Analgesic-like activity of perillyl acetate: In vivo and in silico studies
Objective: To evaluate the antinociceptive activity of perillyl acetate in mice and in silico simulations. Methods: The vehicle, perillyl acetate (100, 150 and/or 200 mg/ kg, i.p.), diazepam (2 mg/kg, i.p.) or morphine (6 mg/kg, i.p.) was administered to mice, respectively. Rotarod test, acetic acid...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2022-01-01
|
| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=4;spage=156;epage=163;aulast=Braga |
| _version_ | 1818274640772988928 |
|---|---|
| author | Renan Braga Humberto Andrade Ryldene Cruz Mayara Maia Carolina Lima Anderson Santos André Miranda Allana Duarte Marcus Scotti Reinaldo Almeida Damião Sousa |
| author_facet | Renan Braga Humberto Andrade Ryldene Cruz Mayara Maia Carolina Lima Anderson Santos André Miranda Allana Duarte Marcus Scotti Reinaldo Almeida Damião Sousa |
| author_sort | Renan Braga |
| collection | DOAJ |
| description | Objective: To evaluate the antinociceptive activity of perillyl acetate in mice and in silico simulations.
Methods: The vehicle, perillyl acetate (100, 150 and/or 200 mg/ kg, i.p.), diazepam (2 mg/kg, i.p.) or morphine (6 mg/kg, i.p.) was administered to mice, respectively. Rotarod test, acetic acid-induced abdominal writhing, formalin-induced nociception, hot plate test, and tail-flick test were performed. Opioid receptors-involvement in perillyl acetate antinociceptive effect was also investigated.
Results: Perillyl acetate did not affect the motor coordination of mice. However, it reduced the number of acetic acid-induced abdominal twitches and licking times in the formalin test. There was an increase of latency time in the tail-flick test of 30 and 60 minutes. Pretreatment with naloxone reversed the antinociceptive effect of perillyl acetate (200 mg/kg). In silico analysis demonstrated that perillyl acetate could bind to μ-opioid receptors.
Conclusions: Perillyl acetate has antinociceptive effect at the spinal level in animal nociception models, without affecting the locomotor integrity and possibly through μ-opioid receptors. In silico studies have suggested that perillyl acetate can act as a μ-opioid receptor agonist. |
| first_indexed | 2024-12-12T22:17:05Z |
| format | Article |
| id | doaj.art-677152d8d19f4a99bac1a852437eb596 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 2588-9222 |
| language | English |
| last_indexed | 2024-12-12T22:17:05Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-677152d8d19f4a99bac1a852437eb5962022-12-22T00:10:03ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222022-01-0112415616310.4103/2221-1691.340560Analgesic-like activity of perillyl acetate: In vivo and in silico studiesRenan BragaHumberto AndradeRyldene CruzMayara MaiaCarolina LimaAnderson SantosAndré MirandaAllana DuarteMarcus ScottiReinaldo AlmeidaDamião SousaObjective: To evaluate the antinociceptive activity of perillyl acetate in mice and in silico simulations. Methods: The vehicle, perillyl acetate (100, 150 and/or 200 mg/ kg, i.p.), diazepam (2 mg/kg, i.p.) or morphine (6 mg/kg, i.p.) was administered to mice, respectively. Rotarod test, acetic acid-induced abdominal writhing, formalin-induced nociception, hot plate test, and tail-flick test were performed. Opioid receptors-involvement in perillyl acetate antinociceptive effect was also investigated. Results: Perillyl acetate did not affect the motor coordination of mice. However, it reduced the number of acetic acid-induced abdominal twitches and licking times in the formalin test. There was an increase of latency time in the tail-flick test of 30 and 60 minutes. Pretreatment with naloxone reversed the antinociceptive effect of perillyl acetate (200 mg/kg). In silico analysis demonstrated that perillyl acetate could bind to μ-opioid receptors. Conclusions: Perillyl acetate has antinociceptive effect at the spinal level in animal nociception models, without affecting the locomotor integrity and possibly through μ-opioid receptors. In silico studies have suggested that perillyl acetate can act as a μ-opioid receptor agonist.http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=4;spage=156;epage=163;aulast=Bragaanalgesic; biological products; opioid; essential oil; pain; monoterpene; perillyl acetate |
| spellingShingle | Renan Braga Humberto Andrade Ryldene Cruz Mayara Maia Carolina Lima Anderson Santos André Miranda Allana Duarte Marcus Scotti Reinaldo Almeida Damião Sousa Analgesic-like activity of perillyl acetate: In vivo and in silico studies Asian Pacific Journal of Tropical Biomedicine analgesic; biological products; opioid; essential oil; pain; monoterpene; perillyl acetate |
| title | Analgesic-like activity of perillyl acetate: In vivo and in silico studies |
| title_full | Analgesic-like activity of perillyl acetate: In vivo and in silico studies |
| title_fullStr | Analgesic-like activity of perillyl acetate: In vivo and in silico studies |
| title_full_unstemmed | Analgesic-like activity of perillyl acetate: In vivo and in silico studies |
| title_short | Analgesic-like activity of perillyl acetate: In vivo and in silico studies |
| title_sort | analgesic like activity of perillyl acetate in vivo and in silico studies |
| topic | analgesic; biological products; opioid; essential oil; pain; monoterpene; perillyl acetate |
| url | http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=4;spage=156;epage=163;aulast=Braga |
| work_keys_str_mv | AT renanbraga analgesiclikeactivityofperillylacetateinvivoandinsilicostudies AT humbertoandrade analgesiclikeactivityofperillylacetateinvivoandinsilicostudies AT ryldenecruz analgesiclikeactivityofperillylacetateinvivoandinsilicostudies AT mayaramaia analgesiclikeactivityofperillylacetateinvivoandinsilicostudies AT carolinalima analgesiclikeactivityofperillylacetateinvivoandinsilicostudies AT andersonsantos analgesiclikeactivityofperillylacetateinvivoandinsilicostudies AT andremiranda analgesiclikeactivityofperillylacetateinvivoandinsilicostudies AT allanaduarte analgesiclikeactivityofperillylacetateinvivoandinsilicostudies AT marcusscotti analgesiclikeactivityofperillylacetateinvivoandinsilicostudies AT reinaldoalmeida analgesiclikeactivityofperillylacetateinvivoandinsilicostudies AT damiaosousa analgesiclikeactivityofperillylacetateinvivoandinsilicostudies |