell Reports CP: Immunology |
| title | Dendritic cell ICAM-1 strengthens synapses with CD8 T cells but is not required for their early differentiation |
| title_full | Dendritic cell ICAM-1 strengthens synapses with CD8 T cells but is not required for their early differentiation |
| title_fullStr | Dendritic cell ICAM-1 strengthens synapses with CD8 T cells but is not required for their early differentiation |
| title_full_unstemmed | Dendritic cell ICAM-1 strengthens synapses with CD8 T cells but is not required for their early differentiation |
| title_short | Dendritic cell ICAM-1 strengthens synapses with CD8 T cells but is not required for their early differentiation |
| title_sort | dendritic cell icam 1 strengthens synapses with cd8 t cells but is not required for their early differentiation |
| topic | CP: Immunology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723008756 |
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