A Robust Expression and Purification Method for Production of <i>Sp</i>Cas9-GFP-MBP Fusion Protein for In Vitro Applications
Genome editing using the CRISPR/Cas9 system is one of the trendiest methodologies in the scientific community. Many genome editing approaches require recombinant <i>Streptococcus pyogenes</i> Cas9 (<i>Sp</i>Cas9) at some point during their application, for instance, for in vi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
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| Series: | Methods and Protocols |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2409-9279/5/3/44 |