Establishment of a Direct PCR Assay for Simultaneous Differential Diagnosis of African Swine Fever and Classical Swine Fever Using Crude Tissue Samples

Establishment of a Direct PCR Assay for Simultaneous Differential Diagnosis of African Swine Fever and Classical Swine Fever Using Crude Tissue Samples

African swine fever (ASF) and classical swine fever (CSF) are contagious swine diseases that are clinically indistinguishable from each other; hence, reliable test methods for accurate diagnosis and differentiation are highly demanded. By employing a buffer system suitable for crude extraction of nu...

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Bibliographic Details
Main Authors: Tatsuya Nishi, Kota Okadera, Katsuhiko Fukai, Miwa Yoshizaki, Ai Nakasuji, Syuji Yoneyama, Takehiro Kokuho
Format: Article
Language:English
Published: MDPI AG 2022-02-01
Series:Viruses
Subjects:
African swine fever
classical swine fever
real-time reverse-transcription polymerase chain reaction
multiplex detection
crude nucleic acid extraction
Online Access:https://www.mdpi.com/1999-4915/14/3/498

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https://www.mdpi.com/1999-4915/14/3/498

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