Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats
Background: Magnesium is a mineral that modulates several physiological processes. However, its relationship with intestinal microbiota has been scarcely studied. Therefore, this study aimed to assess the role of dietary magnesium content to modulate the intestinal microbiota of Wistar male rats. Me...
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MDPI AG
2020-09-01
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Online Access: | https://www.mdpi.com/2072-6643/12/9/2889 |
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author | Arantxa García-Legorreta Luis Alfonso Soriano-Pérez Aline Mariana Flores-Buendía Omar Noel Medina-Campos Lilia G. Noriega Omar Granados-Portillo Rafael Nambo-Venegas Armando R. Tovar Alfredo Mendoza-Vargas Diana Barrera-Oviedo José Pedraza-Chaverri Berenice Palacios-González |
author_facet | Arantxa García-Legorreta Luis Alfonso Soriano-Pérez Aline Mariana Flores-Buendía Omar Noel Medina-Campos Lilia G. Noriega Omar Granados-Portillo Rafael Nambo-Venegas Armando R. Tovar Alfredo Mendoza-Vargas Diana Barrera-Oviedo José Pedraza-Chaverri Berenice Palacios-González |
author_sort | Arantxa García-Legorreta |
collection | DOAJ |
description | Background: Magnesium is a mineral that modulates several physiological processes. However, its relationship with intestinal microbiota has been scarcely studied. Therefore, this study aimed to assess the role of dietary magnesium content to modulate the intestinal microbiota of Wistar male rats. Methods: Rats were randomly assigned one of three diets: a control diet (C-Mg; 1000 mg/kg), a low magnesium content diet (L-Mg; 60 mg/kg), and a high magnesium content diet (H-Mg; 6000 mg/kg), for two weeks. After treatment, fecal samples were collected. Microbiota composition was assessed by sequencing the V3–V4 hypervariable region. Results: The C-Mg and L-Mg groups had more diversity than H-Mg group. CF231, SMB53, <i>Dorea</i>, <i>Lactobacillus</i> and <i>Turibacter</i> were enriched in the L-Mg group. In contrast, the phyla <i>Proteobacteria</i>, <i>Parabacteroides</i>, <i>Butyricimonas</i>, and <i>Victivallis</i> were overrepresented in the H-Mg group. PICRUSt analysis indicated that fecal microbiota of the L-Mg group were encoded with an increased abundance of metabolic pathways involving carbohydrate metabolism and butanoate metabolism. Conclusion: Dietary magnesium supplementation can result in intestinal dysbiosis development in a situation where there is no magnesium deficiency. Conversely, low dietary magnesium consumption is associated with microbiota with a higher capacity to harvest energy from the diet. |
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issn | 2072-6643 |
language | English |
last_indexed | 2024-03-10T16:09:42Z |
publishDate | 2020-09-01 |
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spelling | doaj.art-67686bdd783f44299d69c2f1432930632023-11-20T14:35:42ZengMDPI AGNutrients2072-66432020-09-01129288910.3390/nu12092889Effect of Dietary Magnesium Content on Intestinal Microbiota of RatsArantxa García-Legorreta0Luis Alfonso Soriano-Pérez1Aline Mariana Flores-Buendía2Omar Noel Medina-Campos3Lilia G. Noriega4Omar Granados-Portillo5Rafael Nambo-Venegas6Armando R. Tovar7Alfredo Mendoza-Vargas8Diana Barrera-Oviedo9José Pedraza-Chaverri10Berenice Palacios-González11Unidad de Vinculación Científica de la Facultad de Medicina, de la Universidad Nacional Autónoma de Mexico en el Instituto Nacional de Medicina Genómica, Mexico City 14600, MexicoFacultad de Química, Departamento de Biología, Laboratorio F-315, Universidad Nacional Autónoma de Mexico, Mexico City 04510, MexicoFacultad de Química, Departamento de Biología, Laboratorio F-315, Universidad Nacional Autónoma de Mexico, Mexico City 04510, MexicoFacultad de Química, Departamento de Biología, Laboratorio F-315, Universidad Nacional Autónoma de Mexico, Mexico City 04510, MexicoDepartamento de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, Mexico City 14080, MexicoDepartamento de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, Mexico City 14080, MexicoLaboratorio de Bioquímica de Enfermedades Crónicas, Instituto Nacional de Medicina Genómica, Mexico City 14600, MexicoDepartamento de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, Mexico City 14080, MexicoUnidad de Secuenciación, Instituto Nacional de Medicina Genómica, Mexico City 14600, MexicoDepartamento de Farmacología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoFacultad de Química, Departamento de Biología, Laboratorio F-315, Universidad Nacional Autónoma de Mexico, Mexico City 04510, MexicoUnidad de Vinculación Científica de la Facultad de Medicina, de la Universidad Nacional Autónoma de Mexico en el Instituto Nacional de Medicina Genómica, Mexico City 14600, MexicoBackground: Magnesium is a mineral that modulates several physiological processes. However, its relationship with intestinal microbiota has been scarcely studied. Therefore, this study aimed to assess the role of dietary magnesium content to modulate the intestinal microbiota of Wistar male rats. Methods: Rats were randomly assigned one of three diets: a control diet (C-Mg; 1000 mg/kg), a low magnesium content diet (L-Mg; 60 mg/kg), and a high magnesium content diet (H-Mg; 6000 mg/kg), for two weeks. After treatment, fecal samples were collected. Microbiota composition was assessed by sequencing the V3–V4 hypervariable region. Results: The C-Mg and L-Mg groups had more diversity than H-Mg group. CF231, SMB53, <i>Dorea</i>, <i>Lactobacillus</i> and <i>Turibacter</i> were enriched in the L-Mg group. In contrast, the phyla <i>Proteobacteria</i>, <i>Parabacteroides</i>, <i>Butyricimonas</i>, and <i>Victivallis</i> were overrepresented in the H-Mg group. PICRUSt analysis indicated that fecal microbiota of the L-Mg group were encoded with an increased abundance of metabolic pathways involving carbohydrate metabolism and butanoate metabolism. Conclusion: Dietary magnesium supplementation can result in intestinal dysbiosis development in a situation where there is no magnesium deficiency. Conversely, low dietary magnesium consumption is associated with microbiota with a higher capacity to harvest energy from the diet.https://www.mdpi.com/2072-6643/12/9/2889dietary magnesiummineralsgut microbiotaWistar male rat |
spellingShingle | Arantxa García-Legorreta Luis Alfonso Soriano-Pérez Aline Mariana Flores-Buendía Omar Noel Medina-Campos Lilia G. Noriega Omar Granados-Portillo Rafael Nambo-Venegas Armando R. Tovar Alfredo Mendoza-Vargas Diana Barrera-Oviedo José Pedraza-Chaverri Berenice Palacios-González Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats Nutrients dietary magnesium minerals gut microbiota Wistar male rat |
title | Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats |
title_full | Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats |
title_fullStr | Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats |
title_full_unstemmed | Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats |
title_short | Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats |
title_sort | effect of dietary magnesium content on intestinal microbiota of rats |
topic | dietary magnesium minerals gut microbiota Wistar male rat |
url | https://www.mdpi.com/2072-6643/12/9/2889 |
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