Development of a universal endogenous qPCR control for eukaryotic DNA samples

Development of a universal endogenous qPCR control for eukaryotic DNA samples

Abstract Background Phytoplasma are obligate intracellular plant-pathogenic bacteria that infect a broad range of plant species and are transmitted by different insect species. Quantitative real-time PCR (qPCR) is one of the most commonly used techniques for pathogen detection, especially for pathog...

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Bibliographic Details
Main Authors: Cecilia Mittelberger, Lisa Obkircher, Vicky Oberkofler, Alan Ianeselli, Christine Kerschbamer, Andreas Gallmetzer, Yazmid Reyes-Dominguez, Thomas Letschka, Katrin Janik
Format: Article
Language:English
Published: BMC 2020-04-01
Series:Plant Methods
Subjects:
Quantitative real-time PCR
Pathogen detection
Endogenous control
Reference gene
Phytoplasma
Online Access:http://link.springer.com/article/10.1186/s13007-020-00597-2

Internet

http://link.springer.com/article/10.1186/s13007-020-00597-2

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