Injured adult retinal axons with Pten and Socs3 co-deletion reform active synapses with suprachiasmatic neurons
Despite advances in promoting axonal regeneration after adult central nervous system injury, elicitation of a large number of lesion-passing axons reform active synaptic connections with natural target neurons remains limited. By deleting both Pten and Socs3 in retinal ganglion cells, we report that...
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015-01-01
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| Series: | Neurobiology of Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996114002927 |
| _version_ | 1818869963222417408 |
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| author | Songshan Li Qinghai He Hao Wang Xuming Tang Kam Wing Ho Xin Gao Qian Zhang Yang Shen Annie Cheung Francis Wong Yung Hou Wong Nancy Y. Ip Liwen Jiang Wing Ho Yung Kai Liu |
| author_facet | Songshan Li Qinghai He Hao Wang Xuming Tang Kam Wing Ho Xin Gao Qian Zhang Yang Shen Annie Cheung Francis Wong Yung Hou Wong Nancy Y. Ip Liwen Jiang Wing Ho Yung Kai Liu |
| author_sort | Songshan Li |
| collection | DOAJ |
| description | Despite advances in promoting axonal regeneration after adult central nervous system injury, elicitation of a large number of lesion-passing axons reform active synaptic connections with natural target neurons remains limited. By deleting both Pten and Socs3 in retinal ganglion cells, we report that optic nerve axons after prechiasm lesion robustly reinnervate the hypothalamus, form new synapses with neurons in the suprachiasmatic nucleus (SCN), and re-integrate with the existing circuitry. Photic or electric stimulation of the retinal axons induces neuronal response in SCN. However both the innervation pattern and evoked responses are not completely restored by the regenerating axons, suggesting that combining with other strategies is necessary to overcome the defective rewiring. Our results support that boosting the intrinsic growth capacity in injured neurons promotes axonal reinnervation and rewiring. |
| first_indexed | 2024-12-19T11:59:28Z |
| format | Article |
| id | doaj.art-68286708873b409894b25f4e6d9722d8 |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-12-19T11:59:28Z |
| publishDate | 2015-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-68286708873b409894b25f4e6d9722d82022-12-21T20:22:32ZengElsevierNeurobiology of Disease1095-953X2015-01-0173366376Injured adult retinal axons with Pten and Socs3 co-deletion reform active synapses with suprachiasmatic neuronsSongshan Li0Qinghai He1Hao Wang2Xuming Tang3Kam Wing Ho4Xin Gao5Qian Zhang6Yang Shen7Annie Cheung8Francis Wong9Yung Hou Wong10Nancy Y. Ip11Liwen Jiang12Wing Ho Yung13Kai Liu14Division of Life Science, State Key Laboratory of Molecular Neuroscience, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China; Center of Systems Biology and Human Health, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, ChinaDivision of Life Science, State Key Laboratory of Molecular Neuroscience, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China; Center of Systems Biology and Human Health, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, ChinaSchool of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, ChinaDivision of Life Science, State Key Laboratory of Molecular Neuroscience, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China; Center of Systems Biology and Human Health, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, ChinaSchool of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, ChinaDivision of Life Science, State Key Laboratory of Molecular Neuroscience, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China; Center of Systems Biology and Human Health, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, ChinaDivision of Life Science, State Key Laboratory of Molecular Neuroscience, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China; Center of Systems Biology and Human Health, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, ChinaDivision of Life Science, State Key Laboratory of Molecular Neuroscience, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China; Center of Systems Biology and Human Health, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, ChinaDivision of Life Science, State Key Laboratory of Molecular Neuroscience, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, ChinaDivision of Life Science, State Key Laboratory of Molecular Neuroscience, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China; Center of Systems Biology and Human Health, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, ChinaDivision of Life Science, State Key Laboratory of Molecular Neuroscience, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, ChinaDivision of Life Science, State Key Laboratory of Molecular Neuroscience, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China; Center of Systems Biology and Human Health, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, ChinaSchool of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, ChinaSchool of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, ChinaDivision of Life Science, State Key Laboratory of Molecular Neuroscience, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China; Center of Systems Biology and Human Health, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China; Corresponding author at: Division of Life Science, State Key Laboratory of Molecular Neuroscience, School of Science and Institute for Advance Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.Despite advances in promoting axonal regeneration after adult central nervous system injury, elicitation of a large number of lesion-passing axons reform active synaptic connections with natural target neurons remains limited. By deleting both Pten and Socs3 in retinal ganglion cells, we report that optic nerve axons after prechiasm lesion robustly reinnervate the hypothalamus, form new synapses with neurons in the suprachiasmatic nucleus (SCN), and re-integrate with the existing circuitry. Photic or electric stimulation of the retinal axons induces neuronal response in SCN. However both the innervation pattern and evoked responses are not completely restored by the regenerating axons, suggesting that combining with other strategies is necessary to overcome the defective rewiring. Our results support that boosting the intrinsic growth capacity in injured neurons promotes axonal reinnervation and rewiring.http://www.sciencedirect.com/science/article/pii/S0969996114002927Axon regeneration and rewiringIntrinsic mechanismsPre-chiasm lesionRetinal ganglion cellsSuprachiasmatic nucleus |
| spellingShingle | Songshan Li Qinghai He Hao Wang Xuming Tang Kam Wing Ho Xin Gao Qian Zhang Yang Shen Annie Cheung Francis Wong Yung Hou Wong Nancy Y. Ip Liwen Jiang Wing Ho Yung Kai Liu Injured adult retinal axons with Pten and Socs3 co-deletion reform active synapses with suprachiasmatic neurons Neurobiology of Disease Axon regeneration and rewiring Intrinsic mechanisms Pre-chiasm lesion Retinal ganglion cells Suprachiasmatic nucleus |
| title | Injured adult retinal axons with Pten and Socs3 co-deletion reform active synapses with suprachiasmatic neurons |
| title_full | Injured adult retinal axons with Pten and Socs3 co-deletion reform active synapses with suprachiasmatic neurons |
| title_fullStr | Injured adult retinal axons with Pten and Socs3 co-deletion reform active synapses with suprachiasmatic neurons |
| title_full_unstemmed | Injured adult retinal axons with Pten and Socs3 co-deletion reform active synapses with suprachiasmatic neurons |
| title_short | Injured adult retinal axons with Pten and Socs3 co-deletion reform active synapses with suprachiasmatic neurons |
| title_sort | injured adult retinal axons with pten and socs3 co deletion reform active synapses with suprachiasmatic neurons |
| topic | Axon regeneration and rewiring Intrinsic mechanisms Pre-chiasm lesion Retinal ganglion cells Suprachiasmatic nucleus |
| url | http://www.sciencedirect.com/science/article/pii/S0969996114002927 |
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