A Robust Expression and Purification Method for Production of <i>Sp</i>Cas9-GFP-MBP Fusion Protein for In Vitro Applications

A Robust Expression and Purification Method for Production of <i>Sp</i>Cas9-GFP-MBP Fusion Protein for In Vitro Applications

Genome editing using the CRISPR/Cas9 system is one of the trendiest methodologies in the scientific community. Many genome editing approaches require recombinant <i>Streptococcus pyogenes</i> Cas9 (<i>Sp</i>Cas9) at some point during their application, for instance, for in vi...

Full description

Bibliographic Details
Main Authors: Andrea Luciana Fleitas, Mario Señorale, Sabina Vidal
Format: Article
Language:English
Published: MDPI AG 2022-05-01
Series:Methods and Protocols
Subjects:
CIRSPR/Cas9
expression
purification
immobilized metal affinity chromatography
ion exchange
Online Access:https://www.mdpi.com/2409-9279/5/3/44

Similar Items

Similar Items