YAP/TAZ Promote Fibrotic Activity in Human Trabecular Meshwork Cells by Sensing Cytoskeleton Structure Alternation
Trabecular meshwork (TM) is the main channel of aqueous humor (AH) outflow and the crucial tissue responsible for intraocular pressure (IOP) regulation. The aberrant fibrotic activity of human TM (HTM) cells is thought to be partially responsible for the increased resistance to AH outflow and elevat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/10/7/235 |
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| author | Shan Huang Zhicheng Liu Xiuqing Qian Lin Li Haixia Zhang Shanshan Li Zhicheng Liu |
| author_facet | Shan Huang Zhicheng Liu Xiuqing Qian Lin Li Haixia Zhang Shanshan Li Zhicheng Liu |
| author_sort | Shan Huang |
| collection | DOAJ |
| description | Trabecular meshwork (TM) is the main channel of aqueous humor (AH) outflow and the crucial tissue responsible for intraocular pressure (IOP) regulation. The aberrant fibrotic activity of human TM (HTM) cells is thought to be partially responsible for the increased resistance to AH outflow and elevated IOP. This study aimed to identify the TM cell fibrotic activity biomarker and illustrate the mechanisms of fibrotic activity regulation in HTM cells. We used TGFβ2-treated HTM cells and detected the changes in the cytoskeletal structure, the Yes-associated protein (YAP) and its transcriptional co-activator with PDZ-binding domain (TAZ) activation, and the expression levels of the fibrosis-related proteins Collagen I and α-SMA in HTM cells by immunofluorescence staining or western bolt analyses. The expression of YAP was inhibited using siRNA transfection. The results showed that the expression levels of YAP/TAZ and the fibrosis-related proteins Collagen I and α-SMA in HTM cells were elevated under TGF-β2 treatment, which was correlated with the structural change of the cellular F-actin cytoskeleton. Furthermore, the inhibition of YAP decreased the expression of connective tissue growth factor (CTGF), Collagen I, and α-SMA in HTM cells. These findings demonstrate that YAP/TAZ are potential biomarkers in evaluating the TM cell fibrotic activity, and it could sense cytoskeletal structure cues and regulate the fibrotic activity of TM cells. |
| first_indexed | 2024-03-09T03:34:34Z |
| format | Article |
| id | doaj.art-67b48f086bda467e8bc875a2620ce4fd |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-09T03:34:34Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-67b48f086bda467e8bc875a2620ce4fd2023-12-03T14:50:12ZengMDPI AGChemosensors2227-90402022-06-0110723510.3390/chemosensors10070235YAP/TAZ Promote Fibrotic Activity in Human Trabecular Meshwork Cells by Sensing Cytoskeleton Structure AlternationShan Huang0Zhicheng Liu1Xiuqing Qian2Lin Li3Haixia Zhang4Shanshan Li5Zhicheng Liu6School of Biomedical Engineering, Capital Medical University, Beijing 100069, ChinaSchool of Biomedical Engineering, Capital Medical University, Beijing 100069, ChinaSchool of Biomedical Engineering, Capital Medical University, Beijing 100069, ChinaSchool of Biomedical Engineering, Capital Medical University, Beijing 100069, ChinaSchool of Biomedical Engineering, Capital Medical University, Beijing 100069, ChinaSchool of Biomedical Engineering, Capital Medical University, Beijing 100069, ChinaSchool of Biomedical Engineering, Capital Medical University, Beijing 100069, ChinaTrabecular meshwork (TM) is the main channel of aqueous humor (AH) outflow and the crucial tissue responsible for intraocular pressure (IOP) regulation. The aberrant fibrotic activity of human TM (HTM) cells is thought to be partially responsible for the increased resistance to AH outflow and elevated IOP. This study aimed to identify the TM cell fibrotic activity biomarker and illustrate the mechanisms of fibrotic activity regulation in HTM cells. We used TGFβ2-treated HTM cells and detected the changes in the cytoskeletal structure, the Yes-associated protein (YAP) and its transcriptional co-activator with PDZ-binding domain (TAZ) activation, and the expression levels of the fibrosis-related proteins Collagen I and α-SMA in HTM cells by immunofluorescence staining or western bolt analyses. The expression of YAP was inhibited using siRNA transfection. The results showed that the expression levels of YAP/TAZ and the fibrosis-related proteins Collagen I and α-SMA in HTM cells were elevated under TGF-β2 treatment, which was correlated with the structural change of the cellular F-actin cytoskeleton. Furthermore, the inhibition of YAP decreased the expression of connective tissue growth factor (CTGF), Collagen I, and α-SMA in HTM cells. These findings demonstrate that YAP/TAZ are potential biomarkers in evaluating the TM cell fibrotic activity, and it could sense cytoskeletal structure cues and regulate the fibrotic activity of TM cells.https://www.mdpi.com/2227-9040/10/7/235trabecular meshworkextracellular matrixfibrotic activitycytoskeleton |
| spellingShingle | Shan Huang Zhicheng Liu Xiuqing Qian Lin Li Haixia Zhang Shanshan Li Zhicheng Liu YAP/TAZ Promote Fibrotic Activity in Human Trabecular Meshwork Cells by Sensing Cytoskeleton Structure Alternation Chemosensors trabecular meshwork extracellular matrix fibrotic activity cytoskeleton |
| title | YAP/TAZ Promote Fibrotic Activity in Human Trabecular Meshwork Cells by Sensing Cytoskeleton Structure Alternation |
| title_full | YAP/TAZ Promote Fibrotic Activity in Human Trabecular Meshwork Cells by Sensing Cytoskeleton Structure Alternation |
| title_fullStr | YAP/TAZ Promote Fibrotic Activity in Human Trabecular Meshwork Cells by Sensing Cytoskeleton Structure Alternation |
| title_full_unstemmed | YAP/TAZ Promote Fibrotic Activity in Human Trabecular Meshwork Cells by Sensing Cytoskeleton Structure Alternation |
| title_short | YAP/TAZ Promote Fibrotic Activity in Human Trabecular Meshwork Cells by Sensing Cytoskeleton Structure Alternation |
| title_sort | yap taz promote fibrotic activity in human trabecular meshwork cells by sensing cytoskeleton structure alternation |
| topic | trabecular meshwork extracellular matrix fibrotic activity cytoskeleton |
| url | https://www.mdpi.com/2227-9040/10/7/235 |
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