Preparation of Calcium-Binding Phosvitin Phosphopeptide and Structural Characterization of Its Calcium Chelate
To prepare a calcium-binding peptide as calcium supplement, phosvitin phosphopeptide (PPP) was prepared from egg yolk and chelated with calcium ions. Phosvitin (PV) was treated at high temperature and high pressure, and then sequentially hydrolyzed with trypsin and alkaline protease. Based on hydrol...
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| Format: | Article |
| Language: | English |
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China Food Publishing Company
2023-03-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-6-016.pdf |
| _version_ | 1827958229828632576 |
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| author | SONG Li, ZHU Linxian, SONG Lushan, SI Kai, GONG Tingting, LIU Huiping, ZHANG Xiaowei |
| author_facet | SONG Li, ZHU Linxian, SONG Lushan, SI Kai, GONG Tingting, LIU Huiping, ZHANG Xiaowei |
| author_sort | SONG Li, ZHU Linxian, SONG Lushan, SI Kai, GONG Tingting, LIU Huiping, ZHANG Xiaowei |
| collection | DOAJ |
| description | To prepare a calcium-binding peptide as calcium supplement, phosvitin phosphopeptide (PPP) was prepared from egg yolk and chelated with calcium ions. Phosvitin (PV) was treated at high temperature and high pressure, and then sequentially hydrolyzed with trypsin and alkaline protease. Based on hydrolysis degree and calcium binding efficiency of peptide, the hydrolysis conditions of alkaline protease were optimized. Phosvitin phosphopeptide-calcium chelate (PPP-Ca) was characterized by ultraviolet (UV) spectroscopy, infrared (IR) spectroscopy, circular dichroism (CD) spectroscopy, scanning electron microscopy (SEM) and zeta potential analysis. The results revealed that the optimal hydrolysis conditions were as follows: enzyme dosage 5%, hydrolysis time 90 min, pH 9.0 and 40 ℃. Under these conditions, the degree of hydrolysis was (25.45 ± 0.17)% and the calcium binding efficiency of peptide was (93.41 ± 1.10)%. The structural characterization showed that Ca bound to PPP mainly through interactions between carboxyl, amino and phosphate groups, and the structure of PPP-Ca was tightly ordered and had aggregation phenomenon. |
| first_indexed | 2024-04-09T15:33:18Z |
| format | Article |
| id | doaj.art-6863e157cd144a5aa845ec2b55f25b86 |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-04-09T15:33:18Z |
| publishDate | 2023-03-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-6863e157cd144a5aa845ec2b55f25b862023-04-28T05:11:21ZengChina Food Publishing CompanyShipin Kexue1002-66302023-03-0144612513310.7506/spkx1002-6630-20220414-167Preparation of Calcium-Binding Phosvitin Phosphopeptide and Structural Characterization of Its Calcium ChelateSONG Li, ZHU Linxian, SONG Lushan, SI Kai, GONG Tingting, LIU Huiping, ZHANG Xiaowei0(State Key Laboratory of Food Nutrition and Safety, Tianjin Key Laboratory of Food Quality and Health, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China)To prepare a calcium-binding peptide as calcium supplement, phosvitin phosphopeptide (PPP) was prepared from egg yolk and chelated with calcium ions. Phosvitin (PV) was treated at high temperature and high pressure, and then sequentially hydrolyzed with trypsin and alkaline protease. Based on hydrolysis degree and calcium binding efficiency of peptide, the hydrolysis conditions of alkaline protease were optimized. Phosvitin phosphopeptide-calcium chelate (PPP-Ca) was characterized by ultraviolet (UV) spectroscopy, infrared (IR) spectroscopy, circular dichroism (CD) spectroscopy, scanning electron microscopy (SEM) and zeta potential analysis. The results revealed that the optimal hydrolysis conditions were as follows: enzyme dosage 5%, hydrolysis time 90 min, pH 9.0 and 40 ℃. Under these conditions, the degree of hydrolysis was (25.45 ± 0.17)% and the calcium binding efficiency of peptide was (93.41 ± 1.10)%. The structural characterization showed that Ca bound to PPP mainly through interactions between carboxyl, amino and phosphate groups, and the structure of PPP-Ca was tightly ordered and had aggregation phenomenon.https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-6-016.pdfphosvitin phosphopeptide; peptide-calcium chelate; sequential enzymatic hydrolysis; structural characterization |
| spellingShingle | SONG Li, ZHU Linxian, SONG Lushan, SI Kai, GONG Tingting, LIU Huiping, ZHANG Xiaowei Preparation of Calcium-Binding Phosvitin Phosphopeptide and Structural Characterization of Its Calcium Chelate Shipin Kexue phosvitin phosphopeptide; peptide-calcium chelate; sequential enzymatic hydrolysis; structural characterization |
| title | Preparation of Calcium-Binding Phosvitin Phosphopeptide and Structural Characterization of Its Calcium Chelate |
| title_full | Preparation of Calcium-Binding Phosvitin Phosphopeptide and Structural Characterization of Its Calcium Chelate |
| title_fullStr | Preparation of Calcium-Binding Phosvitin Phosphopeptide and Structural Characterization of Its Calcium Chelate |
| title_full_unstemmed | Preparation of Calcium-Binding Phosvitin Phosphopeptide and Structural Characterization of Its Calcium Chelate |
| title_short | Preparation of Calcium-Binding Phosvitin Phosphopeptide and Structural Characterization of Its Calcium Chelate |
| title_sort | preparation of calcium binding phosvitin phosphopeptide and structural characterization of its calcium chelate |
| topic | phosvitin phosphopeptide; peptide-calcium chelate; sequential enzymatic hydrolysis; structural characterization |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-6-016.pdf |
| work_keys_str_mv | AT songlizhulinxiansonglushansikaigongtingtingliuhuipingzhangxiaowei preparationofcalciumbindingphosvitinphosphopeptideandstructuralcharacterizationofitscalciumchelate |