Optimization of ultrasound-assisted extraction based on response surface methodology using HPLC-DAD for the analysis of red clover (Trifolium pretense L.) isoflavones and its anti-inflammatory activities on LPS-induced 3D4/2 cell

Optimization of ultrasound-assisted extraction based on response surface methodology using HPLC-DAD for the analysis of red clover (Trifolium pretense L.) isoflavones and its anti-inflammatory activities on LPS-induced 3D4/2 cell

IntroductionTrifolium pratense L. has anti-inflammatory, antioxidant, cardiovascular disease prevention, and estrogen-like effects. The existing method for the assay of effective components is commonly based on a spectrophotometer, which could not meet the requirement of quality control. Furthermore...

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Bibliographic Details
Main Authors: Zhengqin Luo, Yidan Xu, Longxin Qiu, Shiming Lv, Cheng Zeng, Aijuan Tan, Deyuan Ou, Xuqin Song, Jian Yang
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-10-01
Series:Frontiers in Veterinary Science
Subjects:
red clover
isoflavones
response surface methodology
high-performance liquid chromatography coupled with diode array detection
porcine alveolar macrophage (3D4/2)
anti-inflammatory
Online Access:https://www.frontiersin.org/articles/10.3389/fvets.2023.1279178/full

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https://www.frontiersin.org/articles/10.3389/fvets.2023.1279178/full

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