Murine brain tumor microenvironment immunophenotyping using mass cytometry

Murine brain tumor microenvironment immunophenotyping using mass cytometry

Summary: Here, we present a mass cytometry protocol optimized to examine the phenotype of immune cells within the mouse glioma microenvironment, using a Sleeping Beauty transposon-mediated mouse glioma model. We describe antibody conjugation and titrations for analysis of immune cells. We then detai...

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Bibliographic Details
Main Authors: Brandon L. McClellan, Mahmoud S. Alghamri, Rohit Thalla, Pedro R. Lowenstein, Maria G. Castro
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Antibody
Cancer
Cell Biology
Flow Cytometry/Mass Cytometry
Immunology
Model Organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002374

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http://www.sciencedirect.com/science/article/pii/S2666166722002374

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