Central carbon metabolite profiling reveals vector-associated differences in the recombinant protein production host Escherichia coli BL21

Central carbon metabolite profiling reveals vector-associated differences in the recombinant protein production host Escherichia coli BL21

The Gram-negative bacterium Escherichia coli is the most widely used host for recombinant protein production, both as an industrial expression platform and as a model system at laboratory scale. The recombinant protein production industry generates proteins with direct applications as biopharmaceuti...

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Bibliographic Details
Main Authors: Laura García-Calvo, Divyata Vilas Rane, Nikalet Everson, Sigurd Tømmerberg Humlebrekk, Lise Femanger Mathiassen, Astfrid Helene Morka Mæhlum, Jostein Malmo, Per Bruheim
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-03-01
Series:Frontiers in Chemical Engineering
Subjects:
Escherichia coli
recombinant protein production
metabolic burden
central carbon metabolism
XylS/Pm
mass spectrometry
Online Access:https://www.frontiersin.org/articles/10.3389/fceng.2023.1142226/full

Internet

https://www.frontiersin.org/articles/10.3389/fceng.2023.1142226/full

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