Mechanistic target of rapamycin (Mtor) is essential for murine embryonic heart development and growth.
Mechanistic target of rapamycin (Mtor) is required for embryonic inner cell mass proliferation during early development. However, Mtor expression levels are very low in the mouse heart during embryogenesis. To determine if Mtor plays a role during mouse cardiac development, cardiomyocyte specific Mt...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3544830?pdf=render |
| _version_ | 1819036047945760768 |
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| author | Yi Zhu Karla M P Pires Kevin J Whitehead Curtis D Olsen Benjamin Wayment Yi Cheng Zhang Heiko Bugger Olesya Ilkun Sheldon E Litwin George Thomas Sara C Kozma E Dale Abel |
| author_facet | Yi Zhu Karla M P Pires Kevin J Whitehead Curtis D Olsen Benjamin Wayment Yi Cheng Zhang Heiko Bugger Olesya Ilkun Sheldon E Litwin George Thomas Sara C Kozma E Dale Abel |
| author_sort | Yi Zhu |
| collection | DOAJ |
| description | Mechanistic target of rapamycin (Mtor) is required for embryonic inner cell mass proliferation during early development. However, Mtor expression levels are very low in the mouse heart during embryogenesis. To determine if Mtor plays a role during mouse cardiac development, cardiomyocyte specific Mtor deletion was achieved using α myosin heavy chain (α-MHC) driven Cre recombinase. Initial mosaic expression of Cre between embryonic day (E) 10.5 and E11.5 eliminated a subset of cardiomyocytes with high Cre activity by apoptosis and reduced overall cardiac proliferative capacity. The remaining cardiomyocytes proliferated and expanded normally. However loss of 50% of cardiomyocytes defined a threshold that impairs the ability of the embryonic heart to sustain the embryo's circulatory requirements. As a result 92% of embryos with cardiomyocyte Mtor deficiency died by the end of gestation. Thus Mtor is required for survival and proliferation of cardiomyocytes in the developing heart. |
| first_indexed | 2024-12-21T07:59:19Z |
| format | Article |
| id | doaj.art-681023591c0449bf9af79bd49c3263a2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T07:59:19Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-681023591c0449bf9af79bd49c3263a22022-12-21T19:10:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e5422110.1371/journal.pone.0054221Mechanistic target of rapamycin (Mtor) is essential for murine embryonic heart development and growth.Yi ZhuKarla M P PiresKevin J WhiteheadCurtis D OlsenBenjamin WaymentYi Cheng ZhangHeiko BuggerOlesya IlkunSheldon E LitwinGeorge ThomasSara C KozmaE Dale AbelMechanistic target of rapamycin (Mtor) is required for embryonic inner cell mass proliferation during early development. However, Mtor expression levels are very low in the mouse heart during embryogenesis. To determine if Mtor plays a role during mouse cardiac development, cardiomyocyte specific Mtor deletion was achieved using α myosin heavy chain (α-MHC) driven Cre recombinase. Initial mosaic expression of Cre between embryonic day (E) 10.5 and E11.5 eliminated a subset of cardiomyocytes with high Cre activity by apoptosis and reduced overall cardiac proliferative capacity. The remaining cardiomyocytes proliferated and expanded normally. However loss of 50% of cardiomyocytes defined a threshold that impairs the ability of the embryonic heart to sustain the embryo's circulatory requirements. As a result 92% of embryos with cardiomyocyte Mtor deficiency died by the end of gestation. Thus Mtor is required for survival and proliferation of cardiomyocytes in the developing heart.http://europepmc.org/articles/PMC3544830?pdf=render |
| spellingShingle | Yi Zhu Karla M P Pires Kevin J Whitehead Curtis D Olsen Benjamin Wayment Yi Cheng Zhang Heiko Bugger Olesya Ilkun Sheldon E Litwin George Thomas Sara C Kozma E Dale Abel Mechanistic target of rapamycin (Mtor) is essential for murine embryonic heart development and growth. PLoS ONE |
| title | Mechanistic target of rapamycin (Mtor) is essential for murine embryonic heart development and growth. |
| title_full | Mechanistic target of rapamycin (Mtor) is essential for murine embryonic heart development and growth. |
| title_fullStr | Mechanistic target of rapamycin (Mtor) is essential for murine embryonic heart development and growth. |
| title_full_unstemmed | Mechanistic target of rapamycin (Mtor) is essential for murine embryonic heart development and growth. |
| title_short | Mechanistic target of rapamycin (Mtor) is essential for murine embryonic heart development and growth. |
| title_sort | mechanistic target of rapamycin mtor is essential for murine embryonic heart development and growth |
| url | http://europepmc.org/articles/PMC3544830?pdf=render |
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