Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone Receptor
Androgenetic alopecia (AGA) is the most common type of hair loss, and is mainly caused by the biological effects of testosterone on dermal papilla cells (DPCs). In vitro culturing of DPCs might be a useful tool for the screening of target molecule of AGA. However, primary DPCs cannot continuously pr...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-03-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fcell.2020.00157/full |
| _version_ | 1818334401105231872 |
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| author | Tomokazu Fukuda Tomokazu Fukuda Kouhei Takahashi Shin Takase Ai Orimoto Takahiro Eitsuka Kiyotaka Nakagawa Tohru Kiyono |
| author_facet | Tomokazu Fukuda Tomokazu Fukuda Kouhei Takahashi Shin Takase Ai Orimoto Takahiro Eitsuka Kiyotaka Nakagawa Tohru Kiyono |
| author_sort | Tomokazu Fukuda |
| collection | DOAJ |
| description | Androgenetic alopecia (AGA) is the most common type of hair loss, and is mainly caused by the biological effects of testosterone on dermal papilla cells (DPCs). In vitro culturing of DPCs might be a useful tool for the screening of target molecule of AGA. However, primary DPCs cannot continuously proliferate owing to cellular senescence and cell culture stress. In this study, we introduced mutant cyclin-dependent kinase 4 (CDK4), Cyclin D1, and telomerase reverse transcriptase (TERT) into DPCs. We confirmed protein expression of CDK4 and Cyclin D1, and enzymatic activity of TERT. Furthermore, we found the established cell line was free from cellular senescence. We also introduced the androgen receptor gene using a recombinant retrovirus, to compensate the transcriptional suppressed endogenous androgen receptor in the process of cell proliferation. Furthermore, we detected the efficient nuclear translocation of androgen receptor into the nucleus after the treatment of dihydrotestosterone, indicating the functionality of our introduced receptor. Our established cell line is a useful tool to identify the downstream signaling pathway, which activated by the testosterone. |
| first_indexed | 2024-12-13T14:06:56Z |
| format | Article |
| id | doaj.art-67d1c6321d5b461b8b98f3bc93fbfbf8 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-13T14:06:56Z |
| publishDate | 2020-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-67d1c6321d5b461b8b98f3bc93fbfbf82022-12-21T23:42:34ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-03-01810.3389/fcell.2020.00157519794Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone ReceptorTomokazu Fukuda0Tomokazu Fukuda1Kouhei Takahashi2Shin Takase3Ai Orimoto4Takahiro Eitsuka5Kiyotaka Nakagawa6Tohru Kiyono7Graduate School of Science and Engineering, Iwate University, Morioka, JapanSoft-Path Engineering Research Center, Iwate University, Morioka, JapanGraduate School of Science and Engineering, Iwate University, Morioka, JapanGraduate School of Science and Engineering, Iwate University, Morioka, JapanGraduate School of Science and Engineering, Iwate University, Morioka, JapanGraduate School of Agricultural Science, Tohoku University, Sendai, JapanGraduate School of Agricultural Science, Tohoku University, Sendai, JapanDivision of Carcinogenesis and Cancer Prevention and Department of Cell Culture Technology, National Cancer Center Research Institute, Tokyo, JapanAndrogenetic alopecia (AGA) is the most common type of hair loss, and is mainly caused by the biological effects of testosterone on dermal papilla cells (DPCs). In vitro culturing of DPCs might be a useful tool for the screening of target molecule of AGA. However, primary DPCs cannot continuously proliferate owing to cellular senescence and cell culture stress. In this study, we introduced mutant cyclin-dependent kinase 4 (CDK4), Cyclin D1, and telomerase reverse transcriptase (TERT) into DPCs. We confirmed protein expression of CDK4 and Cyclin D1, and enzymatic activity of TERT. Furthermore, we found the established cell line was free from cellular senescence. We also introduced the androgen receptor gene using a recombinant retrovirus, to compensate the transcriptional suppressed endogenous androgen receptor in the process of cell proliferation. Furthermore, we detected the efficient nuclear translocation of androgen receptor into the nucleus after the treatment of dihydrotestosterone, indicating the functionality of our introduced receptor. Our established cell line is a useful tool to identify the downstream signaling pathway, which activated by the testosterone.https://www.frontiersin.org/article/10.3389/fcell.2020.00157/fulldihydrotestosteronedermal papilla cellsimmortalizationandrogen receptornuclear localization |
| spellingShingle | Tomokazu Fukuda Tomokazu Fukuda Kouhei Takahashi Shin Takase Ai Orimoto Takahiro Eitsuka Kiyotaka Nakagawa Tohru Kiyono Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone Receptor Frontiers in Cell and Developmental Biology dihydrotestosterone dermal papilla cells immortalization androgen receptor nuclear localization |
| title | Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone Receptor |
| title_full | Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone Receptor |
| title_fullStr | Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone Receptor |
| title_full_unstemmed | Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone Receptor |
| title_short | Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone Receptor |
| title_sort | human derived immortalized dermal papilla cells with a constant expression of testosterone receptor |
| topic | dihydrotestosterone dermal papilla cells immortalization androgen receptor nuclear localization |
| url | https://www.frontiersin.org/article/10.3389/fcell.2020.00157/full |
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