Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats

Background: Magnesium is a mineral that modulates several physiological processes. However, its relationship with intestinal microbiota has been scarcely studied. Therefore, this study aimed to assess the role of dietary magnesium content to modulate the intestinal microbiota of Wistar male rats. Me...

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Main Authors: Arantxa García-Legorreta, Luis Alfonso Soriano-Pérez, Aline Mariana Flores-Buendía, Omar Noel Medina-Campos, Lilia G. Noriega, Omar Granados-Portillo, Rafael Nambo-Venegas, Armando R. Tovar, Alfredo Mendoza-Vargas, Diana Barrera-Oviedo, José Pedraza-Chaverri, Berenice Palacios-González
Format: Article
Language:English
Published: MDPI AG 2020-09-01
Series:Nutrients
Subjects:
Online Access:https://www.mdpi.com/2072-6643/12/9/2889
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author Arantxa García-Legorreta
Luis Alfonso Soriano-Pérez
Aline Mariana Flores-Buendía
Omar Noel Medina-Campos
Lilia G. Noriega
Omar Granados-Portillo
Rafael Nambo-Venegas
Armando R. Tovar
Alfredo Mendoza-Vargas
Diana Barrera-Oviedo
José Pedraza-Chaverri
Berenice Palacios-González
author_facet Arantxa García-Legorreta
Luis Alfonso Soriano-Pérez
Aline Mariana Flores-Buendía
Omar Noel Medina-Campos
Lilia G. Noriega
Omar Granados-Portillo
Rafael Nambo-Venegas
Armando R. Tovar
Alfredo Mendoza-Vargas
Diana Barrera-Oviedo
José Pedraza-Chaverri
Berenice Palacios-González
author_sort Arantxa García-Legorreta
collection DOAJ
description Background: Magnesium is a mineral that modulates several physiological processes. However, its relationship with intestinal microbiota has been scarcely studied. Therefore, this study aimed to assess the role of dietary magnesium content to modulate the intestinal microbiota of Wistar male rats. Methods: Rats were randomly assigned one of three diets: a control diet (C-Mg; 1000 mg/kg), a low magnesium content diet (L-Mg; 60 mg/kg), and a high magnesium content diet (H-Mg; 6000 mg/kg), for two weeks. After treatment, fecal samples were collected. Microbiota composition was assessed by sequencing the V3–V4 hypervariable region. Results: The C-Mg and L-Mg groups had more diversity than H-Mg group. CF231, SMB53, <i>Dorea</i>, <i>Lactobacillus</i> and <i>Turibacter</i> were enriched in the L-Mg group. In contrast, the phyla <i>Proteobacteria</i>, <i>Parabacteroides</i>, <i>Butyricimonas</i>, and <i>Victivallis</i> were overrepresented in the H-Mg group. PICRUSt analysis indicated that fecal microbiota of the L-Mg group were encoded with an increased abundance of metabolic pathways involving carbohydrate metabolism and butanoate metabolism. Conclusion: Dietary magnesium supplementation can result in intestinal dysbiosis development in a situation where there is no magnesium deficiency. Conversely, low dietary magnesium consumption is associated with microbiota with a higher capacity to harvest energy from the diet.
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spelling doaj.art-67686bdd783f44299d69c2f1432930632023-11-20T14:35:42ZengMDPI AGNutrients2072-66432020-09-01129288910.3390/nu12092889Effect of Dietary Magnesium Content on Intestinal Microbiota of RatsArantxa García-Legorreta0Luis Alfonso Soriano-Pérez1Aline Mariana Flores-Buendía2Omar Noel Medina-Campos3Lilia G. Noriega4Omar Granados-Portillo5Rafael Nambo-Venegas6Armando R. Tovar7Alfredo Mendoza-Vargas8Diana Barrera-Oviedo9José Pedraza-Chaverri10Berenice Palacios-González11Unidad de Vinculación Científica de la Facultad de Medicina, de la Universidad Nacional Autónoma de Mexico en el Instituto Nacional de Medicina Genómica, Mexico City 14600, MexicoFacultad de Química, Departamento de Biología, Laboratorio F-315, Universidad Nacional Autónoma de Mexico, Mexico City 04510, MexicoFacultad de Química, Departamento de Biología, Laboratorio F-315, Universidad Nacional Autónoma de Mexico, Mexico City 04510, MexicoFacultad de Química, Departamento de Biología, Laboratorio F-315, Universidad Nacional Autónoma de Mexico, Mexico City 04510, MexicoDepartamento de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, Mexico City 14080, MexicoDepartamento de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, Mexico City 14080, MexicoLaboratorio de Bioquímica de Enfermedades Crónicas, Instituto Nacional de Medicina Genómica, Mexico City 14600, MexicoDepartamento de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, Mexico City 14080, MexicoUnidad de Secuenciación, Instituto Nacional de Medicina Genómica, Mexico City 14600, MexicoDepartamento de Farmacología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoFacultad de Química, Departamento de Biología, Laboratorio F-315, Universidad Nacional Autónoma de Mexico, Mexico City 04510, MexicoUnidad de Vinculación Científica de la Facultad de Medicina, de la Universidad Nacional Autónoma de Mexico en el Instituto Nacional de Medicina Genómica, Mexico City 14600, MexicoBackground: Magnesium is a mineral that modulates several physiological processes. However, its relationship with intestinal microbiota has been scarcely studied. Therefore, this study aimed to assess the role of dietary magnesium content to modulate the intestinal microbiota of Wistar male rats. Methods: Rats were randomly assigned one of three diets: a control diet (C-Mg; 1000 mg/kg), a low magnesium content diet (L-Mg; 60 mg/kg), and a high magnesium content diet (H-Mg; 6000 mg/kg), for two weeks. After treatment, fecal samples were collected. Microbiota composition was assessed by sequencing the V3–V4 hypervariable region. Results: The C-Mg and L-Mg groups had more diversity than H-Mg group. CF231, SMB53, <i>Dorea</i>, <i>Lactobacillus</i> and <i>Turibacter</i> were enriched in the L-Mg group. In contrast, the phyla <i>Proteobacteria</i>, <i>Parabacteroides</i>, <i>Butyricimonas</i>, and <i>Victivallis</i> were overrepresented in the H-Mg group. PICRUSt analysis indicated that fecal microbiota of the L-Mg group were encoded with an increased abundance of metabolic pathways involving carbohydrate metabolism and butanoate metabolism. Conclusion: Dietary magnesium supplementation can result in intestinal dysbiosis development in a situation where there is no magnesium deficiency. Conversely, low dietary magnesium consumption is associated with microbiota with a higher capacity to harvest energy from the diet.https://www.mdpi.com/2072-6643/12/9/2889dietary magnesiummineralsgut microbiotaWistar male rat
spellingShingle Arantxa García-Legorreta
Luis Alfonso Soriano-Pérez
Aline Mariana Flores-Buendía
Omar Noel Medina-Campos
Lilia G. Noriega
Omar Granados-Portillo
Rafael Nambo-Venegas
Armando R. Tovar
Alfredo Mendoza-Vargas
Diana Barrera-Oviedo
José Pedraza-Chaverri
Berenice Palacios-González
Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats
Nutrients
dietary magnesium
minerals
gut microbiota
Wistar male rat
title Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats
title_full Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats
title_fullStr Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats
title_full_unstemmed Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats
title_short Effect of Dietary Magnesium Content on Intestinal Microbiota of Rats
title_sort effect of dietary magnesium content on intestinal microbiota of rats
topic dietary magnesium
minerals
gut microbiota
Wistar male rat
url https://www.mdpi.com/2072-6643/12/9/2889
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