Rnd3 induces stress fibres in endothelial cells through RhoB
Summary Rnd proteins are atypical Rho family proteins that do not hydrolyse GTP and are instead regulated by expression levels and post-translational modifications. Rnd1 and Rnd3/RhoE induce loss of actin stress fibres and cell rounding in multiple cell types, whereas responses to Rnd2 are more vari...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The Company of Biologists
2012-12-01
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| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/2/2/210 |
| _version_ | 1818656805650169856 |
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| author | Undine Gottesbühren Ritu Garg Philippe Riou Brad McColl Daniel Brayson Anne J. Ridley |
| author_facet | Undine Gottesbühren Ritu Garg Philippe Riou Brad McColl Daniel Brayson Anne J. Ridley |
| author_sort | Undine Gottesbühren |
| collection | DOAJ |
| description | Summary
Rnd proteins are atypical Rho family proteins that do not hydrolyse GTP and are instead regulated by expression levels and post-translational modifications. Rnd1 and Rnd3/RhoE induce loss of actin stress fibres and cell rounding in multiple cell types, whereas responses to Rnd2 are more variable. Here we report the responses of endothelial cells to Rnd proteins. Rnd3 induces a very transient decrease in stress fibres but subsequently stimulates a strong increase in stress fibres, in contrast to the reduction observed in other cell types. Rnd2 also increases stress fibres whereas Rnd1 induces a loss of stress fibres and weakening of cell–cell junctions. Rnd3 does not act through any of its known signalling partners and does not need to associate with membranes to increase stress fibres. Instead, it acts by increasing RhoB expression, which is then required for Rnd3-induced stress fibre assembly. Rnd2 also increases RhoB levels. These data indicate that the cytoskeletal response to Rnd3 expression is dependent on cell type and context, and identify regulation of RhoB as a new mechanism for Rnd proteins to affect the actin cytoskeleton. |
| first_indexed | 2024-12-17T03:31:25Z |
| format | Article |
| id | doaj.art-6767cc1517654a4ab14d52492dc2480d |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-17T03:31:25Z |
| publishDate | 2012-12-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-6767cc1517654a4ab14d52492dc2480d2022-12-21T22:05:16ZengThe Company of BiologistsBiology Open2046-63902012-12-012221021610.1242/bio.2012357420123574Rnd3 induces stress fibres in endothelial cells through RhoBUndine GottesbührenRitu GargPhilippe RiouBrad McCollDaniel BraysonAnne J. RidleySummary Rnd proteins are atypical Rho family proteins that do not hydrolyse GTP and are instead regulated by expression levels and post-translational modifications. Rnd1 and Rnd3/RhoE induce loss of actin stress fibres and cell rounding in multiple cell types, whereas responses to Rnd2 are more variable. Here we report the responses of endothelial cells to Rnd proteins. Rnd3 induces a very transient decrease in stress fibres but subsequently stimulates a strong increase in stress fibres, in contrast to the reduction observed in other cell types. Rnd2 also increases stress fibres whereas Rnd1 induces a loss of stress fibres and weakening of cell–cell junctions. Rnd3 does not act through any of its known signalling partners and does not need to associate with membranes to increase stress fibres. Instead, it acts by increasing RhoB expression, which is then required for Rnd3-induced stress fibre assembly. Rnd2 also increases RhoB levels. These data indicate that the cytoskeletal response to Rnd3 expression is dependent on cell type and context, and identify regulation of RhoB as a new mechanism for Rnd proteins to affect the actin cytoskeleton.http://bio.biologists.org/content/2/2/210Rho GTPasesEndothelial cellsActin filamentsRnd proteins |
| spellingShingle | Undine Gottesbühren Ritu Garg Philippe Riou Brad McColl Daniel Brayson Anne J. Ridley Rnd3 induces stress fibres in endothelial cells through RhoB Biology Open Rho GTPases Endothelial cells Actin filaments Rnd proteins |
| title | Rnd3 induces stress fibres in endothelial cells through RhoB |
| title_full | Rnd3 induces stress fibres in endothelial cells through RhoB |
| title_fullStr | Rnd3 induces stress fibres in endothelial cells through RhoB |
| title_full_unstemmed | Rnd3 induces stress fibres in endothelial cells through RhoB |
| title_short | Rnd3 induces stress fibres in endothelial cells through RhoB |
| title_sort | rnd3 induces stress fibres in endothelial cells through rhob |
| topic | Rho GTPases Endothelial cells Actin filaments Rnd proteins |
| url | http://bio.biologists.org/content/2/2/210 |
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