Toxic effects of trace phenol/guanidine isothiocyanate (P/GI) on cells cultured nearby in covered 96-well plates

Abstract Background A mixture of phenol and guanidine isothiocyanate (“P/GI”, the principal components of TRIzol™ and similar products) is routinely used to isolate RNA, DNA, and proteins from a single specimen. In time-course experiments of cells grown in tissue culture, replicate wells are often h...

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Bibliographic Details
Main Authors: Madeline Snedden, Lavisha Singh, Chandrashekara Kyathanahalli, Emmet Hirsch
Format: Article
Language:English
Published: BMC 2022-11-01
Series:BMC Biotechnology
Subjects:
Online Access:https://doi.org/10.1186/s12896-022-00766-2